Food Chem. 121, 185-190 (2010). TLC of arbutin in the leaves and flowers of Origanum majorana and Origanum vulgare on silica gel with ethyl acetate - methanol - water 77:13:10. Detection by spraying with 2.5 % dibromchinochlorimide solution in ethanol. The hRf of arbutin was 47.

Food Research International 44, 1088-1093 (2011). HPTLC of resveratrol (1) and the resveratrol complex with hydrogenated soy phosphatidyl choline (2) on silica gel with dichloromethane – methanol 4:1. The hRf values of (1) and (2) were 87 and 92, respectively.

J. Planar Chromatogr. 24, 373-375 (2011). HPTLC of red clover capsule extracts and formononetin, biochanin A, daidzein, glycitein, and genistein on silica gel, prewashed with methanol, with dichloromethane - glacial acetic acid - ethyl acetate 12:2:1 in a horizontal chamber saturated for 15 min. Quantitative determination by densitometry at 260 nm. The hRf value was 29, 34, 41, 48, and 59 for daidzein, glycitein, genistein, formononetin, and biochanin A, respectively. The two major isoflavones are formononetin and biochanin A. The limit of detection and quantification was 14 and 47 ng/band for formononetin and 12 and 40 ng per band for biochanin A, respectively. The recovery was 93.3-100.7 % for formononetin and 102.0-109.4 % for biochanin A.

Chinese J. of Guangdong Agr. Sci. (9), 103-105 (2012). Garlic (Allium sativum L.) contains high levels of carbohydrates (up to 75 %), the main of which are fructo oligosaccharides (FOS). FOS have antioxidative activity. The quality control of garlic has to monitor the content of FOS because it changes during its storage. TLC of the extracts of garlic on silica gel twice with n-butanol – isopropanol – water – acetic acid 7:5:4:2, detection by spraying with vanillin – sulfuric acid – ethanol – water 3:13:81:26 and heating at 100 °C for 5 min. Up to seven monosaccharides in garlic are well separated by using the procedure.

Chinese Bulletin of Biol. 47 (7), 44-46 (2012). Chloroplast pigments play an important role in plant photosynthesis and become a kind of main raw material widely applied in the preparation of antitumor photodynamic drugs and functional foods. Phytoxanthin has outstanding function in vision protection, reducing the incidence of cataracts, anticancer therapy, and delaying the occurrence of arteriosclerosis. As a demonstration experiment for high school students analysis of chloroplast pigments by TLC and column chromatography. TLC of the extracts of fresh spinach leaves on silica gel with petroleum ether (30-60 °C) – benzene – acetone 7:1:5, detection in daylight, identification of beta-carotene (orange), chlorophyll a (dark green), chlorophyll b (light green) and phytoxanthin (bright yellow) by fingerprint comparison with the standards. Preparation by column chromatography on silica gel eluted with the same sovent system, collection of fractions, and the pure products were obtained by solvent evaporation. The results indicate that the TLC method is simple, sensitive, easy to operate, more intuitive, robust and more suitable for the purpose.

J. Liq. Chromatogr. Relat. Technol. 37, 1644-1653 (2014). HPTLC fingerprinting of 12 commercially available fruit teas on silica gel with n-butanol - formic acid - water 12:3:4:5. Detection by heating at 100 °C for 3 min, followed by dipping into NP reagent (1 g of diphenylborinic acid aminoethylester dissolved in 200 mL of ethyl acetate) followed by dipping into PEG reagent (10 g of polyethylene glycol 400 in 200 mL of dichloromethane). Quantitative determination by absorbance measurement at 366 nm. A principal component analysis allowed to distinguish between red fruit teas based on the chromatographic profile.

Innov. Food Sci. Emerg. Technol. 26, 490-497 (2014). HPTLC of the isoflavones (1) glycitin, (2) daidzin and (3) genistin in fermented soybean powder on silica gel with toluene - ethyl acetate - formic acid - acetic acid 2:16:2:1. Quantitative determination by absorbance measurement at 260 nm. The hRF values of (1) to (3) were 36, 44 and 58, respectively.

Innov. Food Sci. Emerg. Technol. 32, 127-135 (2015). HPTLC of prebiotic oligosaccharides produced in the orange juice on silica gel with acetonitrile - ethyl acetate - 1-propanol - water 17:4:10:18. Detection by spraying with 0.3 % 1-naphthyl ethylenediamine dihydrochloride in methanol with sulfuric acid 3 %, followed by heating at 120 °C for 10 min. The method was applied to determine the degree of polymerization. _x000D_