Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

  • Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
  • Browse and search by CBS classification: Select one of the 38 CBS classification categories where you want to search by a keyword
  • Keyword register: select an initial character and browse associated keywords
  • Search by CBS edition: Select a CBS edition and find all related publications

Registered users can create a tailor made PDF of selected articles throughout CCBS search – simply use the cart icon on the right hand of each abstract to create your individual selection of abstracts. You can export your saved items to PDF by clicking the download icon.

Page
      130 004
      Identification of acetylcholinesterase inhibitors in water by combining two-dimensional thin-layer chromatography and high-resolution mass spectrometry
      Lena STÜTZ*, W. SCHULZ, R. WINZENBACHER (*Laboratory for Operation Control and Research, Zweckverband Landeswasserversorgung, Langenau, Germany; stuetz.l@lw-online.de)

      J Chromatogr A, 1624, 461239 (2020). Samples were chemical standards of acetylcholinesterase (AChE) inhibitors (azamethiphos, caffeine, donepezil, galanthamine, methiocarb-sulfoxide, paraoxon-ethyl) and of neurotoxic compounds, as well as drinking or contaminated water samples enriched through solid phase extraction. HPTLC on spherical silica gel (pre-washed twice by 20 min immersion in isopropanol, heated 20 min at 120 °C before and after pre-washing with acetonitrile). First separation (preparative TLC) with automated multiple development (16 steps). Effect-directed analysis for AChE inhibitors by immersion (speed 5 cm/s, time 1 s) into enzyme solution, incubation 5 min at 37 °C and immersion into substrate solution (indoxyl acetate 2 % in methanol); visualization under UV 366 nm. Active zones from untreated layers were eluted through the oval head of a TLC-MS interface to a second plate for a second separation with a panel of other mobile phases. Bands of interest were eluted from the second layer with water through the oval elution head of the TLC-MS interface pump, into a RP18 liquid chromatography guard column, followed by a quadrupole time-of-flight mass spectrometer. Full scan mass spectra (m/z 100–1200) were recorded in negative and positive modes using electrospray ionization (and collision-induced dissociation for MS2). Among the water contaminants, lumichrome (riboflavin photolysis product), paraxanthine and linear alkylbenzene sulfonates were identified as AChE inhibitors.

      Classification: 3d, 4d, 4e, 22, 29b, 35d, 37c
      130 005
      Multiobjective optimization of microemulsion – thin layer chromatography with image processing as analytical platform for determination of drugs in plasma using desirability functions
      Noura H. ABOU-TALEB*, D. T. EL-SHERBINY, N. M. EL-ENANY, H. I. EL-SUBBAGH (*Medicinal Chemistry Department, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt; nourahemdan@yahoo.com)

      J Chromatogr A, 1619, 460945 (2020). Samples were lamotrigin as standard, or extracted with an oil-in-water microemulsion (10 µL butyl acetate, 4 mL n-butanol, 925 mg sodium dodecyl sulphate, 8.6 mL water) either from patients’ raw plasma (for separation from blood proteins) after spiking, or from commercial tablets dissolved in methanol. TLC on silica gel with a water-in-oil microemulsion of 9 mL butyl acetate, 1 mL n-butanol, 250 mg sodium dodecyl sulphate, 250 µL water. Both optimal microemulsions were predicted using Taguchi orthogonal array and Plackett-Burman design. Evaluation in UV 254 nm, quantification from the digital picture using four image processing software programs. For lamotrigin (hRF 24), limits of quantification were 170 ng for pure drug and 10 ng for spiked plasma. Linearity (in range 20–200 ng/spot) was directly obtained for the calibration curve in spiked plasma; however, for pure drug, linearity was obtained only when using log values of the calculated densities (300–3000 ng/spot).

      Classification: 3a, 3d, 5c, 23e, 32c
      130 113
      Recent findings by high‑performance thin‑layer chromatographic separation for a comprehensive analysis of Withania somnifera by densitometry and mass spectrometry: an assessment to quality and adulteration
      S. GHOSHAL, C. GHULE, A. MIRGAL, A. GIRME*, L. HINGORANI (*Pharmanza Herbal Pvt. Ltd., Anand, Gujarat 388430, India, ardm@pharmanzaherbals.com)

      J. Planar Chromatogr. 35, 439-451 (2022). HPTLC of withanoside IV (1), withanoside V (2), withaferin A (3), and kaempferol-based glucoside (4) in the roots and aerial parts of Withania somnifera on silica gel with ethyl acetate - chloroform - methanol - water 40:15:22:9. Detection of (1) to (3) by spraying with anisaldehyde sulfuric acid reagent, followed by heating at 100 °C for 3 min. Quantitative determination by absorbance measurement at 540 nm for (1) to (3) and 254 nm for (4). The hRF values for (1) to (4) were 33, 42, 62 and 21, respectively. Linearity was between 200 and 1000 ng/zone for (3) and (4) and 400 and 2000 ng/zone for (1) and (2). Interday and intra-day precisions were below 4 % (n=6). The LOD and LOQ were 180 and 544 ng/zone for (1), 215 and 652 ng/zone for (2), 170 and 516 ng/zone for (3) and 48 and 144 ng/zone for (4). Recovery was between 95.9 and 99.6 % for (1) and (4).

