CBS 93, 2-4 (2004). HPTLC of signaling ceramides on silica gel with a 7 step gradient from methanol over dichloromethane to n-hexane over 42 min. Detection by dipping in manganese chloride reagent for 1 s, followed by drying at 120 °C for 20 min. Quantitative determination by absorbance measurement at 550 nm and Michaelis-Menten regression 2 via peak area. Signaling ceramides are separated from other lipids (shingomyelin, phosphatidylcholine, cholesterol) contained in cellular lipid extracts. Comparison with determination of ceramide formation via isotope labeled standards and conventional TLC method.

J. of Chromatogr. A 1218 (19), 2668-2675 (2011). Use of the changes in emission of berberine cation, induced by non-covalent interactions with lipids on silica gel for detection and quantification of lipids using fluorescence densitometry in HPTLC/AMD. Three different HPTLC/AMD gradients were developed for the separation of 1) neutral lipid families and steryl glycosides, 2) different sphingolipids, and 3) sphingosine–sphinganine mixtures. Rationalization of fluorescent molar responses of studied lipids, and differences in response among different lipid families in the light of a previously proposed model of FDIC response, which is based on ion-induced dipole interactions between the fluorophore and the analyte, likewise, application of computational calculations using molecular mechanics as a complementary useful tool to explain high FDIC responses of cholesteryl and steryl-derivatives, and moderate responses of sphingolipids. Proposal of an explanation for the high FDIC response of cholesterol, whose limit of detection is 5 ng.

J. Chromatogr. 408, 445-448 (1987). TLC of ecdysone, makisterone A, polypodine B, ponasterone A, ponasterone C and polypodine B 2-cinnamate on silica with various solvent systems based on hexane - dichloromethane and dichloromethane - methanol using AMD. Detection by visualizing under UV 254 nm; densitometry by absorbance at 260 nm. Comparison of the results with those from manual multiple development and single development.

J. Planar Chromatogr. 4, 424-431 (1991). TLC determination of poly(ethylene glycols) with a nominal molecular weight up to 1000 amu as 3,5-di-nitrobenzoates on silica. The increased spot capacity afforded by AMD and selective solvent systems was demonstrated to be crucial to the resolution of samples with a broad oligomer distribution.

J. Planar Chromatogr 8, 257-268 (1995). HPTLC of cinnamon and cassia oils and of solvent extracts of powdered cinnamon and cassia (i.a. cinnamic acid, cinnamyl alcohol, eugenol, coumarin, 2-methoxycinnamaldehyde, cinnamyl acetate) on silica with hexane - chloroform - triethylamine 45:3:2 or chloroform - hexane - methanol 59:39:2. HPTLC of vanilla extracts (5-(hydroxymethyl)-2-furfural, 4-hydroxybenzoic acid, 4-hydroxybenzaldehyde, vanillic acid, vanillin) on silica with AMD. Quantification by densitometry at 255 nm resp. 280 nm.

J. Chromatogr. A 791, 231-235 (1997). HPTLC on silica gel developed with AMD with an optimized gradient based on dichloromethane - methanol - hexane. Detection by spraying with chlorosulfonic acid and heating for 30 min at 110°C. Visualization under UV365 nm and daylight. Correlation of the molecular structures of forskolin derivatives to the migration distance by multiple correspondence analysis.

J. Planar Chromatogr. 17, 290-296 (2004). Description of a new AMD instrument. Its main advantages are very low cost both of construction and in use. In comparison with ascending development in conventional instruments, a laboratory-made horizontal sandwich chamber is used for development. With the help of a series of special accessories no obvious mobile phase remains in the distributor after each step thus saving a large amount of solvent. All the components of the instrument are easy to obtain, so the average worker in the laboratory could construct all the instrument except the control unit. An application of the instrument is described; the results obtained were satisfactory. Compared with the commercial instrument the main differences are 1) a horizontal sandwich chamber with funnel distributor is used as development chamber, 2) the most expensive component, a motor-driven valve, is omitted, 3) a micro air pump (normally used to supply oxygen for goldfish) is used to deliver mobile phase to the chamber. AMD separation of 13 dyes with first acetone - ethyl acetate 1:1 to compress the spots to slim bands, then seven steps with ethyl acetate - chloroform 4:21 to1:9 were completed; then seven steps with chloroform - cyclohexane 17:3 to 67:33. After these fifteen steps of AMD the mixture was separated into eighteen visible spots.

CBS 105, 10-12 (2010). HPTLC of stratum corneum lipids (ceramides, cholesterol, phosphatidylcholine, squalene, sphingomyelin etc.) on silica gel by automated multiple development with a 8-step gradient from methanol to hexane in the AMD2 with pre-conditioning with 4M acetic acid before step 6. Detection by immersion in copper(II)sulfate reagent followed by heating at 170 °C for 8 min. Quantitative determination by absorbance measurement at 600 nm. Phosphatidylcholine and sphingomyelin remain at the start position, all other substances are separated.