Biomed. Chromatogr. 10, 256-260 (1996). TLC on silica impregnated with EDTA in different concentrations with 1) propionic acid - 2-propanol - water 2:1:1, 2) 2-propanol - water - ethyl acetate 5:3:3, 3) butanol - water - acetic acid 5:4:2. Detection by exposure to iodine vapor. Discussion of the effects of the concentration of the impregnating reagent and the composition of the mobile phase on the resolution.

J. Pharm. Biomed. Anal. 36, 33-41 (2004). A sensitive selective, precise and robust HPTLC method for the analysis of E and Z stereoisomers of guggulsterone (the hypolipidemic agent in the gum-resin exudates of Commiphora mukul) both as a bulk drug and in formulations was developed and validated. Separation on silica gel with toluene - acetone 9:1. Quantitative determination by absorbance measurement at 250 nm. This system was found to give compact spots for E- and Z-guggulsterone (Rf value of 0.38 ± 0.02 and 0.46 ± 0.02 respectively) following double development with the same mobile phase. Both E- and Z-guggulsterone showed good linearity in the concentration range of 100-6000 ng/spot. The method was validated for precision, robustness, recovery, and specificity. The proposed HPTLC method is suitable for the identification and quantitation of these isomers in herbal extracts and pharmaceutical dosage forms.

IPC 56th 2004, Abstract No. G-20. Simultaneous HPTLC determination of loratadine and ambroxol in combined dosage form on silica gel with n-hexane - dichloromethane - triethanolamine 11:8:1.The Rf value of loratadine and ambroxal was found to be 0.40 and 0.16 respectively. Quantitative evaluation by scanning at 254 nm.The method was linear in the range of 0.2 -1 mg/spot for loratadine and 1.2 - 6 mg/spot for ambroxol with recovery of 98.2 - 98.5 %. The method was validated for accuracy, precision, linearity, specificity, LOD, and LOQ.

J. Pharm. Biomed. Anal. 37, 817-821 (2005). CO2 extracts of Harpagophytum procumbens root was evaluated by HPLC and HPTLC for harpagoside contents. HPTLC on silica gel with ethyl acetate - methanol - water 77:15:8 in saturated ADC chamber. Detection by dipping into anisaldehyde reagent followed by drying at 120 °C for 5 min. Quantitative determination by absorbance measurement at 509 nm. The linearity range was 0.04-0.40 mg/mL. The HPTLC method was less time consuming than HPLC, needing almost no sample pre-treatment. 15 different CO2-extracts of the plant were analysed.

Indian Drugs 42 (6), 345-352 (2005). TLC fingerprint identification of methanolic extracts of Adhatoda vasica, Lawsonia inermis and Alkanna tinctoria, on silica gel. For Lawsonia inermis and Alkanna tinctoria the developing solvent toluene - acetone - acetic acid 90:10:1 was used, and for Adhatoda vasica n-hexane - acetone - diethyl ether 3:1:1 for. Detection of alkannin by spraying with 10 % methanolic KOH.

Chromatographia 64 (7-8), 423-427 (2006). TLC of six polyamines (ornithine, citrulline, putrescine, cadaverine, spermidine and spermine) on calcium sulfate (CaSO4) and silica gel with 11 different mobile phases, using methanol as the solvent to enhance selectivity and produce differential Rf values. On CaSO4 better separation was achieved than on silica gel, no derivatization is needed, and development time is about 1/3 shorter. Quantitative determination by absorbance measurement at 550 nm after extracting substances from the plates and eluting from the coating material. Limit of detection (LOD) was 750 ng, limit of quantification (LOQ) was 1880 ng. Quantitative separation of underivatized polyamines in spiked human urine samples.

J. Sep. Sci. 30, 708-712 (2007). HPTLC of icariin in the aerial part of Epimedium koreanum Nakai on silica gel with ethyl acetate – glacial acetic acid – formic acid – water 10:1:1:2. Quantitative determination by absorbance measurement at 270 nm. The LOD and LOQ for icariin were 66 and 215 ng/band, respectively. Results did not show statistical significance between HPLC and HPTLC.

59th Indian Pharmaceutical congress C-305, 297, (2007). Phytoconstituents of mature and immature tubers of Ipomoea mauritiana (methanolic and aqueous extracts) have been studied. HPTLC on silica gel with chloroform - methanol - formic acid 6:3:1. Detection by spraying with vanilin - sulphuric acid reagent. Densitometric evaluation at 365 nm. Mature tubers were found to contain higher concentration of phytoconstiuents than immature tubers.