      Classification: 8a, 14
      130 114
      Simultaneous ultra‑sensitive analysis of tamsulosin hydrochloride and tolterodine tartrate binary mixture in their dosage form via high‑performance thin‑layer chromatography with fluorimetric detection
      M. RIZK, Z. MAHMOUD*, M. AZAB (*Analytical Chemistry Department, Faculty of Pharmacy, Helwan University, Cairo 11795, Egypt, Zainab.MansourMahmoud@pharm.helwan.edu.eg)

      J. Planar Chromatogr. 35, 509-517 (2022). HPTLC of tamsulosin hydrochloride (1) and tolterodine tartrate (2) binary mixture on silica gel with ethyl acetate - n-hexane - diethylamine 9:3:1. Quantitative determination by absorbance measurement at 225 nm. The hRF values for (1) and (2) were 40 and 85, respectively. Linearity was between 10 and 200 ng/zone for (1) and 100 and 900 ng/zone for (2). The LOD and LOQ were 3 and 8 ng/zone for (1) and 22 and 66 ng/zone for (2), respectively. Average recovery was 100.1 % for (1) and 100.7 5 for (2).

      Classification: 32a
      130 115
      ICH and US‑FDA validated HPTLC methods with greenness assessments for the assay of mixtures prescribed in stroke prophylaxis: application to pharmaceutical preparations and human plasma
      M. HAMDY*, M. KORANY, S. EBIED, R. HAGGAG (*Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Pharos University in Alexandria, Alexandria, Egypt, mohamed.hamdy@pua.edu.eg)

      J. Planar Chromatogr. 35, 519-532 (2022). HPTLC of binary mixtures of the novel oral anticoagulants (NOACs) apixaban (1), edoxaban tosylate (2) and rivaroxaban (3) with the lipid-lowering statin, rosuvastatin calcium (4) on silica gel with toluene - ethyl acetate - methanol - 25 % ammonia 35:45:20:2 (method 1) for the three mixtures, and methanol - 25 % ammonia 199:1 (method 2) for (2)/(3) mixture only. Quantitative determination by absorbance measurement at 291 nm. The hRF values for (1) to (4) were 65, 20, 75 and 10 using method 1, and 40 for (2) and 90 for (4) using method 2. Linearity was between 5 and 45 µg/mL for (1) to (4). Interday and intra-day precisions were below 3 % (n=6). The LOD and LOQ were 0.1 and 0.4 µg/mL for (1), 1 and 4 µg/mL for (2) and (3), 0.4 and 1 µg/mL for (4) using method 1, and 1.4 and 4.7 µg/mL for (2) and 0.4 and 1.2 µg/mL for (4) using method 2. Average recovery was between 97.6 and 102.9 % for (1) to (4).

      Classification: 32a
      130 117
      Combination of high‑performance thin‑layer chromatography and liquid chromatography–quadrupole time‑of‑flight–tandem mass spectrometry analysis: a promising analytical tool for discrimination between oleo‑gum resin of raw and purified Commiphora wightii
      V. CHARDE, C. JAGTAP, Y. GANDHI, R. VERMA, S. MISHRA, V. KUMAR*, R. ACHARYA (*Department of Ayurveda, Central Ayurveda Research Institute, Jhansi, Uttar Pradesh 284003, India, vijaychem99@gmail.com)

      J. Planar Chromatogr. 35, 481-490 (2022). HPTLC of oleo‑gum resin of raw and purified Commiphora wightii on silica gel with chloroform - ethyl acetate - formic acid - acetic acid
      30:9:2:2. Detection by spraying with anisaldehyde sulfuric acid reagent, followed by heating at 105 °C until the development of visible zones. The plate was analyzed under UV light at 254 and 366 nm, and after derivatization at 541 nm. 

      Classification: 32e
      130 118
      A validated method for the thin‑layer chromatography in situ densitometric quantitation of capsaicinoids in Habanero pepper (Capsicum chinense Jacq.)
      A. CORDOVA, M. MONFORTE, A. ROZETE, N. ESTRADA, F. VAZQUEZ* (*Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, 97205 Mérida, Yucatán, Mexico, felipe@cicy.mx)

      J. Planar Chromatogr. 35, 473-479 (2022). HPTLC of capsaicin and dihydrocapsaicin as capsaicinoids in Habanero pepper pods on silica gel with cyclohexane - chloroform - acetic acid 7:2:1. Quantitative determination by absorbance measurement at 254 nm. The hRF value for capsaicinoids was 29. Linearity was between 0.5 and 4.0 µg/zone. Interday and intra-day precisions were below 6 % (n=6). The LOD and LOQ were 251 and 750 ng/zone, respectively. 

      Classification: 17c
      130 119
      Development of an identification method for fern extracts using high‑performance thin‑layer chromatography (HPTLC)
      Melania ENOT*, R. SABESAJE, G. PRESORES, G. BARBOSA, A. ANG, R. BAUTISTA, R. DE LA CRUZ (*Tuklas Lunas Development Center, Central Mindanao University, University Town, Musuan, 8714 Bukidnon, Philippines, melaniaenot@cmu.edu.ph)

      J. Planar Chromatogr. 35, 491-500 (2022). HPTLC of gallic acid in fern species Drynaria
      quercifolia, Diplazium esculentum, and Asplenium nidus
       on silica gel with ethyl acetate - formic acid - acetic acid - water 120:11:11:26. Detection by heating at 100 °C for 3 min, followed by dipping into NP reagent (1.0 g of 2-aminoethyl diphenyl borate in 200 mL of ethyl acetate and stored at 4 °C).
      Quantitative determination by absorbance measurement at 366 nm. The hRF value for gallic acid was 82. Interday and intra-day precisions were below 1 % (n=3). 

       

      Classification: 7
Page