Biomed. Chromatogr. 21 (10), 1064-1068 (2007). Indirect chiral separation of penicillamine (3,3-dimethylcysteine) enantiomers after derivatization with Marfey's reagent (FDNP-Ala-NH2) and two of its structural variants, FDNP-Phe-NH2 and FDNP-Val-NH2, with phenol - water 3:1 and solvent combinations of acetonitrile and triethylamine phosphate buffer in normal and reversed-phase TLC, respectively. Also separation of the diastereomers on a reversed-phase HPLC column with gradient elution of acetonitrile and 0.01 m trifluoroacetic acid. Comparison of the results due to these three reagents. Successful application of the method for checking the enantiomeric impurity of l-penicillamine in d-penicillamine and to check the enantiomeric purity of pharmaceutical formulations of d-penicillamine.

CBS 97, 9-11 (2006). HPTLC of trigonelline in fenugreek (Trigonella foenum-graecum) on silica gel in a saturated twin-trough chamber with n-propanol - methanol - water 4:1:4 over 80 mm. Quantitative determination by absorbance measurement at 269 nm. The hRf value of trigonelline was 46 and selectivity regarding matrix was given. Linearity was between 100 and 1200 ng/zone. The inter- and intraday precision was below 1 %. The limit of detection and quantification was 2.3 and 7.6 ng/zone, respectively. Recovery (by standard addition) was 99 - 101 %.

59th Indian Pharmaceutical Congress C-220, 277, (2007). HPTLC of gallic acid in fruits of Emblica officinalis, on silica gel with touene - ethyl acetate - acetic acid - formic acid 4:9:4:3. Densitometry at 277 nm for quantitative evaluation. The method was linear in the range of 10 and 1000 ng/mL, with an average recovery of 101.4 %. The method was found suitable for routine quality check-up of Emblica officinalis.

59th Indian Pharmaceutical Congress C-66, 240, (2007). Evaluation of Arjunakhseerpak and Arjunaristha, two ayurvedic formulations used for cardiovascular system, which contain Terminalia arjuna as the main ingredient. Simultaneous HPTLC of arjungenin and arjunolic acid from Terminalia arjuna on silica gel with toluene - ethyl acetate - acetic acid 10:10:1. Detection by spraying with 10 % sulphuric acid followed by heating. Densitometric evaluation at 366 nm. The UV spectra of the sandards arjungenin and arjunolic acid was found to be similar to that of arjungenin and arjunolic acid in the formulation. The method was found suitable for standardization of arjuna formulations.

J. AOAC Int. 90, 1573-1578 (2007). TLC of olanzapine [2-methyl-4-(4-methyl-1-piperazinyl)-10H-thieno[2,3-b] [1,5]benzodiazepine] and fluoxetine [(+/-)-N-methyl-3-phenyl-3-[(alpha,alpha,alpha-trifluoro-p-tolyl)oxy]propylamine] on silica gel using methanol - toluene 2:1 in a twin-trough chamber with chamber saturation. Quantitation by densitometry in absorption mode at 233 nm.

J. Planar Chromatogr. 21, 177-181 (2008). TLC of azithromycin on silica gel with chloroform - ethanol - ammonia 30:70:1. Detection by spraying with sulfuric acid - ethanol 1:4 followed by heating at 120 °C for 5 min. Quantitation by densitometry at 483 nm.

J. Chromatogr. Sci. 46 (1), 10-14 (2008). Quantitative determination of rabeprazole and mosapride in their combined pharmaceutical dosage forms by TLC on silica gel with ethyl acetate - methanol - benzene 4:1:5. Quantification by densitometry at 276 nm. Linearity was between 400 and 1200 ng/spot for rabeprazole and between 300 and 900 ng/spot for mosaprid, respectively. The limit of detection was 132 ng/spot for rabeprazole and 98 ng/spot for mosapride.

Isolation of the enantiomers. Chromatographia 68 (11-12), 1048-1051 (2008). TLC resolution of the enantiomers of the racemic drugs ketamine and lisinopril on silica gel with 1) ethyl acetate - methanol - water 3:1:1 and enantiomerically pure tartaric acid and (-)-mandelic acid as chiral impregnating reagents, for ketamine; and 2) acetonitrile - methanol - water - dichloromethane 14:2:2:1 with (+)-tartaric acid as the impregnating agent for lisinopril and using (+)-tartaric acid as mobile phase additive; and 3) acetonitrile - methanol - (+)-tartaric acid (0.5 % in water, pH 5) - acetic acid 70:10:11:7 which enabled successful resolution of the enantiomers of lisinopril. Investigation of the effects of temperature, pH, and the amount of chiral selector. Detection with iodine vapour. Isolation and identification of the separated enantiomers. The LOD was 0.25 and 0.27 µg for each enantiomer of ketamine with (+)-tartaric acid and (-)-mandelic acid, respectively, whereas 0.14 and 0.16 µg for each enantiomer of lisinopril with (+)-tartaric acid (both conditions) and (-)-mandelic acid, respectively.