J. Planar Chromatogr. 16, 268-270 (2003). TLC of the Z and E diastereomers of the O-methyloximes of testosterone and 17a-methyltestosterone on silica gel with ether - petroleum ether (30-60°C) 1:1. The RF values of these compounds could be rationalized by a quantitative structure-property relationship (QSPR) using one topological/topographical index.

J. Chromatogr. 329, 171-177 (1985). Two-dimensional TLC of unconjugated metabolites of neutral steroids on silica with 1) 1,2-dichloroethane - methyl acetate 8:2 and 2) hexane -1-hexanol 65:35. Detection with Liebermann-Burchard reagent.

J. Planar Chromatogr. 2, 33-38 (1989). Two-dimensional TLC of anabolics on silica with 5 different solvent systems. Reference mixtures contained 2 - 20 ng of the steroids (zeranol, zearalenone, a-nortestosterone, medroxyprogesterone acetate, trenbolone, trebolone acetate). Alternatively a „4x4“ developing mode could be used. In this mode 4 samples are developed in two dimensions on one HPTLC plate. For inducing fluorescence reaction, the plates were dipped into a 5% sulfuric acid - ethanol solution for 30 s and then incubated at 95°C for 10 min. The fluorescence before and after heating was observed under UV 366 nm. The described method permit the routine detection of various anabolic residues in bovine urine at levels of 0.5 - 10 ppb. The recoveries are high (70 - 90%). Standard deviation were between 2 and 8%.

CBS 93, 10-12 (2004). HPTLC of progesterone on silica gel prewashed by development in AMD2 first with chloroform - methanol 1:1 and then with the mobile phase, followed by drying at 80 °C for 15 min. Development in AMD2 with toluene - 2-propanol 10:1 without preconditioning over 60 mm. Quantitative determination by absorbance measurement at 252 nm followed by spectra recording from 200 to 360 nm. The linear working range is 25.7-154.5 ng/zone. Repeatability (standard deviation calculated from the amounts of seven simulated progesterone samples determined on the same plate at three concentration levels in the lower, middle and upper range) is 0.26-1.29 %. Recovery is 99.88-100.97 %. Reproducibility was performed with recycled HPTLC plates.

Chem. (Bunseki Kagaku) 34, 612-618 (1985). (Japanese). (High-purity, high-recovery purification of prednisolone by the automatic recrystallization method and its purity evaluation by thin-layer chromatography and by differential scanning colorimetry.) TLC of prednisolone on silica with chloroform - methanol 9:1. Detection by UV 254 nm.

J. Chromatogr. 465, 448-450 (1989). Two-dimensional TLC of title compounds on silica with ethyl acetate - chloroform 2:1 for the 1st direction and benzene - chloroform - methanol - 5 M acetic acid 70:20:20:1 for the 2nd. Detection of the fluorescent spots under UV light.

J. Liq. Chromatogr. Relat. Technol. 29, 1891-1903 (2006). HPTLC of androsterone, epi-androsterone, dehydro-epi-androsterone, testosterone, stigmasterol, beta-sitosterol, estradiol, hydrocortisone, and cholesterol on RP-18 W with methanol - water, and acetonitrile - water in different composition, with chamber saturation. Detection by spraying with sulfuric acid - methanol 1:9 and heating at 120 °C for 15 min. Densitometric determination of RF values. The aim of the work was to compare the lipophilicity of selected steroids determined by RP-HPTLC on RP-18 W plates using different mobile phases with lipophilicity values estimated by computational methods.