Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

  • Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
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      105 042
      Photometric procedure for quantitative analysis of aflatoxin B1 in peanuts by thin-layer chromatography using charge coupled device detector
      Michele HOELTZ*, J. WELKE, I. NOLL (*Instituto de Ciência e Tecnologia de Alimentos, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9500, 91570-901 Porto Alegre - RS, Brasil, michelehoeltz@yahoo.com.br)

      Quim. Nova 33, 43-47 (2010). HPTLC of aflatoxin B1 in peanuts on silica gel with chloroform - acetone 99:1. Quantitative determination by absorbance measurement at 366 nm, using a CCD camera followed by image processing using the software ImageJ. Linearity was between 0.8 and 4.8 ng/zone. The intra-day and inter-day precisions had a RSD lower than 5.2 %. LOD was 0.4 ng/zone while LOQ was 1.2 µg/kg. The average recovery was 94.9 %. The proposed method is a simple, efficient and low cost tool for quantitative analysis of aflatoxin B1 in peanut samples.

      Classification: 28b
      106 108
      Simultaneous determination of methylxanthines in different types of tea by a newly developed and validated TLC method
      Claudia CIMPOIU*, A. HOSU, L. SESERMAN, M. SANDRU, V. MICLAUS (*Babes-Bolyai University, Faculty of Chemistry and Chemical Engineering, 11 Arany Janos, 400028 Cluj-Napoca, Romania, ccimpoiu@chem.ubbcluj.ro)

      J. Sep. Sci. 33, 3794-3799 (2010). HPTLC of caffeine (1), theobromine (2) and theophylline (3) in different types of tea on silica gel with chloroform - dichloromethane - isopropanol 4:2:1. Quantitative determination by absorbance measurement at 254 nm. The hRF of (1), (2) and (3) was 65, 45 and 56, respectively. Limits of detection and quantification were 22 and 45 ng for (1), 23 and 46 ng for (2) and 22 and 43 ng for (3), respectively. The intra-day and inter-day precisions had a %RSD lower than 2.55 % (n=6) for all substances. Recoveries (by standard addition) were between 95.1-101.5 % for all the three methylxanthine derivatives. The values of LOD and LOQ obtained are similar with those obtained by HPLC.

      Classification: 21a
      108 030
      Fast quantification of 5-hydroxymethylfurfural in honey
      Elena CHERNETSOVA, Gertrud MORLOCK* (*Institute of Food Chemistry, University of Hohenheim, Garbenstrasse 28, 70599 Stuttgart, Germany, gerda.morlock@uni-hohenheim.de)

      CBS 107, 13-15 (2011). HPTLC of 5-hydroxymethylfurfural (HMF) in honey on silica gel, prewashed with methanol - water 6:1, with ethyl acetate. Quantitative determination by densitometry in absorbance mode at 290 nm. Optional detection by immersion in p-aminobenzoic acid reagent followed by heating at 110 °C for 5-10 min. The hRf value of HMF was 80. The calibration function was polynomial in the range of 0.8-80 ng/band whilst Michaelis Menten 2 regression was suitable for higher concentrations. The LOD of HMF in honey samples was 0.75 mg/kg (12 µL applied) and the LOQ 2.4 mg/kg. The method complies with the requirement of max. 15 mg/kg of HMF in honey. The results with this method were compared with those obtained by the spectrophotometric Winkler method and by HPLC-UV and mean differences were minor (3.3 % or 0.9 mg/kg). Complementary confirmation by HPTLC-MS online coupling. HMF zones identified under UV were eluted and analyzed by ESI-MS in full-scan and SIM mode.

      Classification: 8b
      110 026
      Crucial aspects of high-performance thin-layer chromatography quantitative validation
      Silvia CORAN*, S. MULAS, Nadia MULINACCI (*Departimento Scienze Farmaceutiche, Universita di Firence, Via Ugo Schiff 6, 50019 Sesto Fiorentino, Firenze, Italy)

      The case of determination of rosmarinic acid in different matrices. J. Chromatogr. A 1220, 156-161 (2012). Description of a new method for determination of rosmarinic acid in different matrices by HPTLC on silica gel with toluene – ethyl formate – formic acid 6:4:1. Quantification by densitometry in absorbance mode at 330 nm. The influence of the main HPTLC operative parameters was figured out in view of a more stringent validation process. Together with the fundamental HPTLC instrumentation an automatic developing chamber is mandatory as it allows for control of the relative humidity and the saturation conditions and thus assures reproducibility. Several commercial preparations containing rosmarinic acid in different amounts were tested and rosmarinic acid in the range of 132-660 ng/band was found. The %RSD of repeatability and intermediate precision did not exceed 2.

