PLoS ONE 18(10), e0292514 (2023). Samples were 8 glycolipids, either sialylated or not; the two main examples were monosialotetrahexosylganglioside (GM1) and gangliotetraosylceramide (= asialo-GM1 = ASGM1). TLC on silica gel with chloroform – methanol – water – acetic acid 300:200:30:1, followed by air-drying. Visualization of glycolipids by spraying 5-methylresorcinol solution (0.2 % in 2 M sulfuric acid), followed by 5 min heating at 110°C. For immunostaining, underivatized chromatograms were immersed into a blocking gel (1% gelatin, 1% polyvinylpyrrolidone and 1mM EDTA in phosphate-buffered saline solution (PBS)), followed by immersion into a solution of monoclonal (50 ng/mL) or polyclonal (1:1,000) anti-ASGM1 antibodies purposedly produced in rabbits. After 1h reaction at room temperature, the layers were washed thrice with T-PBS (0.05 % Tween 20 in PBS) and horseradish-peroxidase-conjugated anti-rabbit IgG (1:40,000) was added. After washing thrice with T-PBS, the TLC sheets were incubated in substrate solution (tetramethylbenzidine). Blue spots indicated the glycolipids bound by the antibody. In this TLC assay, each of the five monoclonal antibodies (as well as the polyclonal serum) was specific to ASGM1 (unsialylated), whereas in ELISA (enzyme-linked immunosorbent assay) at the same concentrations three of them displayed partial cross-reactivity towards GM1, GM3 or GD1b, which are sialylated glycolipids. 

J. Sep. Sci. 46, 2300368 (2023). Review of chromatographic methods for the analysis of polysaccharides, including TLC and HPTLC. The paper described general process and fully automated techniques, mobile phases and detection methods.

Biochem. System. and Ecology 12, 183-188 (1984). TLC on silica with ethyl acetate - isopropanol - water 65:22:11; visualization by spraying with 85 % phosphoric acid - acetic acid - aniline - diphenylamine 20 ml + 100 ml + 5 ml + 5 g and heating at 100 °C for 2-3 h. Identification of sugars by co-chromatography with authentic standards.

J. Agric. Food Chem. 49, 366-372 (2001). TLC of 2,3-O-isopropylidene-5-O-toluene-sulfonyl-D-ribono-1,4-lactone and 2,3-O-isopropylidene-5-iodo-D-ribono-1,4-lactone on silica gel with hexane - ethyl acetate 2:1. Visualization by spraying with iron(III)chloride - hydroxylamine reagent and with alkaline methanolic triphenyltetrazolium chloride solution for 2,3-O-isopropylidene-5-deoxy-D-ribose and 5-deoxy-D-ribose.

CBS 104, 13-15 (2010). HPTLC of aloeverose, glucose and galactose in aloe vera products on silica gel (impregnated with NaH2PO4) with acetone - iso-propanol - 0.1 M formic acid 2:2:1 (double development for products with high glucose content). Detection by immersion in 4-aminobenzoic acid reagent (1 g 4-aminobenzoic acid in 36 mL acetic acid, with 40 mL water, 2 mL 85 % phosphoric acid and 120 mL acetone), followed by heating at 100 °C for 10 min. Densitometric evaluation by fluorescence measurement at 366 nm. The limit of quantification was approx. 3 % in aloe vera products. RSD for polynomial calibration is 2.1 % for mannose in the range of 8-80 ng/band and 4.0 % using matrix calibration.

J. Chromatogr. 360, 397-406 (1986). TLC of 6-o-alpha-D-glucosyl-, 6-0-alpha-maltosyl and 6-o-alpha-maltotriosyl derivatives of alpha, beta- and gamma cyclodextrin (CD) and of 6,6'-di-o-alpha-D-glucocyl-beta-CD on 4 kinds of plates with different solvent systems. Chromatography on silica with 1-propanol - ethyl acetate - water, 25 % NH3 3:1:2:1, on NH2-bonded silica with acetonitrile - water 3:2, on Si 50000 silica with 1-butanol - pyridine - water 6:4:3. TLC is a more convenient method than HPLC for the analysis of CDS.

Anal. Chem. 73, 3804-3807 (2001). TLC of lipopolysaccharides from intact bacteria on silica gel with chloroform – methanol – water – triethylamine 12:6:1:0.04. Detection by spraying with 10 % sulphuric acid in ethanol followed by heating at 150 °C for 5 min. For the non-destructive visualization the plates were pre-washed twice with propanol – water 1:1 and developed in methanol – water 1:1 which made the products appear as dull white spots on a bright background. Zones containing lipopolysaccharides or lipid A molecular species were isolated from the plate and analyzed by MALDI mass spectrometery.

Bioorg. Chem. 38, 56-61 (2010). TLC of 4-methoxyphenyl glycoside on silica gel with acetonitrile - methanol - water 4:2:1. Detection by spraying with warm 2 % ceric sulphate in 2N sulfuric acid. The hRf of 4-methoxyphenyl glycoside was 30.