J. Planar Chromatogr. 26, 486-490 (2013). HPTLC of tallow in cow ghee on silica gel with n-hexane - diethyl ether 2:3 + 1 drop glacial acetic acid for unsaponifiable fraction (1) and n-hexane - diethyl ether 13:7 + 1 drop glacial acetic acid for saponifiable fraction (2). Detection by spraying with 10% methanolic sulfuric acid reagent followed by heating at 110 ºC for 5-10 min. Zones at hRf values of 9 and 20 were identified as tallow concentration increased.

CBS 112, 2-4 (2014). TLC and HPTLC of chamomile (Matricaria recutita L.) flowers and standards herniarin, umbelliferone, alpha-bisabolol, and spiroethers on silica gel with chloroform - acetone 99:1 or dichloromethane up to 90 mm (TLC) and 75 mm (HPTLC). Detection under UV 254 and 366 nm and after dipping in vanillin reagent (0.4 % ethanolic vanillin solution with 2 % sulfuric acid) or DPPH radical reagent (0.02 % methanolic 2,2-diphenyl-1-picrylhydrazyl) for information on radical scavenging activity. Biological detection by dipping individually in a suspension of Bacillus subtilis, Aliivibrio fischeri, Pseudomonas syringae pv. maculicola and Xanthomonas vesicatoria. The essential oil component chamazulene showed the strongest antioxidative capacity.

extracts. J. Planar Chromatogr. 28, 178-183 (2015). TLC of (1) chlorogenic acid and (2) caffeine in coffee samples on silica gel with ethyl acetate - methanol - water 77:13:10 to a distance of 8 cm using a horizontal sandwich chamber. The plates were dried at room temperature for 1 h. Detection of polyphenols with NP-PEG reagent, of terpenoids with anisaldehyde-sulfuric acid reagent followed by heating at 110 °C for 5 min, of purine derivatives with iodine-hydrochloric acid reagent. Quantitative determination of (1) and (2) at UV 254 nm using VideoScan software. The hRF value of (1) was 14, and of (2) 51. The antibacterial activity of green and roasted coffee seeds and pomace was evaluated against Bacillus subtilis using TLC-direct bioautography. TLC-2,2-diphenyl-1-picrylhydrazyl (DPPH) test was used to determine the antioxidant properties. For bioautography with Bacillus subtilis, the plates were immersed for 8 s in the bacterial suspension, placed in a moistened plastic box, and incubated at 37 °C for 17 h. Detection after spraying with 0.2 % MTT aqueous solution. For the DPPH test, plates were sprayed with 0.2 % methanolic DPPH solution. Detection of antioxidant activities as yellow zones against a purple background.

Food Control. 68, 310-329 (2016). Review of the methodologies to determine the occurrence of aflatoxin M1 (AFM1) and the fate of AFM1 during processing of milk and dairy products, such as yoghurt and cheeses, since 1996 until today. The review describes the application of TLC and HPTLC in raw and pasteurized milk, feta cheese, yoghurt, white cheese, ice cream and butter.

J. Liq. Chromatogr. Relat. Technol. 39, 312-321 (2016). HPTLC of lupeol in waxes of different cabbage varieties on RP-18 with ethyl acetate – acetonitrile 5:1. Detection by dipping into the anisaldehyde reagent (16 mL sulfuric acid were dropwise added to a cooled mixture of 20 mL glacial acetic acid and 170 mL methanol, then 1 mL of anisaldehyde was added), followed by heating at 110 °C for 30 s. Quantitative determination by absorbance measurement at 535 nm. The hRF of lupeol was 40. Linearity was in the range of 30-150 ng/zone. The LOD and LOQ were 7 and 20 ng/zone, respectively. Intermediate precisions were below 10 %. Recoveries were between 90.3 and 93.1 %.

J. Food Comp. Anal. 52, 1-8 (2016). HPTLC of carbohydrates in maple sap, maple syrups and other_x000D_
natural sweeteners (corn syrup, sugarcane syrup, honey and agave syrup) on silica gel with butanol – propanol – water 3:12:4. Detection by spraying with aniline-diphenylamine phosphoric acid reagent in acetone. The hRF values for xylose, glucose, fructose, sucrose, maltose, kestose, isomaltose, maltotriose, nystose, maltotetraose, isomaltotriose, maltopentose, maltohexaose and maltoheptaose were 75, 64, 64, 60, 53, 46, 45, 41, 39, 30, 25, 21, 15 and 10, respectively.

J. Chil. Chem. Soc. 62, 3435-3437 (2017). HPTLC of deoxynivalenol in wheat crop samples on silica gel with toluene – ethyl acetate – formic acid 6:3:1. Detection by spraying with 10 % aluminium chloride in ethanol – water 1:1, followed by heating at 120 °C for 10 min. Quantitative determination by fluorescence measurement at UV 366/>400 nm. The identity and purity of zones were confirmed by direct mass spectrometry on the plate using a TLC/MS elution head-based interface. Linearity was between 8-120 ng/zone. LOD and LOQ were 0.05 and 0.19 mg/kg, respectively. Average recovery rate was 90.1 %.

J. Chromatogr. A 1525, 173-280 (2017). Presentation of a straightforward, selective and sensitive screening method for the determination of 16-O-methylcafestol (16-OMC) in roasted coffee, the characteristic diterpene exclusively present in Coffea canephora which is an excellent marker for Coffea canephora admixtures to Coffea arabica. HPTLC with fluorescence detection (HPTLC–FLD), using sudan IV as internal standard and a direct saponification with 10 % ethanolic potassium hydroxide solution, followed by solid-supported liquid extraction with petroleum ether. Selective derivatization of 16-OMC with 2-naphthoyl chloride and analysis by HPTLC–FLD on silica gel with cyclohexane – t-butyl methyl ether – formic acid 43:7:1. Quantitative determination of the enhanced fluorescence at 244/>320 nm. The LOD and LOQ was 5 and 14 mg/kg of 16-OMC in coffee, which allowed for the determination of Coffea canephora admixtures to Coffea arabica below 1 %. The recovery for blends of Coffea arabica with Coffea canephora was close to 100 %._x000D_