J. Planar Chromatogr. 22, 277-281 (2009). HPTLC of racecadotril (2-{2(acetylsulfanylmethyl)-3-phenylpropanoyl}amino acetic acid benzyl ester) and its degradation products in the bulk drug and in a pharmaceutical formulation on silica gel with n-hexane - ethyl acetate 7:3 in a twin trough chamber saturated for 30 min. Quantitative determination by absorbance measurement at 230 nm. The limit of detection and quantification was 50 and 100 ng/band, respectively.

Abstract No. F-236, 61st IPC (2009). HPTLC of pantoprazole and mosapride citrate on silica gel (pre-washed with methanol) with ethyl acetate - benzene - methanol - 25 % ammonia 48:35:15:2 at room temperature. Quantitative determination by absorbance measurement at 250 nm or 276 nm. The method was linear in the range of 3-7 µg/band for both drugs. Recovery was between 99.0 and 103.5 %.

Abstract No. F-264, 61st IPC (2009). HPTLC of pantoprazole and itopride hydrochloride on silica gel with n-butanol - chloroform - 25 % ammonia 7:2:1. The hRf value was 54 and 75 for pantoprazole and itopride hydrochloride, respectively. Quantitative determination by absorbance measurement at 291 nm. The linearity of the method was 80-240 ng/band for pantoprazole and 300-900 ng/band for itopride.

J. Chinese Trad. & Herb. Drugs 40 (6), 900-903 (2009). TLC of the TCM drug extracts on silica gel with 1) ethyl acetate - formic acid - glacial acetic acid - water 15:1:1:2; 2) petroleum ether (30-60 °C) - ethyl acetate - formic acid 15:5:1; 3) chloroform - ethyl acetate - methanol - water 31:81:25:2. Detection 1) by spraying with 10 % sulfuric acid in ethanol followed by heating at 105 °C until coloration; 2) under UV 254 nm.

J. Liq. Chromatogr. Relat. Technol. 33, 778-785 (2010). TLC of ofloxacin and pefloxacin on silica gel with acetonitrile - formic acid - water 40:3:7 in chamber saturated for 30 min. Quantitative determination by densitometry at 295 nm for ofloxacin and at 280 nm for pefloxacin. The regression equations were achieved using computer program STATISTICA 7.1.

J. Planar Chromatogr. 23, 75-78 (2010). HPTLC of fluphenazine hydrochloride on silica gel (prewashed with methanol) with methanol - water 9:1 in a saturated twin-trough chamber. Quantitative determination by absorbance measurement at 306 nm. Linearity was in the range of 100 to 500 ng/µL with a correlation coefficient of 0.998. LOD and LOQ were 1.45 and 4.40 ng/zone, respectively. Intra-assay and inter-assay precision, expressed as relative standard deviation (RSD), were in the range 0.73-1.77 % (n = 3) and 1.18-1.86 % (n = 9), respectively. Recovery of fluphenazine hydrochloride was between 98.3 and 101.5 %, with RSD not higher than 1.87 %. The method was selective for fluphenazine hydrochloride and the preservatives in the injections.

Abstract No. C-454, 61st IPC (2009). HPTLC of ursolic acid and luteolin from aerial parts of Lippia nodiflora on silica gel with toluene - ethyl acetate - formic acid 70:30:3. The hRf value of luteolin was 34 and of ursolic acid 85. Densitometric analysis at 254 nm for luteolin and at 530 nm for ursolic acid after derivatization with natural products reagent followed by PEG reagent. The recovery of both marker components was in the range of 98.6-100.5 %.

Analytical Chemistry - An Indian Journal 8(1), 29-33 (2009). A sensitive and accurate HPTLC method has been developed for the determination of eugenol from the leaf and berry powder of Pimenta dioica (L) Merr. The leaf and berry powders were extracted with methanol. HPTLC on silica gel with ethyl acetate - methanol - water 6:2:1. Detection and quantification by densitometry at 280 nm. Linear response to eugenol was found to be in the concentration range of 200-600 ng/band. The developed method can be used for routine quantitative monitoring of eugenol from the dried leaf and berry powder of Pimenta dioica (L) Merr.