      Classification: 7
      111 023
      Application of a newly developed and validated high-performance thin-layer chromatographic method to control honey adulteration
      ANITTA PUSCAS, ANAMARIA HOSU, CLAUDIA CIMPOIU* (*Babes-Bolyai Univ., Faculty of Chem. & Chem. Engin., 11 Arany Janos, 400028 Cluj-Napoca, Romania)

      J. Chromatogr. A 1272, 132-135 (2013). Honey is a saturated solution of sugars, used for a long time as a natural source of sugars and is an important ingredient in traditional medicine due to its antimicrobial, anti-inflammatory and antioxidant effects. For quality control and detection of adulteration, TLC of (1) glucose, (2) fructose and (3) sucrose on silica gel aluminum foil twice with ethyl acetate – pyridine – water – acetic acid 12:6:2:1 up to 80 mm. Detection in visible light after dipping in diphenylamine - aniline reagent (2 mg diphenylamine and 2 mg aniline HCl were dissolved in 80 mL acetone, 15 mL phosphoric acid were added and the solution was diluted to 100 mL with acetone) and drying at 110 °C for 15 min. The hRf value of (1) was 46, (2) 52 and (3) 39. Linearity was between 6-16 µg/zone for (1)-(3). Repeatability and intermediate precision (%RSD, n=6) for (1), (2), and (3) was 1.2, 1.3, and 1.1 % and 0.1, 1.3, and 1.1 %, respectively. Recovery (by standard addition) was between 100.7 and 102.1 % for all three substances.

      Classification: 10
      112 060
      Determination of biotin in Antarctic krill (Euphausia superba) by high-performance TLC with different post-chromatographic derivatizations
      P. TEO (Teo Peishan), D. LIU (Liu Daicheng)* (*Key Laboratory of Animal Resistance, College of Life Science, Shandong Normal University, 88 East Wenhua Road, Jinan 250014, P. R. China, liudch@sdnu.edu.cn)

      J. Sep. Sci. 36, 2703-2708 (2013). HPTLC of biotin in Euphausia superba on silica gel with dichloromethane - 2-propanol - methanol 3:3:2 + 1 drop glacial acetic acid. Detection (1) by spraying with 0.1 - 1 % 4-(dimethylamino)cinnamaldehyde and sulfuric acid in ethanol, followed by drying for 5 min and spraying with liquid paraffin - chloroform 1:10. Alternatively, biotin was detected (2) by spraying with 0.05 % potassium permanganate. Quantitative determination by absorbance measurement at 530 nm for (1) and 400 nm for (2). The hRf value of biotin was 50. Linearity was in the range of 340-3310 ng/zone for both (1) and (2). LOD and LOQ were 50 and 90 ng/zone for (1) and 60 and 100 ng/zone for (2). Average recoveries were 99.6 % for (1) and 99.7 % for (2). Intermediate intra- and inter-day precision was below 2 % (n=3).

      Classification: 27
      113 061
      High-performance thin-layer chromatographic detection of profenofos in biological materials
      V. CHAVAN, B. MALI* (*Regional Forensic Science Laboratory, State of Maharashtra, Cantonment, Aurangabad 431 002, India, malibdm@yahoo.co.in)

      J. Planar Chromatogr. 27, 66-68 (2014). TLC of profenofos in visceral tissues on silica gel with n-hexane - acetone 4:1. Detection by spraying with 10 % sodium hydroxide solution and after 5-10 min, spraying with diazotized sulfanilic acid reagent (0.5 g sulfanilic acid and 1 g sodium nitrite in 100 mL of 10 % v/v hydrochloric acid). The hRF value for profenofos was 45.

      Classification: 29b
      115 030
      Validated HPTLC fingerprinting and antioxidant activity evaluation of twenty-seven Romanian red wines
      A. HOSU, V. DANCIU, C. CIMPOIU* (*Babes-Bolyai University, Faculty of Chemistry and Chemical Engineering, 11 Arany Janos, 400082 Cluj-Napoca, Romania, ccimpoiu@chem.ubbcluj.ro)

      J. Food Comp. Anal. 41, 174-180 (2015). HPTLC fingerprint of red wines on silica gel with ethyl acetate - formic acid - acetic acid - water 10:1:1:2. Detection by dipping into NP reagent (1 g of diphenylborinic acid aminoethylester was dissolved in 200 mL ethyl acetate), followed by drying in cold air and dipping in PEG reagent (10 g of polyethylene glycol 400 is dissolved in 200 mL dichloromethane). Qualitative identification under UV 254 and 366 nm. Precisions of hRF values (% RSD) of three selected zones on two plates was below 0.02 %.

      Classification: 8a