Marine Drugs 21(1), 2 (2023). Samples were methanol extracts of cultivated marine bacteria Thalassomonas actiniarum, T. viridans and T. haliotis (Colwelliaceae),  as well as cholesterol, cholic acid, and deoxycholic acid as standards. TLC on silica gel with n-hexane – ethyl acetate – methanol – acetic acid 20:20:5:2. After drying at room temperature, visualization by spraying with phosphomolybdic acid (10 % in ethanol) and heating with a heat-gun. For isolation of cholic acid (hRF 80), present in all samples, preparative TLC on silica gel with the same mobile phase, the corresponding band was scraped off with a surgical blade and extracted with methanol overnight. The isolated cholic acid was identified by LC-MS.

J. Planar Chromatogr. 33, 271-279 (2020). Two-dimensional HPTLC of cholic, ursodeoxycholic, chenodeoxycholic, deoxycholic, and lithocholic bile acids on silica gel with 0.0001 M cetylpyridinium chloride at pH 9 with the addition of aliphatic alcohol modifiers 4 % 1-butanol (direction II) and 0.6 % 1-pentanol (direction II). Detection by drying the plate for 2-3 min in the oven at 120 ºC, followed by spraying with 8 % sulfuric acid in ethanol. Qualitative determination under UV light at 365 nm.

J. AOAC Int. 102, 708-713 (2019). TLC of snake bile in 20 species from three families (Elapidae, Colubridae, and Viperidae) on silica gel with xylene – isopropanol – methanol – glacial acetic acid – water 80:40:30:20:3. Detection by spraying with a 10% sulfuric acid ethanol solution, followed by heating at 105 ºC.  Qualitative identification under UV light at 366 nm. TLC coupled with quadrupole–time-of-flight–MS analysis of each zone was used for compound identification and evaluation.

(Chinese). Bulletin Chinese Trad. Med. 12, 350-352 (1987) (Zhongyao Tongbao). TLC on silica with cyclohexane - ethyl acetate - acetic acid 2:8:1.5. Quantification by densitometry at 365 nm. Detection limit < 1 µg.

J. Chromatogr. A 811, 171-180 (1998). 2D-TLC of five major unconjugated bile acids, cholic acid, chenodeoxycholic acid, deoxycholic acid, ursodeoxycholic acid and lithocholic acid, and their conjugates on RP-18 with aqueous methanol in the 1st dimension and the same solvent system containing Me-b-CD in the 2nd. Detection by spraying with 20% phosphomolybdic acid in methanol and heating at 110°C. Application of this 2D inclusion RP-HPTLC method to the separation of glycine-conjugated bile acids in human bile.

J. Chinese Herb Med. 19, 332 (1988) (Zhongcaoyao). TLC of bile acids on silica with isooctane - ether - acetic acid - butanol - water 10:5:5:3:1. Detection by spraying with 30% sulfuric acid and heating at 105°C for 10 min. and observing under UV 365 nm. Fingerprint identification by comparing with standards.

I. J. Liq. Chromatogr. & Rel. Technol. 26, 1095-1108 (2003). TLC of 7 (cholic acid, chenodeoxycholic acid, deoxycholic acid, lithocholic acid, glycocholic acid, glycodeoxycholic acid, glycolithocholic acid) on silica gel with n-heptane - ethyl acetate -acetic acid 25:20:8. Evaluation with a 10% solution of sulfuric acid in water as visualizing reagent. The spots were developed by heating at 120°C for 20 min. The retardation factor, the separation factors, resolution factors, as well as the constants of pair separation indicated the mobile phase mentioned is the best of 8 mixtures investigated.

Collaborative study. J. Chromatogr. 452, 399-408 (1988). Comparison of quantitative analysis of bile acids in biological samples with other methods. Discussion of the specificity, sensitivity, accuracy and simplicity of each. TLC gives reliable results for biliary bile acids except for differentiation between conjugates dihydroxycholanoic acids. The method compared were enzymatic analysis, radioimmunoassay, GC, HPLC and GC/MS.