Rapid Commun. Mass Spectrom. 35, 9013 (2021). Review of the application of direct analysis in real time (DART) combined with mass spectrometry (MS) detection in food science and industry published in the period from 2005 to this date. The applications described the use of HPTLC in preparation methods as well as the combination of HPTLC fingerprints and DART-MS with multivariate data analysis for the differentiation of natural propolis products.

J. Ethnopharmacol. 276, 114144 (2021). Review of analytical methods for the analysis of the main secondary metabolites detected and isolated from Ammi majus. The paper described TLC and HPTLC methods for the determination of coumarins in fruits.

J. Ethnopharmacol. 281, 114503 (2021). Review of the reported phytochemical studies of Sesamum indicum, including TLC and HPTLC methods for the analysis of major compounds. 

J. Ethnopharmacol. 275, 114054 (2021). Review of methods and their conditions for quality control of herbal formulations derived from Andrographis paniculata by analytical techniques, including TLC and HPTLC. The document provided specific validation data for the analysis of andrographolide in different systems.

Phytochem. Anal. 3091 (2021). Comprehensive review of the application of chromatographic methods for the quality control of fenugreek seeds. The document described systems for the analysis of fenugreek, including the determination of trigonelline, 4-hydroxyisoleucine and diosgenin in seeds from different origins and in herbal formulations.

Food Control. 127, 108113 (2021). Review of various techniques used for adulterant authentication in spices and herbs, including identification of unusual profile of a component, differentiation from the normal standard compound and identification of marker or foreign material in the product. The document provided an overview of chromatographic methods, including TLC and HPTLC for adulterant detection, and its combination with mass spectrometry. 

J. Liq. Chromatogr. Relat. Technol. https://doi.org/10.1080/10826076.2021.1968429 (2021). Review of bioautography procedures of antimicrobial compounds in plants, including extraction of plant material, stationary and mobile phases in direct bioautography, chemical derivatization and biological detection of bioactive compounds and structure elucidation of bioactive bands. The paper also discussed new aspects of identification of biologically active substances in accordance with the principles of green analytical chemistry, and with special reference to substances with antimicrobial properties.

J. Liq. Chromatogr. Relat. Technol. 44, 148-170 (2021). Comprehensive review of the contribution of HPTLC to the analysis of lipids in the last ten years. Advances in detection, identification and determination of main classes of lipids and their subclasses separated by HPTLC in different matrices were discussed. The review described techniques for scanning densitometry of lipids through direct detection and on-plate staining using inorganic and organic agents, immunostaining of glycosphingolipids and labeled lipids. Techniques coupling HPTLC with mass spectrometry were also discussed, including extraction-based interfaces, ionization of lipids, on-plate matrix assisted laser desorption and ionization, and its application in the analysis of sphingolipids, neutral lipids and fatty acids and phospholipids.