ex O.Berg) for its major anthocyanins by densitometry. J. Planar Chromatogr. 26, 363-369 (2013). HPTLC of delphinidin-3,5-diglucoside (1), petunidin-3,5-diglucoside (2), and malvidin-3,5-diglucoside (3) in the fruits of Eugenia jambolana on silica gel with ethyl acetate - formic acid - acetic acid - water - methanol 126:19:10:32:12. Quantification by absorbance measurement at 529 nm. The hRf values for (1), (2) and (3) were 17, 24 and 34, respectively. Linearity was in the range of 400-1000 ng/zone for (1) and (2) and 200-500 ng/zone for (3). LOD and LOQ were 84 and 283 ng/zone for (1), 72 and 242 ng/zone for (2) and 43 and 154 ng/zone for (3), respectively. Average recoveries were 95.4 % for (1), 96.3 % for (2) and 97.3 % for (3). Intermediate/interday/intra-day precision was below 5 % (n=3).

J. Planar Chromatogr. 26, 260-266 (2013). HPTLC of of iridoids 10-O-trans-p-coumaroylcatalpol (1), 4-hydroxy-E-globularinin (2) and premnosidic acid (3) in the stem bark of Premna integrifolia on silica gel with ethyl acetate - methanol - water - acetic acid 40:6:3:1. Detection by dipping into vanillin - sulphuric acid reagent (1 % vanillin in ethanol - sulfuric acid 19:1), followed by heating at 110 ºC for 3 min. Quantification by absorbance measurement at 510 nm. The hRf values for (1) to (3) were 52, 41 and 33, respectively. Linearity was in the range of 1-10 µg/zone for (1) to (3). LOD and LOQ were 198 and 663 ng/zone for (1), 312 and 1040 ng/zone for (2) and 200 and 666 ng/zone for (3), respectively. Average recoveries for (1) to (3) were found to be 97.3 %, 98.3 % and 97.6 %, respectively. Intermediate/interday/intra-day precision was below 2 % (n=9).

Chinese J. of Northern Pharmacy 10 (10), 9-10 (2013). Keke Diwan drop pills are a herbal TCM preparation for the treatment of cough, dyspnea and shortness of breath. For quality control, TLC on silica gel 1) for Ephedra sinica Stapf and the standard ephedrine hydrochloride, with chloroform – methanol – ammonia 100:20:1, detection by spraying with 0.3 % ninhydrin in n-butanol – glacial acetic acid 19:1 and heating at 105 °C, viewing in daylight; 2) for Pericarpium papaveris, with toluene – acetone – ethanol – concentrated ammonia 20:20:3:1, detection under UV 366 nm; 3) for Glycyrrhiza uralensis Fisch., with benzene – chloroform – methanol – glacial acetic acid 40:90:15:1 with preconditioning for 10 min, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight.

J. Planar Chromatogr. 26, 226-231 (2013). HPTLC of lamivudine (1), tenofovir disoproxil fumarate (2), and efavirenz (3) in tablets on silica gel with toluene - methanol 27:6. Quantitative determination by absorbance measurement at 254 nm. The hRf values for compounds (1) to (3) were 12, 16 and 53, respectively. Linearity was in the range of 375-900 ng/zone for (1) and (2) and 750-1800 ng/zone for (3). LOD and LOQ for (3) were 1 and 3 ng/zone. Recovery was in the range of 98.3-102.9 % for (1) to (3). Intermediate/interday/intra-day precision was below 2 % (n=6).

root by thin-layer chromatography) (Chinese). J. of Trad. Chinese Med. & Pharm. Consult. 4 (5), 16-17 (2012). The root of Chinese Eupatorium L. is a herbal TCM drug used in various preparations for the treatment of diphtheria, sore throat, fever, cough, traumatic swelling pain and snake bites. For quality control and to prevent counterfeiting TLC of herbal extracts and the standard euparin on silica gel with n-hexane – chloroform – methanol 20:10:1, detection by spraying with 1 % aluminium chloride in ethanol and heating until the zones are visible in daylight, and under UV 366 nm.

J. Planar Chromatogr. 26, 96-101 (2013). HPTLC of cetylpyridinium chloride (1), sodium dodecyl sulfate (2) and Triton X-100 (3) on silica gel with methanol - water 1:1. Quantitative determination by absorbance measurement at 254 nm. The hRf values for (1) to (3) were 6, 74 and 91, respectively. Linearity was in the range of 170-1740 µg/zone for (1), 250-1980 µg/zone for (2) and 160-1640 µg/zone for (3). LOQ was 75 ng/zone for (1), 90 ng/zone (2) and 65 ng/zone for (3).

Food Addit. Contam. 31, 15-20 (2014). HPTLC of sibutramine in food supplements and soluble beverages on silica gel with toluene - methanol 9:1. Quantitative determination by absorbance measurement at 225 nm. The hRF value for sibutramine was 53. Linearity was in the range of 78-3020 ng/zone. The method was sensitive to detect 0.3 mg of sibutramine per capsule of food supplement and showed comparable results with those obtained with HPTLC-UV and HPLC-MS.

Braz. J. Microbiol. 44, 401-406 (2013). HPTLC of trichothecene in multiplex PCR isolates of Fusarium culmorum on silica gel with chloroform - methanol - water 45:5:1. Detection by dipping into 10 % aluminium chloride in methanol-water mixture, followed by heating at 110 ºC for 20 min. Qualitative determination at UV 366 nm._x000D_