ex O.Berg) for its major anthocyanins by densitometry. J. Planar Chromatogr. 26, 363-369 (2013). HPTLC of delphinidin-3,5-diglucoside (1), petunidin-3,5-diglucoside (2), and malvidin-3,5-diglucoside (3) in the fruits of Eugenia jambolana on silica gel with ethyl acetate - formic acid - acetic acid - water - methanol 126:19:10:32:12. Quantification by absorbance measurement at 529 nm. The hRf values for (1), (2) and (3) were 17, 24 and 34, respectively. Linearity was in the range of 400-1000 ng/zone for (1) and (2) and 200-500 ng/zone for (3). LOD and LOQ were 84 and 283 ng/zone for (1), 72 and 242 ng/zone for (2) and 43 and 154 ng/zone for (3), respectively. Average recoveries were 95.4 % for (1), 96.3 % for (2) and 97.3 % for (3). Intermediate/interday/intra-day precision was below 5 % (n=3).

J. Planar Chromatogr. 27, 113-119 (2014). HPTLC of sulphadiazine sodium (1) and trimethoprim (2) in medicated fish feed, fish tissues, and in their veterinary pharmaceutical formulation on silica gel with chloroform - toluene - ethanol - glacial acetic acid 9:9:2:2. Quantitative determination by absorbance measurement at 270 nm. The hRF values for (1) and (2) were 48 and 16, respectively. Linearity was in the range of 100-2000 ng/zone for (1) and 100-1000 ng/zone for (2). The intermediate/interday/intra-day precisions were below 3 % (n=3). Mean recoveries for (1) and (2) were 99.6 and 97.9 %, respectively.

Food Addit. Contam. 31, 1929-1938 (2014). TLC of stearoyl-lactylates in food on silica gel with hexane - diethyl ether 1:1 and 1 % acetic acid. Detection by spraying with 0.05 % primuline solution in acetone - water 4:1. Qualitative identification at UV 366 nm.

(Rutaceae). Rapid Commun. Mass Spectrom. 29, 1530-1534 (2015). HPTLC of complex phytoconstituents in the peels of Citrus aurantium on silica gel with ethyl acetate - acetic acid - formic acid - water 100:5:5:13. Detection by spraying with vanillin reagent. Images were acquired by desorption electrospray ionization mass spectrometry in the positive ionization mode. The method allowed the identification of 20 compounds.

Food Chem. 210, 613-622 (2016). HPTLC fingerprinting of triterpenoids in Siam and Sumatra benzoin balsams on silica gel with n-hexane – methanol – acetic acid 8:2:1. Detection by dipping into anisaldehyde sulfuric acid reagent for 1 s (10 mL of sulfuric acid were carefully added to an ice-cold solution of 170 mL methanol and 20 mL acetic acid, followed by the addition of 1 mL of anisaldehyde (p-methoxybenzaldehyde)), followed by heating at 105 °C for 5 min. Qualitative identification under UV 366 nm. Two specific compounds at approximately hRF 5 (violet band) and 50 (beige band) were detected in the Sumatra sample. Siam benzoin is characterized by two specific compounds at approximately hRF 5 and 10 (brown bands) and two others at approximately hRF 20 and 60 (orange bands).

J. Liq. Chromatogr. Relat. Technol. 39, 607-612 (2016). HPTLC of glucose, fructose, xylose, rhamnose, arabinose, and galacturonic acid in Adansonia digitata dry fruit pulp on diol phase with a 15-step gradient based on acetone - acetonitrile 1:1 and water mixtures for enzymatic degradation products. Monosaccharide moieties were separated on silica gel with acetonitrile - acetic acid - water 63:33:5 to a developing distance of 80 mm. Detection by dipping into 10 % sulfuric acid in ethanol. Quantitative determination by absorbance measurement at 400 nm. The hRF values for rhamnose and glucose were 69 and 52, respectively. The HPTLC metehod could rapidly analyze complex mixtures containing a broad variety of monosaccharides that overlapped in a HPLC method.

Food Chem. 233, 290-301 (2017). Review of analytical methods for the effective control of patulin contamination, including TLC validated methodologies. The paper included a reference of standard methods, including the official AOAC method where detection is achieved by spraying with 3-methyl-2-benzothiazolinone hydrazone with a limit of detection of approximately 20 mg/L.

J. Chromatogr. A 1506, 109-119 (2017). HPTLC of seven important steviol glycosides on silica gel, which may degrade in food products under certain processing and storage conditions, and additionally as a sum parameter their reported breakdown products steviol and isosteviol. Detection with 2-naphthol and primuline reagent. Baseline separation of steviol and isosteviol was achieved after a plate cut and subsequent short development (two-step method). The HPTLC method was robust with regard to varying sample matrix loads, provided a high sample throughput (23 separations in parallel on one plate), and was fast (total analysis time of 1 h: 30 min application, 15 min separation and 15 min derivatization/densitometry, leading to 2.6 min per sample). The solvent consumption was low (0.4 mL per analysis) and accuracy of the densitometric quantification was good. Confirmation of the results with HPTLC-ESI-MS of only the zones of interest instead of matrix or background so that there was less need for MS cleaning. Hyphenation to Aliivibrio fischeri bioassay to obtain information on bioactive compounds in Stevia leaf extracts.