J. of Qilu Med. & Pharm., 30 (2), 91-93 (2011). TLC of the extracts of Wuyangbuxin capsules 1) for Polygonium multiflorum, on silica gel with the upper phase of toluene - ethyl acetate - formic acid 10:1:1, detection under UV 365 nm; 2) for Licorice, on silica gel with petroleum ether (60-90 °C) - toluene - ethyl acetate - glacial acetic acid 20:40:14:1, detection by spraying with 10 % sulfuric acid in ethanol and heating until zones were detected; 3) for Milkvetch root, on silica gel with the lower phase of chloroform - methanol - water 13:6:2 at 10 °C, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until zones were detected; 4) for Epimedium herb on silica gel with ethyl acetate - butanone - formic acid - water 10:1:1:1, detection by spraying with AlCl3 reagent, heating at 105 °C until zones were detected and viewing under UV 365 nm. Identification by fingerprint comparison with the standards of the individual drug components.

J. Liq. Chromatogr. Relat. Technol. 35, 1141-1145 (2012). HPTLC of diosgenin in various parts of Tribulus terrestris L. on silica gel with toluene - ethyl acetate - methanol 7:3:1. Detection by dipping in anisaldehyde reagent consisting of anisaldehyde - acetic acid - sulfuric acid - methanol 1:20:10:170, followed by heating at 110 °C for 2 min. Quantitative determination by absorbance measurement at 430 nm. The hRf value of diosgenin was 48 and selectivity regarding matrix was given. Linearity was between 50 and 240 ng/zone. The method provides acceptable intra-day and inter-day precision for diosgenin. The limits of detection and quantification were 2 and 7 ng/spot, respectively. Recovery (by standard addition) was 100.6 %. The method provided comparable results with HPLC.

Food Control. 34, 138-148 (2013). TLC was reviewed as a fast screening methodology for the determination of deoxynivalenol (DON) showing LOD in rice of 100 mg/kg. 2D-TLC on silica gel with dichloromethane - ethyl acetate - propan-2-ol 18:1:1 and toluene - ethyl acetate - formic acid 6:3:1 for the first and second runs, respectively. Detection by spraying with chromotropic acid, followed by heating at 110 °C for 5 min. Quantitative determination by absorbance measurement at 366 nm. The hRf values of DON were 13 and 20. Advantages and disadvantages of current methods as well as comparison with other techniques were also described for the effective monitoring and surveillance of DON and its derivatives.

J. Liq. Chromatogr. Relat. Technol. 37, 2872-2881 (2014). 2D-HPTLC of 17 alpha-ethinylestradiol on RP-18 with methanol – acetonitrile – water 2:2:1 in the first direction and cyclohexane – butyl acetate – methanol 8:6:1 in the second direction, both over 70 mm. With the grafted method both plates were combined in a 2D separation. Detection by dipping into a mixture of sulfuric acid (98 %) and water (1:49), followed by heating at 110 °C for 10 min. Compound was also detected using a yeast estrogen screening assay using the strain Saccharomyces cerevisiae BJ3505.

J. Planar Chromatogr. 28, 268-273 (2015). HPTLC of astragaloside IV (1) and formononetin (2) in dried roots of Radix astragali (Astragalus membranaceus) on silica gel with petroleum ether - n-butanol saturated with water - glacial acetic acid 7:4:8. Detection by spraying with 10 % sulfuric acid ethanol solution, followed by heating at 105 °C. Quantitative determination by absorbance measurement at UV 254 nm. The hRF values for (1) and (2) were 43 and 75, respectively. Linearity was in the range of 1-10 μg/mL. The intermediate precision was below 0.4 % (n=6). Recovery ranged between 98 and 99 %. Compared to HPLC, HPTLC offered a rapid, economic, and sensitive method for detection of compounds.

J. Chromatogr. Sci. 56 (1), 92-98 (2018). TLC for direct enantiomeric resolution of the racemic β-adrenolytics bisoprolol (1), atenolol (2), propranolol (3), salbutamol (4) and carvedilol (5) by TLC using bovine serum albumin (BSA) as chiral additive in the stationary phase and with different compositions of simple organic solvents without buffer or inorganic ions. Systematic investigation of the effect of variation in pH, temperature, amount of BSA as the additive, and composition of mobile phase on resolution. Detection by exposure to iodine vapors. The five entantiomers were separated in the elution order (1), (2), (3), (4), and (5). The LOD was 0.7, 1.2, 0.8, 1.6 and 0.9 μg/zone for (1), (2), (3), (4), and (5), respectively.

Chem. (FENXI HUAXUE) 12, 455-458 (1984). (Chinese). (Studies on micellar solution as mobile phase in liquid chromatography. I. Use in thin-layer chromatographic separation of drugs). TLC of 33 drugs including sulfanilamides, vitamin B, antipyretics and adrenalines on polyamide with micellar aqueous solutions (SDS). Detection by various staining reagents and by UV.

Anal. Chem. 55, 2120-2126 (1983). TLC of long-chain alkylbenzemes on silica impregnated with 15 % AgNO3 with hexane. Location by visualization a standard band by spraying with 2',7'-dichlorofluorescein in methanol. Subsequent analysis of the eluate by GC. Comparison of TLC/GC technique with GC/MS.