J. Planar Chromatogr. 24, 316-319 (2011). HPTLC of Palmarosa oil extracts in toluene and geraniol on silica gel with toluene - ethyl acetate 37:3 in a twin-trough chamber saturated for 30 min at 25 +/- 2 °C. Detection by spraying with 3 % vanillin in ethanol - sulfuric acid 49:1 followed by heating at 100 °C for 5 min. Quantitative determination by densitometry in absorption mode at 400 nm. The instrumental precision and the repeatability (n = 6), was 0.3 and 3.2 %, respectively. LOD and LOQ was 1.4 and 2.8 µg/mL, respectively. The intra-day recovery was 99.8 % and the inter-day recovery 99.4 %. The hRf value for geraniol was 36.

Rev. Colomb. Cienc. Quim. Farm. 45, 147-168 (2016). HPTLC of propolis based lipid nanoparticles containing tea tree (Melaleuca alternifolia) on silica gel with toluene – ethyl acetate 93:7. Qualitative determination under UV light at 365 nm. Detection by spraying with anisaldehyde-sulfuric acid reagent (0.5 mL anisaldehyde was mixed with 10 mL glacial acetic acid, followed by 85 mL methanol and 5 mL concentrated sulfuric acid).

J. AOAC Int. 79, 628-635 (1996). TLC of allantoin in pharmaceuticals on silica with e.g. methanol - acetone - formic acid - water 40:2:1:6 and in urine on cellulose with butanol - acetic acid - water 2:1:1. Detection with pDMAB (Ehrlich's reagent). Quantification by densitometry at 445 nm. TLC as fast and inexpensive semiquantitative method for estimation of allantoin in natural samples.

J. Planar Chromatogr. 24, 154-159 (2011). TLC of avobenzone (methoxydibenzoylmethane), octocrylene, Uvinul T 150 (ethyl hexyl triazone, ET), and ethylparaben on silica gel with cyclohexane - diethyl ether 1:1 (method A), or with cyclohexane - piperidine 15:1 (method B), in a chamber lined with filter paper and saturated for 20 min. Quantitative determination by densitometry at 380 nm for avobenzene and at 300 nm for octocrylene. LOD and LOQ were 170 and 510 ng/zone for avobenzone (method A) and 180 and 537 ng/zone for octocrylene (method B), respectively. The linearity range was 200-1800 ng/zone for avobenzone and octocrylene.

CBS 113, 13-15 (2014). HPTLC of glycosylceramide Glc-d18:2 h16:0 from wheat germ and standards squalene, cholesteryl oleate, glyceryl trioleate, linoleic acid, ß-sitosterol, and ß-sitosterol glucoside on silica gel in the AMD 2 with a 18-step gradient modified from Opitz et al. (Chromatographia 73 (2011) 559), methanol replaced ethanol, and the mobile phase composition was changed slightly (pre-conditioning with 4 M acetic acid before each step, drying time 1.5 min, development duration 3 h and solvent consumption 200 mL). Detection by dipping in copper sulfate phosphoric acid reagent for 20 s and heating at 130 °C for 15 min revealed grey-brown bands. Densitometry evaluation by absorbance measurement at 546 nm. For Glc-d18:2 h16:0, regression analysis showed a polynomial relationship with coefficients of determination (R2) from 0.995 to 0.999 (n=3, 50 - 1000 ng/band). LOD (S/N 3) and LOQ (S/N 10) of Glc-d18:2_x000D_ h16:0 were 10 ng/band and 50 ng/band, respectively (n = 6).

J. Planar Chromatogr. 9, 185-188 (1996). HPTLC of buspirone, gepirone, ipsapirone, zalospirone and their resp. impurities on silica with the upper layer of n-butanol - acetic acid - water 4:1:2, isopropanol - butanone - NH3 50:50:1, or isopropanol - acetone - NH3 70:30:1; quantification by densitometry at 237 nm. Detection of a specific impurity by spraying with hydroxylamine solution. - Detection limits of a few nanograms were obtained at a signal-to-noise ratio 3:1. The relative standard deviation values for the main components and related impurities were between 2.2 and 3.4%.

J. Planar Chromatogr. 24, 227-231 (2011). TLC of diethylamino hydroxybenzoyl hexyl benzoate (DHHB) and octyl methoxycinnamate (OMC) on silica gel with cyclohexane - diethyl ether - acetone 15:1:2 with chamber saturation for 20 min. Quantitative determination by densitometry in absorbance mode at 300 nm (for OMC) and 360 nm (for DHHB). Linearity was between 200 and 2000 ng/zone both for DHHB and OMC. LOD and LOQ was 50 and 472 ng/zone for DHHB and 50 and 488 ng/zone for OMC. The hRf value was 30 for DHHB and 47 for OMC. Recovery of DHHB was between 97.8-101.9 % and of OMC between 98.8-102.7 %

CBS 115, 2-4 (2015). HPTLC of propolis tinctures and standards estrone, 17-estradiol, 17-ethinylestradiol, estriol, bisphenol A, 4-n-nonylphenol, and caffeic acid methyl ester on RP-18W with n-hexane – toluene – ethyl acetate 8:3:2 to a migration distance of 7 cm. For the HPTLC-pYES bioassay the plate was dipped into a yeast cell suspension and incubated for 3 h. Biodensitometric evaluation by fluorescence measurement at 365 nm with a cut-off filter of 400 nm. Elution of the bioactive zones with methanol – ammonium formate buffer (10 mM, pH 4, 49:1) into a ESI-MS. The LOD for 17-estradiol was 0.2 pg/zone and the LOQ 0.5 pg/zone. With this method estrogen effective substances can be detected with no or minimal sample preparation.