Chinese J. Trad. Pat. Med. (Zhongchengyao) 26 (9), app.17-19 (2004). HPTLC on silica gel with 1) toluene - acetone - ethanol - ammonia 20:25:3:2; 2) n-hexane - ethyl acetate - glacial acetic acid 15:5:1; 3) n-hexane - ethyl acetate - ammonia 20:20:1. Detection 1) by spraying with 5 % potassium iodobismuthate solution; 2) by spraying with 1 % potassium permanganate in diluted sulfuric acid followed by heating at 120 ºC, and under UV 360 nm. Identification by fingerprint technique. Determination of the content of aconitine by comparison with standard.

J. Planar Chromatogr. 17, 238-240 (2004). HPTLC of aceclofenac and mosapride citrate (as internal standard) on silica gel in a twin-trough chamber equilibrated with the mobile phase with toluene - methanol - ethyl acetate - glacial acetic acid 550:250:200:1. Quantitative determination by densitometry at 284 nm.

J. Planar Chromatogr. 18, 221-223 (2005). HPTLC of horminone on silica gel in a twin-trough chamber with hexane - dioxane 9:1. Absorbance measurement at 271 nm. For fluorescence analysis the plates were dipped in 1 % diphenylboryloxyethylamine in ethyl acetete for 2 s, followed by drying and dipping in a solution of 5 % PEG 8000 in dichloromethane for 2 s. After 15 min fluorescent zones of horminone were scanned at 366/>400 nm. Use of the fluorescence reagent successfully reduced the limits of detection and quantification to 0.75 ng/spot and 1.51 ng/spot, respectively. Linearity range was from 60-300 ng/spot.

J. Chinese Trad. Patent Med. (Zhongchengyao) 27 (4), 410-414 (2005). TLC of the extracts of the title Chinese traditional patent medicine on silica gel with 1) chloroform - ethyl acetate - methanol - formic acid 400:25:50:1 2) the lower phase of chloroform - methanol - water 32:17:5; 3) n-hexane - chloroform - water 15:8:2. Detection 1) by spraying with 5 % vanillin - H2SO4 solution and heating mildly until the spots are visualized; 2) by spraying with 2 % AlCl3 in methanol and inspection under UV 365 nm; 3) by exposing to iodine vapour. Identification by fingerprint techniques. Quantification of paeoniflorin by HPLC.

J. AOAC Int. 88, 1530-1535 (2005). HPTLC of ciprofloxacin and degradation products (an ethylenediamine compound, a desfluoro compound, a by-compound, and fluoroquinolonic acid) on silica gel with chloroform - methanol - 25 % ammonia 43:43:14. Quantitative determination by densitometric analysis at 330 nm for fluoroquinolonic acid and at 277 nm for the other compounds. The method showed high sensitivity (limit of detection 10 to 44 ng), a wide linearity range (3 to 20 µg/mL), and good precision (2.32 to 6.46 % relative standard deviation) and accuracy (recovery rates 98.6 to 101.5 %) for individual constituents.

J. Chinese Trad. Patent Med. (Zhongchengyao) 27(5), 535-538 (2005). TLC of the extracts on silica gel with 1) benzene - ethyl acetate - formic acid 15:2:1; 2) n-hexane - ethyl acetate - formic acid 60:20:1; 3) chloroform - methanol - ammonia 40:10:1; 4) chloroform - methanol - water 13:7:2. Detection 1) under UV 365 nm; 2) by spraying with 3 % ninhydrin solution followed by heating at 105 ºC until the spots are visualized; 3) by spraying with 10 % H2SO4 solution in ethanol followed by heating until the spots are visualized. Identification by fingerprint technique. Quantification of emodin by densitometry at 445 nm. Validation of the method by investigation of its linearity range (0.1 µg - 1.0 µg, r = 0.998); precision (RSD = 1.05 % n = 6); reproducibility of six time assay towards the same sample (RSD = 1.24 %); and standard addition recovery (96.7 %, RSD = 1.75 %, n = 6). The results for three batches of real life sample are given.

J. Pharm. Biomed. Anal 36, 617-620 (2004) HPTLC of caffeine in herbal products and energy drinks, on silica gel with ethyl acetate - methanol 17:3. Solid samples (capsules) were extracted with methanol, filtered and applied whereas liquid samples (coca cola) were applied after the effervescence has ceased. Quantitative determination by absorbance measurement at 275 nm. The developed method was validated for specificity, repeatability (CV < 5 %), recovery (98.90) and accurary (99.84). The levels of caffeine were 4.76-13.29 % and 0.011-0.032 % for the herbal products and the energy drinks resp.

J. Planar Chromatogr. 19, 223-227 (2006). HPTLC of leuprolide acetate (a synthetic nonapeptide analog) on silica gel after prewashing with chloroform - methanol 1:1 using five-step isocratic incremental multiple development with ethyl acetate - methanol - 25 % ammonia. Detection under UV light at 254 and 280 nm. Quantitation by reflectance scanning at 280 nm.