J. of Chinese Med. 26 (169), 716-718 (2012). Shengma Gegen granules are a herbal TCM preparation for the treatment of viral hepatitis type C, drug-induced liver diseases, and postherpetic neuralgia. For quality control, TLC on silica gel 1) for Rhizoma Cimicifugae, with benzene – chloroform – glacial acetic acid 12:2:1, detection under UV 366 nm; 2) for Radix Paeoniae Rubra, with chloroform – ethyl acetate – methanol – formic acid 200:25:50:1, detection by spraying with 2 % vanillin in sulfuric acid – ethanol 1:200 and heating mildly until the zones are visible in daylight. Quantification of puerarin by HPLC.

Chinese J. of Med. Guide 10 (17), 98-99 (2012). Banlian Chongji electuary is herbal TCM preparation for the treatment of virus flu and upper respiratory infections. For quality control the indirubin and indigo contents are determined by TLC on silica gel with petroleum ether (60-90 °C) – ethyl acetate – chloroform 1:1:8. Detection in daylight, quantification by densitometry at 290 nm. The linearity was in the range of 0.1-0.5 µg/zone (n=5, r=0.9999) both for indirubin and indigo. The precision (%RSD, n=5) was 1.2 % for indirubin and 2.2 % for indigo. Recovery by standard addition was 95.5 % (%RSD=4.5 %, n=5) for indirubin, and 96.0 % (RSD%=2.9 %, n=5) for indigo.

Jiangxi J. of Trad. Chinese Med. 44 (367), 58-60 (2013). Zhongfeng Fuyuan Koufuye oral liquid is a herbal TCM preparation for the treatment of post stroke shoulder hand syndrome during stroke recovery and sequela period, and constipation after stroke. For quality control, TLC on silica gel 1) for Astragalus membranaceus (Fisch.) Bunge., with n-butanol – ethyl acetate – 10 % diluted ammonia 4:1:5, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C and viewing in daylight; 2) for Pericarpium Citri Reticulatae, with toluene – ethyl acetate 3:2, detection by heating at 105 °C for 3 min and viewing under UV 366 nm; 3) for Paeonia veitchii, with toluene – ethyl acetate – isopropanol – methanol – ammonia 20:10:5:5:1, detection by exposure to ammonia vapors for 15 min and spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C, viewing in daylight. Quantification of astragaloside A by HPLC.

J. Planar Chromatogr. 27, 174-180 (2014). HPTLC of betulinic acid (1), 24beta-ethylcholesta-5,22E,25-triene-3beta-ol (2) and lupeol (3) in Clerodendrum phlomidis on silica gel with chloroform - methanol 49:1. Detection by dipping into vanillin - sulfuric acid reagent (vanillin - ethanol - sulfuric acid 1:95:5, w/V/V), followed by heating at 110 ºC for 3 min. Quantitative determination by absorbance measurement at 600 nm. The hRF values for (1) to (3) were 30, 52 and 70. Linearity was in the range of 300-1500 ng/mL. The intermediate/interday/intra-day precisions were below 2 % (n=5). The LOD and LOQ were 22 and 74 ng/zone for (1), 20 and 66 ng/zone for (2) and 30 and 100 ng/zone for (3), respectively. Average recovery was 99.0 % for (1), 98.5 % for (2) and 98.2 % for (3).

J. Planar Chromatogr. 27, 120-123 (2014). HPTLC of azathioprine on silica gel with methanol - toluene - 25 % ammonia 70:30:1. Quantitative determination by absorbance measurement at 285 nm. The hRf value for azathioprine was 63. Linearity was in the range of 20-120 ng/zone. The intermediate/interday/intra-day precisions were below 1 % (n=6). The LOD and LOQ were 6 and 20 ng/zone, respectively. Average recovery was 98.7 %.

J. Planar Chromatogr. 27, 217-229 (2014). HPTLC of omeprazole (1), panoprazole (2), rabeprazole (3), and lansoprazole (4) in binary mixtures with domperidone (5) (method A) and ternary mixture containing omeprazole (1), tinidazole (6), and clarithromycin (7) (method B) on silica gel with ethyl acetate - methanol - benzene - acetonitrile 5:4:8:3 for (A) and chloroform - n-hexane - methanol 3:4:1 for (B). Quantitative determination by absorbance measurement at 293 nm for (1)/(5) and (3)/(5), 286 nm for (4)/(5), 287 nm for (2)/(5), and 310 nm for (1)/(6)/(7). The hRF values for (1) to (5) in (A) were 54, 55, 55, 60 and 33, respectively, and for (1), (6) and (7) in (B) were 68, 22 and 48, respectively. Linearities for (1) to (7) were in the range of 240-1440 ng/zone, 180-1080 ng/zone, 240-1440 ng/zone, 240-1440 ng/zone, 120-720 ng/zone, 30-180 ng/zone and 2250-6000 ng/zone, respectively. The intermediate/interday/intra-day precisions were below 4 % (n=6). Recoveries ranged from 98.1 to 103.1 % for (1) to (7).

J. Planar Chromatogr. 27, 2942-2955 (2014). HPTLC of propafenone hydrochloride in tablets on silica gel with chloroform - methanol - acetic acid 79:20:1. Quantitative determination by absorbance measurement at 316 nm. The hRF value for propafenone hydrochloride was 64. Linearity was in the range of 100-3200 ng/zone. The intermediate/interday/intra-day precisions were below 2 % (n=3). The LODs and LOQs were 20 and 80 ng/zone, respectively. Recoveries were between 99.5 and 102.2 %.

Chinese J. of Forestry Science 49 (10), 127-134 (2013). Flavonoids in bamboo leaves have free radical scavenging, antioxidant, anti-aging, antibacterial, and anti-inflammatory effects and are used as a component in TCM for regulating blood fat and preventing cardiovascular and cerebrovascular diseases. Bamboo-leaf flavonoids are also used in cosmetics and as feed additive. In order to choose the best bamboo species for extraction of flavonoids and to set up a quality control method, the flavonoids in eleven samples of bamboo leaves of three genera collected from Yunnan, Fujian, Sichuan, Jiangsu and Jiangxi provinces are studied by HPTLC. HPTLC-AMD of sample extracts and the standards isoorientin, orientin, isovitexin, vitexin and tricin on silica gel, cleaned with methanol and methylene chloride and dried at 105 °C, with methanol – ethyl acetate – methylene – formic acid 4:7:9:2 to 50 mm in the first step, with acetone – methanol - ethyl acetate – methylene chloride – formic acid 1:2:7:10:2 to 75 mm in the second step, and with acetone - methanol - ethyl acetate - methylene chloride – formic acid 2:1:6:11:2 to 90 mm in the third step. Detection under UV 366 nm after spraying with 1 % aluminum trichloride in ethanol. Quantification of the flavonoids by densitometry at UV 366 nm via peak area. The quantitative method for different flavonoids was validated by investigation of the linearity (90-1750 ng/zone), the precision (%RSD=1.0-2.0 %, n=3 intra-day; %RSD=0.9-2.0 %, n=3 inter-day), and the repeatability (%RSD=0.9-1.9 %, n=9). The LODs were 25-40 ng/zone, and the recoveries were 81.3-106.9 % (n=3).