Abstract GP-75, IPC (2005). HPTLC of satranidazole and its degradation products on silica gel with toluene - acetonitrile 3:2. For stability studies, the sample was treated with NaOH, HCl, H2O2 and photolysis. Degradation products were well resolved with significant different Rf values. The method had a linearity range of 100-500 ng. The proposed method suitable to investigate the kinetics of hydrolysis and photodegradation processes with first order in NaOH, and zero order for photolysis.

LC-GC Europe 14, Issue 10, 626-631 (2001). TLC of 20-hydroxyecdysone on silica gel, pre-washed with methanol. Different mobile phases are investigated, the mixture of chloroform — methanol — benzene 25:5:3 was used for quantification. Densitometry at 254 nm. TLC of ecdysteroids under the same conditions, detection at 254 nm, in white light and at 366/>400 nm (fluorescence) after spraying with a vanillin-sulphuric acid reagent. Identification was facilitated with two-dimensional TLC, displacement TLC (D-TLC) and forced flow TLC (FF-TLC). Comparison with HPLC.

J. Sep. Sci. 30, 772-777 (2007). HPTLC of haloperidol in tablets on silica gel with acetone – chloroform – n-butanol – acetic acid – water 2:4:4:1:1. Quantitative determination by absorbance measurement at 254 nm. Linearity was between 10 and 100 ng/µL, detection limit was 0.89 ng/µL, and the quantification limit was 2.71 ng/µL. Coefficient of variation is 2.35% and 4.50% for precision and accuracy, respectively. Successful comparison with HPLC measurements.

J. Chromatogr. A 1173 (1-2), 146-150 (2007). HPTLC of oenothein B and quercetin glucuronide in aqueous extract of Epilobii angustifolii herba on RP-18 W phase with 1) 25 % acetonitrile in water (with 50 mM H3PO4) over 80 mm for oenothein B, and 2) with acetonitrile over 40 mm for quercetin glucuronide. Quantification of oenothein B and quercetin glucuronide by densitometry at 270 and 350 nm, respectively. Linearity was between 1.14 and 2.28 µg/zone for oenothein B and 77 and 691 ng/zone for quercetin glucuronide. Aqueous extract of Epilobii angustifolii herba contained 152.46 ± 4.92 mg/g oenothein B and 22.07 ± 1.38 mg/g quercetin glucuronide.

Phytochem. Anal. 19, 353-358 (2008). HPTLC of galanthamine in the bulbs of Narcissus cv. on silica gel with chloroform - methanol 9:1 with chamber saturation for 30 min. Quantitative determination by absorbance measurement at 288 nm. The hRf value was 72 and selectivity regarding matrix was given. Linearity was in the range of 0.25 and 7.5 µg/spot. The limits of detection and quantification were 47 and 142 ng/spot, respectively. Recovery was 97.8 %. No significant intra- and interday variation was observed. The method proved to be precise, accurate, and rapid in comparison with a reference HPLC method.

Indian Drugs 44(12), 937-944 (2007). HPTLC of the methanolic extract of Sesamum indicum root (Pedaliaceae) and its ethyl acetate fraction on silica gel with ethyl acetate - n-hexane 1:9. Absorbance measurement at 549 nm. The red zone was isolated by preparative TLC and identified by IR to be a 1,4-naphthoquinone derivative.

Abstract No. F-315, 61st IPC (2009). HPTLC for pravastatin on silica gel with ethyl acetate - toluene - acetonitrile - formic acid 60:35:5:2. Quantitative determination by absorbance measurement at 237 nm. The method was linear in the range of 318-3816 ng/band. Recovery was 99.9-101.2 %. Stability tests showed that degradation products resulting under acid stress conditions were well resolved from the main component.

Abstract No. 259, 61st IPC (2009). HPTLC of quercetin in alcoholic extracts of the leaves of Cinnamomum malabatrum on silica gel with toluene - acetone - formic acid 36:12:5. Quantitative absorbance measurement at 254 nm. The alcoholic extract was found to contain 10.02 mg/g of quercetin. The total flavonoid content was estimated using a colorimetric method with aluminum chloride. Results were in good correlation with the HPTLC method.