59th Indian Pharmaceutical congress C-305, 297, (2007). Phytoconstituents of mature and immature tubers of Ipomoea mauritiana (methanolic and aqueous extracts) have been studied. HPTLC on silica gel with chloroform - methanol - formic acid 6:3:1. Detection by spraying with vanilin - sulphuric acid reagent. Densitometric evaluation at 365 nm. Mature tubers were found to contain higher concentration of phytoconstiuents than immature tubers.

Indian Drugs 44 (12), 937-944 (2007). HPTLC of methanolic and ethyl acetate extracts of Sesamum indicum root on silica gel with ethyl acetate - n-hexane 1:9. Densitometric evaluation at 549 nm. The red zones were isolated by preparative TLC and identified by IR as 1,4 naphthoquinone derivatives.

using HPLC, HPTLC and densitometry. Phytochem. Anal. 19, 550-559 (2008). HPTLC of the leaves of Lawsonia inermis L., on silica gel with ethyl acetate – formic acid – water 82:9:9 followed by drying at 110 °C for 15 min. Detection by spraying with diphenylborinic acid aminoethylester 0.5 % in ethyl acetate, followed by drying and dipping into macrogol reagent (1 g polyethylene glycol 400 in 20 mL dichloromethane). Quantitative determination by absorbance measurement at 337 nm. Chemical fingerprint was used for quality evaluation of herbal products and detection of adulteration. Comparison with an HPLC method gave comparable results.

60th Indian Pharmaceutical Congress PA-215 (2008). HPTLC of ranitidine HCl and domperidone in combined dosage form on silica gel with ethyl acetate - methanol - ammonia 100:10:1 in a twin trough chamber saturated for 10 min. Quantitative determination by absorbance measurement at 285 nm. The method was linear in the range of 100-500 ng/µL for both compounds with a recovery of 102.5-100.8 %.

Abstract No. F-10, 61st IPC (2009). HPTLC of Embella ribes Churna formulation on silica gel with chloroform - ethyl acetate - formic acid 5:4:1 in a twin trough chamber. Densitometric measurement of embelin at 291 nm. The method was linear in the range of 600-1800 ng/band with recovery value of 99.1-101.2 %. The formulation was also analyzed by HPLC and results were found to be comparable.

Ind. J. Pharma. Sc. 72(4), 513-517 (2010). TLC on silica gel with dichloromethane - methanol - 25 % ammonia 95:10:3. Ofloxacin and ornidazole were well separated. Linearity was in the range of 20-100 ng/band for ofloxacin and 50-250 ng/band for ornidazole. Recovery was in the range of 99.3-100.5 %.

J. Chromatogr. A 1218 (19), 2754-2774 (2011). HPTLC for lipid analysis is particularly useful for smaller, apolar compounds and offers some advantages over HPLC. Description of stationary phases, solvent systems and detection methods for the individual lipid classes (cholesterol and its derivates, glycerides, sphingo- and glycolipids, phospholipids). In comparison with common staining methods the combination of HPTLC and mass spectrometric detection methods is a very powerful method to investigate the identities of the HPTLC zones in detail.

Acta Chromatographica 22 (2), 173-187 (2010), DOI:10.1556/AChrom.22.2010.2.2. HPTLC on silica gel with methanol – carbon tetrachloride – ethyl acetate – glacial acetic acid 80:636:280:4. The hRf values were 45 and 30 for atorvastatin calcium and losartan potassium, respectively. Quantification by densitometry at 238 nm. Linearity was in the range of 50–500 ng/band for each substance. The recoveries were 100.6 % and 100.5 % for atorvastatin calcium and losartan potassium, respectively. No interference from excipients was observed. The results were compared statistically using a paired t-test with results by an RP-HPLC method. Both methods provided comparable results.