J. Planar Chromatogr. 24, 150-153 (2011). HPTLC of tolterodine in the bulk drug on silica gel with toluene - methanol 1:1 + 1 drop aqueous ammonia in a saturated twin-trough chamber lined with filter paper; optimum saturation time was 30 min. The hRf value was 40. Quantitative determination by densitometry in absorbance mode at 284 nm. Linearity was between 200 and 1800 ng/band (r² >0.990). The limits of detection and quantification were 100 and 200 ng/band, respectively. The repeatability and intermediate precision (%RSD, n = 6), was between 0.7-0.9 % and 0.6-1.8 %, respectively. Mean recovery was 100.9 %.

J. Planar Chromatogr. 24, 140-144 (2011). HPTLC of sertraline hydrochloride on silica gel, prewashed with methanol, with toluene - ethyl actate - ethanol - ammonia 80:20:95:1 in a twin-trough chamber saturated for 30 min at 25 +/- 2 °C. The hRf value was 33. Quantitative determination by densitometry in absorbance mode at 273 nm. The linearity was in the range of 2-12 µg (r² = 0.9996). The intra-day and inter-day precision for sertralin hydrochloride standard was 0.6, 0.4, 0.3 % and 0.8, 0.4, 0.2 %, respectively; for sertraline hydrochloride in commercial tablets 0.6, 0.2, 0.1 % and 0.6, 0.3, 0.2 %, respectively. The LOD was 670 ng/band and the LOQ 710 ng/band.

Planta Med. 74, 1397-1402 (2008). Analytical and preparative TLC of three steroidal saponins (25R)-spirost-5-ene-3ß, 17alpha-diol (pennogenin), 3-O-{O-alpha-L-rhamnopyranosyl-(1-2)-O-[O-ß-xylopyranosyl-(1-5)-alpha-L-arabinofuranosyl-(1-4)]-ß-D-glucopyranoside} on silica gel with dichloromethane - methanol - water 80:19:1 or 70:29:1. Detection by spraying with phosphomolybdic acid. Additional detection by antifungal bioassays.

J. Planar Chromatogr. 24, 373-375 (2011). HPTLC of red clover capsule extracts and formononetin, biochanin A, daidzein, glycitein, and genistein on silica gel, prewashed with methanol, with dichloromethane - glacial acetic acid - ethyl acetate 12:2:1 in a horizontal chamber saturated for 15 min. Quantitative determination by densitometry at 260 nm. The hRf value was 29, 34, 41, 48, and 59 for daidzein, glycitein, genistein, formononetin, and biochanin A, respectively. The two major isoflavones are formononetin and biochanin A. The limit of detection and quantification was 14 and 47 ng/band for formononetin and 12 and 40 ng per band for biochanin A, respectively. The recovery was 93.3-100.7 % for formononetin and 102.0-109.4 % for biochanin A.

Acta Chromatographica 22 (4), 651-663 (2010). HPTLC of wedelolactone (WED) and asiaticoside (ASI) in Eclipta alba and Centella asiatica Linn., respectively, on silica gel with toluene - acetone - methanol - formic acid 60:40:40:1. The hRf value was 26 and 75 for ASI and WED, respectively. Detection by spraying with 10 % methanolic sulphuric acid. Quantitative evaluation by absorbance measurement at 317 nm for WED and at 530 nm after derivatization for ASI. The linearity was in the range of 50-250 ng/band for WED and 150-550 ng/band for ASI with r = 0.999 for WED and 0.9989 for ASI. The recovery of WED was 99.3 % and of ASI 99.5 %.

CBS 107, 2-4 (2011). To ensure effective cleaning of the reaction vessels in pharmaceutical production wool swabs are used to swipe a defined area of the vessel. The wool swabs are extracted with chloroform and the extracts are applied as rectangles of 4x3 mm. HPTLC on silica gel with toluene - ethyl acetate 3:2 with chamber saturation for 10 minutes. Identification of substances by densitometric spectra recording between 200-350 nm followed by 3-level calibration. Detection by spraying or immersion in methanol - sulfuric acid 9:1 and heating for 5 min at 105 °C. Evaluation under visible light and UV 366 nm.

Indian Drugs 48(4), 49-55 (2011). HPTLC of hydrochlorothiazide (HCZ), amlodipine besylate (AMB) and valsartan (VAL) on silica gel with ethyl acetate - methanol - 10 % ammonia 17:4:2. The hRf value was 26 for AMB, 34 for VAL and 82 for HCZ. The linearity range was 100-700 ng/band for VAL and HCZ and 50-400 ng/band for AMB.

J. Planar Chromatogr. 23, 365-368 (2010). HPTLC of palmitoyl hexapeptide (an antiwrinkle peptide) on silica gel with toluene - ethanol 9:1 in a twin-trough chamber with saturation for 30 min at room temperature (25 +/- 2 °C). The hRf was 33. Quantitative determination by absorbance measurement at 211 nm. Linearity was between 10 and 30 ng/band. The LOD and LOQ was 3 and 9 ng/band, respectively. The intra-day precision (%RSD, n = 6) was 0.9-1.5 % and the inter-day precision 0.9-1.4 %. The small %RSD obtained after small changes of the method conditions indicate the method is robust. The recovery of the method was in the range of 98.9-101.3 %.