CLB Chemie in Labor und Biotechnik 47, 262-263 (1994). HPTLC screening of carcinogenic amines in textiles on silica with dichloromethane - acetonitrile 5:1. Derivatization by diazotation and coupling with N-(1-naphthyl)-ethylenediammonium dichloride. Visual evaluation (pink to blue zones on a pale background). For confirmation of positive results a 15 step AMD gradient on silica from 80% dichloromethane - acetone 1:1 and 20% hexane to 20% dichloromethane - acetone 1:1 and 80% hexane follows. Alternative chromatography on silica with diisopropyl ether - pentane - diethyl amine 12:8:1. Quantification by densitometry (absorbance) at 200, 230, 280 and 360 nm (multi-wavelength-scan). The detection limit of 20 derivatized amines is between 20-200 ng and of not derivatized between 20-400 ng. Sample preparation includes treatment with dithionite reagent, cleaving azo dyes into their component amines. Very effective screening method. Similar topic published: "Identification and determination of carcinogenic amines of the MAK value list III A1/A2." in Int. Lab. Nov., 29-30 (1996); and "Nachweis und Bestimmung verbotener Amine aus der Spaltung von Textil-Azofarbstoffen. (Identification and determination of banned amines derived from textil azo dyes.) in GIT Fachz. Lab. Spez. Chromatogr. 2, 92-94 (1995).

J. Planar Chromatogr. 14, 4-7 (2001). HPTLC of dansyl derivatives of polyamines (putrescine, cadaverine, spermidine, spermine) on silica gel with chloroform - triethylamine 2:1 containing 5% polyoxyethylene-10-lauryl ether (light protected). Quantitative analysis was achieved by in-situ fiberoptic-based fluorescence scanning at 338 and 502 nm. The compounds were determined over the range 0.5 - 85 ng, with relative standard deviations between 0.44-1.16% and detection limits in the range 0.28-0.39 ng. Rapid, precise, and versatile procedure.

Chromatographia 64 (7-8), 423-427 (2006). TLC of six polyamines (ornithine, citrulline, putrescine, cadaverine, spermidine and spermine) on calcium sulfate (CaSO4) and silica gel with 11 different mobile phases, using methanol as the solvent to enhance selectivity and produce differential Rf values. On CaSO4 better separation was achieved than on silica gel, no derivatization is needed, and development time is about 1/3 shorter. Quantitative determination by absorbance measurement at 550 nm after extracting substances from the plates and eluting from the coating material. Limit of detection (LOD) was 750 ng, limit of quantification (LOQ) was 1880 ng. Quantitative separation of underivatized polyamines in spiked human urine samples.

Asian J. Chem. 20(7), 5409 - 5413 (2008). HPTLC of atomoxetine HCl on silica gel with acetonitrile - acetic acid 9:1. Quantitative determination by absorbance measurement at 269 nm. The method was linear in the range of 100-1000 µg/mL. The recovery was 99.8 %. The method was suitable for routine analysis of atomoxetine HCl in its pharmaceutical preparations.

62nd Indian Pharmaceutical Congress Abstract No. F-253 (2010). TLC of ambroxol hydrochloride and doxophylline on silica gel with n-butanol – toluene – ethyl acetate – 25 % ammonia 50:30:20:1. The hRf values were 36 and 45 for doxophylline and ambroxol, respectively. Quantitative determination by absorbance measurement at 258 nm. The method was linear in the range of 300-1100 ng/band for ambroxol and 100-1100 ng/band for doxophylline.

Food Control. 68, 220-228 (2016). HPTLC of biogenic amines (putrescine, histamine, tyramine and agmatine) in microbial cultures on silica gel with chloroform – benzene – triethylamine 12:9:2. Quantitative determination by absorbance measurement at 254 nm.

J. of Medicinal Chemistry 32, 297-301 (1989). TLC of N- (dipeptidyl)-S-acetylcysteamine and N, N’- (dipeptyl)cystamine on silica. Detection under UV, exposure to iodine vapor or spraying with ninhydrin.

Talanta 38, 1303-1307 (1991). Use of o-phthalaldehyde in sulfuric acid solution as spray reagent in TLC of some tryptophan derived indole alkylamines on silica with ethyl acetate - propan-3-ol - conc. NH3 45:35:20. Detection limits, from 20 to 100 ng per spot. Application examples.