& Wendl. (Solanaceae) against ethylene glycol induced urolithiasis in rats. J. Ethnopharmacol. 138, 160-170 (2012). HPTLC of solasodine in the fruits of Solanum xanthocarpum on silica gel with toluene - ethyl acetate - diethylamine 12:1:1. Quantitative determination by absorbance measurement at 200 nm. The hRf of solasodine was 52.

J. Planar Chromatogr. 25, 283-289 (2012). HPTLC of mesembranol (1), mesembrenol (2), mesembrine (3) and mesembrenone (4) in the aerial parts of Sceletium tortuosum on silica gel with dichloromethane - methanol 9:1 + 1 drop ammonia. Quantitative determination by absorbance measurement at 280 nm. The hRf values for compounds (1) to (4) were 8, 28, 60 and 71, respectively. Linearity was in the range of 180-240 ng/band for (1) to (3) and 60-300 ng/band for (4). Limits of detection and quantification were 25 and 75 ng/band for (1), 31 and 95 ng/band for (2), 27 and 80 ng/band for (3) and 18 and 44 ng/band for (4), respectively. The intermediate/inter-day/intra-day precision was below 1.6 %. Mean recovery for the compounds was between 90.1 and 104.7 %.

by thin-layer chromatography) (Chinese). J. of Guizhou Normal Univ. (Natural Sci.) 30 (3), 7-8 (2012). Saxifraga stolonifera (L.) Meerb is a TCM herb for heat-clearing and detoxification and is often used as the key component in the preparations for curing otitis media, traumatic bleeding and hyperplasia of the prostate. TLC of the extracts of the whole medicinal herb and gallic acid as standard on polyamide phase with chloroform – methanol – formic acid 25:5:1, detection by spraying with a solution of ferric chloride – 2N hydrochloric acid – water 2:1:100 and heating until the zones are visible, viewing in daylight.

J. of China Pharm. 21 (4), 30-31 (2012). Shenqixinjikang compound oral liquid, as a TCM preparation is specially effective for invigorating the circulation of blood, inducing resuscitation, relieving pain, and is prescribed clinically to cure cardiopalmus and angina pectoris. For quality control, TLC of the extracts of the medicine 1) for Rhizoma Polygoni Cuspidati, on silica gel with petroleum ether ( 60-90 ºC) – formyl acetate – formic acid 15:5:1, detection at UV 366 nm; 2) for Leguminosae, on silica gel with n-butanol – acetic acid – water 5:1:4, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C for 5 min followed by evaluation under daylight or UV 366 nm; 3) for Rehmannia, on silica gel with chloroform – methanol – water 14:6:1, detection by spraying with 3 % vanillin in ethanol – sulfuric acid 100:1 and heating at 105 °C followed by evaluation under daylight.

Chinese J. of Lishizhen Trad. Med. & Pharm. 22(1), 133-134 (2011). Capparis spinosa L. is a plant with an important ecological function which is mainly growed in the sand of the Gobi desert. It has a huge root system which helps to maintain the humidity of the land. As a Uygur traditional medicinal herb, it is effective in curing diseases like rheumatism, toothache, and dysentery. However, hitherto only Capparis spinosa root has been used in the preparations. This wrecks seriously the resource of the herbal drug and its ecological function. Therefore, its fruits have recently been applied as replacement for the root in the preparations. Quality control of the fruits by TLC on silica gel with chloroform – methanol – formic acid – water 20:90:10:3. Detection by spraying with 5 % potassium iodobismuthate solution. The fingerprints were compared with the extracts from the root, taking one of the typical components, betaine, as the marker substance. In addition the fruits were identified based on their shape, size, surface colors, smell, and the sarcocarp; by microscopy and by determination of the moisture content.

Chinese J. of Lishizhen Trad. Med. & Pharm. 23 (2), 401-402 (2012). The dried root of Anemarrhena asphodeloides Bge. is a traditional Chinese medicinal crude drug specially effective for cleaning heat, purging the pathogenic fire, reinforcing body fluid and nourishing the blood, promoting the secretion of saliva or body fluids. Timosaponin B-II is a water soluble saponin extracted from Anemarrhenae. TLC of rhizoma anemarrhenae total steroidal saponins on silica gel with n-butanol – glacial acetic acid – water 4:1:5, detection by spraying with 10 % vanillin in ethanol and heating at 105 °C until the zones are visible, evaluation in daylight.

J. Liq. Chromatogr. Relat. Technol. 36, 700-716 (2013). HPTLC of sertraline in bulk and formulation on silica gel with toulene - ethyl acetate 1:5+1 drop ammonia. Quantitative determination by absorbance measurement at 273 nm. The hRf value of sertraline was 70. Linearity was in the range of 25-2000 ng/zone. LOD and LOQ were 15.3 and 46.8 ng/zone. Intermediate precision was below 0.83 %. Recovery range was in the range of 99.6-100.3 %. The hRf values of acid and base-induced degradation products were 40, 52, 90 and 25.

Jiangxi J. of Trad. Chinese Med. 43 (354), 62-64 (2012). Jiegu Xujin capsules are a herbal TCM for the treatment of fractures, bone dislocation and joint pain. For quality control, TLC on silica gel 1) for Rhizoma Drynariae and the standard naringin, with cyclohexane – ethyl acetate – formic acid – water 2:24:5:6, detection under UV 366 nm; 2) for Radix Angelicae Sinensis and Rhizoma Ligustici Chuanxiong, with n-hexane – ethyl acetate 9:1, detection under UV 366 nm; 3) for Eupolyphaga seu steleophaga, with toluene – dichloromethane – acetone 10:10:1, detection by spraying with 3 % vanillin in sulfuric acid – ethanol 1:200 and heat at 105 °C, viewing in daylight; 4) for Radix Notoginseng and the standards ginsenoside Rb1, Re, Rg1, R1, with the lower phase of chloroform – methanol – water 13:7:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing under UV 366 nm.