J. Agric. Food Chem. 64, 1087-1093 (2016). HPTLC of glycyrrhizin (1), liquiritin (2) and liquiritigenin (3) in Licorice on silica gel with 1-butanol – water – acetic acid 7:2:1. Detection by spraying with 10 % (v/v) sulfuric acid solution followed by heating at 105 °C for 5 min. Eastern blotting by spraying the plate with freshly prepared blotting solution comprising 2-propanol – methanol – water 1:5:10 and 0.05 % ammonia. The TLC plate was then covered with a polyethersulphone (PES) membrane and glass microfiber filter and all components were transferred to the PES membrane by press blotting for 65 s at 120 °C on a hot plate. The blotted PES membrane was dipped in sodium periodate solution (10 mg/mL) and left for 30 min. After rinsing with water, 1 % BSA in a 50 mM carbonate buffer (pH 9.6) was added and shaken for 3 h. Subsequently, the PES membrane was blocked with phosphate buffered saline (PBS) containing 5% skimmed milk to reduce the nonspecific binding reaction for 1 h. After washing three times with PBS containing 0.05 % Tween 20 (PBST), the membrane was immersed in the anti-Liq mAb solution for 3 h. Following the antigen–antibody complex reaction, the PES membrane was washed twice with PBST. Subsequently, a HRP-labeled antimouse IgG goat serum (whole molecule) solution diluted with PBST (1:500) was added and shaken for 1 h. After washing twice with PBST and once with PBS for 5 min, the PES membrane was exposed to a freshly prepared 4-chloro-1-naphthol/0.03 % hydrogen peroxide solution in PBS (1 mg/mL) for 10 min. The reaction was stopped by washing with PBS.

J. Chromatogr. 698, 281-299 (1995). A review with 68 references on the use of various types of gel electrophoresis for distinguishing between and identifying plant varieties, varying in genetic structure, with some practical applications. Discussion of the future trends for the use of electrophoresis in this area. Techniques: native polyacrylamide gel electrophoresis, sodium dodecyl sulfate PAGE, isoelectric focusing and two-dimensional methods are involved.

J. Chromatogr. A 744, 295-301 (1996). SDS-PAGE with a 4% acrylamide stacking gel and a 4-20% acrylamide gradient resolving gel. Visualization of the proteinbands with an Oakley silver stain. Comparison of the results with those obtained by capillary electrophoresis. Discussion of the feasibility of replacing the former with the latter in a quality control environment.

J. Planar Chromatogr. 16, 308-310 (2003). TLC of bovine serum albumin on silica gel impregnated by overnight predevelopment with chloroform - paraffin oil 19:1 with buffer solutions of pH 1, 4, 7, 10, or 12. After drying of the chromatogram visualization by treatment with a 2% solution of ninhydrin in acetone.

J. Chromatogr. Sci. 55 (5), 564-570 (2017). Determination of the chromatographic retention data for 13 new 5-arylidene-2,4-thiazolidinediones on cyano phase and RP-18 with 6 aqueous binary mobile phases modified with acetonitrile, methanol, ethanol, propanol, acetone and dioxane. Presentation of 3 attempts to find suitable quantitative structure–retention relationship (QSRR) models that quantify retention as a function of molecular descriptors. It was found that models built for RP-18 show generally better multiple R but are also mostly monoparametric with logP as the dominant descriptor. More informative from the standpoint of molecular interactions are QSRR models for cyano phase, and the quality of those models depends on the mobile phase modifier (the best was acetone and the worst propanol). It is suggested to consider extrapolated retention on cyano phase as alternative to standard RP-18 for further assessment of plasma protein binding, since all QSRR models use extrapolated retention as a property which is indirectly connected with plasma protein binding.

J. Chromatogr. 698, 351-359 (1995). Test of transfer efficiency from polyacrylamide gels and binding to Immobilon P and CD in different buffers with 125I-labelled proteins. Staining of Immobilon CD with toluidine blue and iodine vapor. Comparison of staining protocol on Immobilon CD. Discussion of structural analysis on blotted and stained proteins, and of the transfer conditions.

J. Chromatogr. A 773, 299-309 (1997). Protein purification by using a combination of molecular mass exclusion membranes with electrophoresis. Description of the method for fractionation of proteins from complex protein mixtures by reflux electrophoresis using the Gradiflow, which allows rapid method development for defining optimal conditions and shortening times, resulting in higher yields.

J. Chromatogr. A 1526, 157-166 (2017). Introduction of a promising alternative method for protein analysis using HPTLC with its high level of variability regarding the chromatographic system (multiple mobile and stationary phases, even mixed) and manifold detection as well as hyphenation possibilities. Silica gel, cellulose, and different RP layers were investigated with regard to their applicability for HPTLC-immunostaining. HPTLC of intact proteins on silica gel with 2-butanol – pyridine – ammonia – water 39:20:10:31; on cellulose with 2-butanol – pyridine – ammonia – water 32:30:11:25; and on RP phase with acetonitrile – trifluoroacetic acid – water 400:30:37. After development the plate was incubated with Tween20 as blocking reagent in a small vessel to inhibit unspecific binding of the antibodies to the surface. Then the plate was incubated for 2 h with the primary antibody solution and after washing with the secondary antibody for 1 h. Detection by incubating the plate in a dying solution containing 0.06% 3,3',5,5'-tetramethylbenzidine, 0.2% dioctyl sulfosuccinate sodium salt, 0.7% citric acid monohydrate, 1.8% sodium hydrogen phosphate dihydrate, 25% ethanol, and 1.5‰ dihydrogen dioxide, until blue zones appeared under white light. For the example analysis of beta-lactoglobulin on silica gel using antibovine beta-lactoglobulin antibodies, linearity was in the range of 75-2000 ng, the LOD was 62 ng/zone, the LOQ 93 ng/zone, and the accuracy 98.3%.