J Chromatogr A, 1684, 463582 (2022). Samples were concentrated filtrates of leachates of waste deposition sites, as well as testosterone, flutamide, bisphenol A (BPA) and nitroquinoline oxide (NQO) as standards. Automated Multiple Development on HPTLC silica gel (prewashed with methanol and dried 30 min at 110 °C) with 1) methanol up to 20 mm; 2A) chloroform – ethyl acetate –petroleum ether 11:4:5 or 2B) ethyl acetate – n-hexane 1:1 for flutamide and testosterone, up to 90 mm. Effect-directed analysis was performed by automated spraying 3 mL suspension of BJ1991 yeast (transfected Saccharomyces cerevisiae strain, pure for androgenic activity, with 50 ng/mL testosterone for anti-androgenic assay), followed by 20 h incubation at 30 °C in a closed chamber (90 % relative humidity), by 5 min drying under cold air stream, by spraying 2.5 mL MUG solution (4-methylumbelliferyl-galactopyranoside) and by 15 min incubation at 37 °C in an open chamber. Agonistic and antagonistic activities were detected qualitatively under UV 366 nm (light or dark blue bands, respectively, on blue background) and quantitatively documented using automated scanning at excitation wavelength 320 nm (deuterium lamp), with cut-off filter at 400 nm. Dose-response curves for model compounds were established by regression analysis. Anti-androgenic effective doses at 10 % were 28 ng/zone for flutamide and 20 ng/zone for BPA, without toxicity for the yeast. To exclude cytotoxicity where anti-androgenic activity was observed, the HPTLC layers (either without or after the spraying with MUG) were sprayed with 3 mL resazurin solution (0.01 % in water) and incubated 30 min at 30 °C and 90 % humidity. Cytotoxicity bands appeared as pink zones of resorufin on a colorless background (dihydroresorufin) under white light. Densitometric evaluation in absorption mode at 575 nm (under deuterium and halogen-tungsten lamps, no filter applied). NQO was cytotoxic at its lowest tested dose (1 ng/zone).

J. Liqu. Chromatogr. 23, 579-586 (2000). HPTLC of benzo[a]pyrene on hydrocarbon impregnated RP-18 plates with methanol - acetonitrile 1:1. Quantification by densitometry at 370 nm.

J. Agric. Food Chem. 49, 2363-2369 (2001). TLC of metalaxyl and [phenyl-14C] metalaxyl on soil plates: Plates prepared by grinding the soil samples, sieving and spreading the slurry over glass plates. Application of the samples on the plates stored overnight at a humidity of 70%. Distilled water was used as mobile phase. Detection by Bio-Image Analyzer.

J. Agric. Food Chem. 50, 4619-4627 (2002). TLC on silica gel of alachlor with n-hexane - ethyl acetate 1:1, of chlorpyrifos with n-hexane - toluene 7:3, of deltamethrin with toluene - cyclohexane 7:3, of endosulfan and endosulfan-sulfate with n-hexane - acetone 9:1, of monocrotophos with acetonitrile - water 3:2, of simazine with n-hexane - acetone 1:1, of triflurolin with n-hexane - acetone 3:1. The spatial distribution of radioactivity on the TLC-plates was measured and integrated by an Instant Image Analyzer.

J. Agric. Food Chem. 49, 2352-2358 (2001). TLC of metalaxyl (N-(2,6-dimethylphenyl)-N-(methoxyacetyl)-DL-alaninate) and metabolites on silica gel with ethyl acetate - 2-propanol - water 65:23:12. Measurement of the radioactive zones with a Bio-Image Analyzer.

J. Agric. Food Chem. 50, 5626-5633 (2002). TLC of isoxaflutole, [14C]-isoxaflutole and diketonitril-isoxaflutole on silica gel with ethyl acetate - methanol - acetic acid 92:5:4:3.

CBS 83, 6-7 (1999). HPTLC-AMD of PAHs in liver and lung of animals exposed to contaminated soil on RP-18 with a 14-step gradient from acetonitrile to methanol - water 9:1. Quantification by fluorescence measurement at 313/>400 nm and 254/>400 nm, respectively. Strict linearity (r2 > 0.99 on 5 measuring points) was proven. Coefficient of variation was found to be < 5 %, and recoveries between 55 and 70 % were given. Limits of detection was determined to be 0.2 to 2 ng/g sample.

J. Chromatogr. A 1526, 151-156 (2017). Development of a rapid and cost-effective method for determining brominated flame retardants (BFR) such as tetrabromobisphenol A/S (TBBPA/S) and their derivatives in soils, including TBBPA, TBBPA bis(allyl ether) (TBBPA-BAE), TBBPA bis(2,3-dibromopropyl ether) (TBBPA-BDBPE), TBBPS bis(allyl ether) (TBBPS-BAE) and TBBPS bis(2,3-dibromopropyl ether) (TBBPS-BDBPE), based on TLC sample pre-treatment coupled with HPLC-DAD at UV 214 nm. The LODs and LOQs were between 0.023-0.087 μg/g dw and 0.076-0.29 μg/g dw, respectively. The recoveries were between 41-108 % and both % RSD of repeatability and intermediate precision were < 11 %. The method showed a good performance for analyzing natural soil samples collected from BFRs industrial park, suggesting its great application potential for monitoring environmental TBBPA/S and their derivatives.