J. Pharm. Biomed. Anal. 38, 180-185 (2005). A stability indicating HPTLC method is reported for estimation of cyclophosphamide in pharmaceutical preparations. HPTLC on silica gel with dichloromethane - methanol - acetic acid 97:3:2. Detection with alcoholic phosphomolybdic acid solution followed by heating at 190 °C for 10 min. Quantitative determination by absorbance measurement at 700 nm. Linearity was obtained between 0.40-1.20 mg/mL with recovery rates of 99.5 %. The method was validated for selectivity, specificity, accuracy, precision, and found to be stable for 28-70 days.

J. Planar Chromatogr. 18, 282-284 (2005). TLC and HPTLC of horseradish and mustard samples on silica gel with iso-propanol - 25 % ammonia 9:1 containing different volumes of water (1, 2, 3, or 5 parts). After development the compounds were visualized under UV light at 254 nm or by exposure to iodine vapor.

J. AOAC Int. 88, 1571-1577 (2005). TLC and HPTLC of narceine, morphine, codeine, thebaine, papaverine, and narcotine on silica gel with toluene - acetone - ethanol - 25% ammonia 20:20:3:1. Detection with Dragendorff’s reagent with sodium nitrite, densitometric evaluation at 520 nm. For UV-detection, Naturstoff reagent A (diphenylboric acid 2-amino ethyl ester) was used; quantitation by densitometry at 310 nm.

J. Chinese Trad. Patent Med. (Zhongchengyao) 27 (7), 786-790 (2005). TLC of Hainan holly on silica gel with 1) ethyl acetate - butanone - formic acid - water 5:3:1:1; 2) ethyl acetate - formic acid - water 14:5:5; 3) benzene - acetone - methanol - formic acid 85:15:10:2. Detection 1) by spraying with 1 % AlCl3 solution; 2) under UV 365 nm; 3) by spraying with 5 % phosphomolybdic acid in ethanol followed by heating at 105 ºC until the spots are visualized. Identification by fingerprint techniques. Quantification of rutin by HPLC. Discussion of the optimum processing procedure by comparison of TLC fingerprints and HPLC results of rutin content.

J. Chinese Trad. Patent Med. (Zhongchengyao) 27 (7), 859-861 (2005). TLC of Ganlu Xiaodu capsules on silica gel with chloroform - methanol - water 185:15:2. Detection under UV 254 nm. Identification by fingerprint techniques and by HPLC. Quantification of scutellarin by HPLC.

et Zucc by thin-layer chromatography) (Chinese). J. Chinese Trad. Patent Med. (Zhongchengyao) 27 (5), 605-607 (2005). TLC of resveratrol on silica gel with petroleum ether (30 ºC-90 ºC) - ethyl acetate - methanol - glacial acetic acid 200:50:35:1. Detection under UV light. Identification by comparison with the standard. Quantification by densitometry at 293 nm. Validation of the method by investigation of its linearity range (0.5 µg - 5.0 µg, r = 0.999); precision (RSD = 0.98 %, n = 5); its reproducibility of five time assay towards the same sample (RSD = 2.52 %); standard addition recovery (101.6 %, RSD = 0.26 %, n = 5). The results for three real life samples are given. Discussion of the application of the procedures for the quality control of the medicine.

by thin-layer chromatography) (Chinese). J. Chinese Trad. Patent Med. (Zhongchengyao) 27 (8), 941-944 (2005). TLC of betaine on silica gel with acetone - ethanol - hydrochloric acid 10:6:1. Detection by spraying with a solution of potassium iodobismuthate. Quantitative determination by densitometry at 515 nm. Validation regarding linearity range (8.0 - 39.0 µg, r = 0.9992), precision (RSD = 1.62 %, n = 6), reproducibility (RSD = 2.14 %, n = 6), and recovery (99.75 %, RSD = 1.92 %, n = 6). Results are given for 12 real life samples. Discussion of the advantages of the method compared to HPLC.

J. AOAC Int. 89, 995-998 (2006). HPTLC of phenobarbital on silica gel, prewashed with methanol and then acetone, using dichloromethane - ethyl acetate - formic acid 95:5:1 in a horizontal chamber. Quantitation by densitometry in the absorbance/reflectance mode at 210 nm. The validity of the HPTLC-densitometric method was established through a study of linearity, sensitivity, accuracy, and reproducibility.