60th Indian Pharmaceutical Congress PA-215 (2008). HPTLC of ranitidine HCl and domperidone in combined dosage form on silica gel with ethyl acetate - methanol - ammonia 100:10:1 in a twin trough chamber saturated for 10 min. Quantitative determination by absorbance measurement at 285 nm. The method was linear in the range of 100-500 ng/µL for both compounds with a recovery of 102.5-100.8 %.

Abstract No. F-10, 61st IPC (2009). HPTLC of Embella ribes Churna formulation on silica gel with chloroform - ethyl acetate - formic acid 5:4:1 in a twin trough chamber. Densitometric measurement of embelin at 291 nm. The method was linear in the range of 600-1800 ng/band with recovery value of 99.1-101.2 %. The formulation was also analyzed by HPLC and results were found to be comparable.

Ind. J. Pharma. Sc. 72(4), 513-517 (2010). TLC on silica gel with dichloromethane - methanol - 25 % ammonia 95:10:3. Ofloxacin and ornidazole were well separated. Linearity was in the range of 20-100 ng/band for ofloxacin and 50-250 ng/band for ornidazole. Recovery was in the range of 99.3-100.5 %.

J. Chromatogr. A 1218 (19), 2754-2774 (2011). HPTLC for lipid analysis is particularly useful for smaller, apolar compounds and offers some advantages over HPLC. Description of stationary phases, solvent systems and detection methods for the individual lipid classes (cholesterol and its derivates, glycerides, sphingo- and glycolipids, phospholipids). In comparison with common staining methods the combination of HPTLC and mass spectrometric detection methods is a very powerful method to investigate the identities of the HPTLC zones in detail.

Acta Chromatographica 22 (2), 173-187 (2010), DOI:10.1556/AChrom.22.2010.2.2. HPTLC on silica gel with methanol – carbon tetrachloride – ethyl acetate – glacial acetic acid 80:636:280:4. The hRf values were 45 and 30 for atorvastatin calcium and losartan potassium, respectively. Quantification by densitometry at 238 nm. Linearity was in the range of 50–500 ng/band for each substance. The recoveries were 100.6 % and 100.5 % for atorvastatin calcium and losartan potassium, respectively. No interference from excipients was observed. The results were compared statistically using a paired t-test with results by an RP-HPLC method. Both methods provided comparable results.

Anal. Chim. Acta 632 (2), 168-180 (2009). Ochratoxins and aflatoxins are the most significant mycotoxins and there has been a broad range of research. However, it is impossible to use one standard technique for the analysis because of the various structures of mycotoxins. The review discusses existing analytical and detection techniques, such as 1) sample pre-treatment methods like liquid-liquid extraction, supercritical fluid extraction, or solid phase extraction; 2) separation methods such as TLC, HPLC, GC, and CE and 3) other methods such as ELISA. The practical requirements for high-sensitivity analysis and the need for a specialist laboratory setting create challenges for routine analysis. There are a number of methods used, but there is no single technique that stands out above the rest, although HPLC-MS is popular. Discussion of further currents trends, advantages and disadvantages and future prospects of these methods.

J. of China Pharm. 25 (8), 772-775 (2011). Safflower, the dried flower of Carthamus tinctorius L. is a herbal TCM drug for invigorating the circulation of blood, stimulating the menstrual flow, dissipating blood stasis, relieving pain, and is prescribed clinically to cure amenorrhea, falling injuries and skin and external diseases. Due to the lack of the source some counterfeits have been found on the market in recent years. The methods were studied for differentiating the dyes used by the market for adulteration of safflower. For dyes, TLC of the extracts of the crude drugs on silica gel firstly with chloroform – methanol – glacial acetic acid 7:1:2, detection under daylight for identification of orange, then with ethyl acetate – n-butanol – ethanol – ammonia – water 1:3:3:1:1, detection under daylight for identification of acid red 73, lemon yellow and carminum respectively. Results obtained by HPLC were compatible with those obtained by TLC.

J. Liq. Chromatogr. Relat. Technol. 36, 1323-1329 (2013). HPTLC of amlodipine (1) and perindopril (2) in bulk powder and tablets on silica gel with n-butanol - water - glacial acetic acid 4:5:1. Quantitative determination by absorbance measurement at 365 nm and 215 nm, for (1) and (2), respectively. The hRf values for (1) and (2) were 72 and 48, respectively. Linearity was 1-6 µg/mL for (1) and 2-10 µg/mL for (2). LOD and LOQ were 0.28 and 0.86 µg/mL for (1) and 0.24 and 0.75 µg/mL for (2), respectively. The interday and intra-day precisions were below 1.3 % (n=3). Recovery (by standard addition) was 98.0-99.6 % for both (1) and (2). Comparable results were obtained when compared with validated HPLC and first-derivative spectrophotometry methods, resulting in short scan time, large sample capacity, and use of minimal volume of solvent.