Brazilian Journal of Pharmacognosy. 23, 207-216 (2013). HPTLC fingerprint of four similar looking Adiantum species: A. capillus-veneris L. (1), A. lunulatum Burm. f. (2), A. peruvianum Klotzsch (3), and A. venustum D. Don. (4) on silica gel with toluene - ethylacetate 4:1. (1) could be clearly differentiated from other species by the presence of characteristic blue colored bands at hRf 57 under UV 366 nm. (2) and (3) shared additional bands at hRf 22 and 27 under UV 366 nm and at hRf 74 under UV 254 nm. A characteristic band at hRf 41 was observed only in (3) under UV 366 nm.

Acta of Trad. Chinese Med. & Pharm. 40 (3), 100-102 (2012). Ruxian pills are a herbal TCM for treatment of abdominal mass accumulation and breast tumors etc. The medicine is prepared by blending the decoction and powders of the component drugs with honey in proper proportion and then processing into pills. For quality control, TLC on silica gel 1) for Radix Angelicae Sinensis, with n-hexane – ethyl acetate 9:1, detection under UV 366 nm; 2) for Radix Paeoniae Rubra, with chloroform – ethyl acetate – methanol – water 200:25:50:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:25 and heating at 105 °C, viewing in daylight; 3) for Spina gleditsiae, with toluene – ethyl acetate – formic acid 7:2:1, detection under UV 366 nm. Using this method the optimum extraction conditions for preparing the decoction from the crude drugs as well as the stability during storage of the final preparation were studied.

by thin-layer chromatography) (Chinese). J. of Guizhou Normal Univ. (Natural Sci.) 30 (3), 9-11 (2012). Emilia sonchifolia (Linn.) DC. is a TCM herb used in the formulations for treating conjunctivitis, tonsillitis, pneumonia, infectious hepatitis, dysentery, diarrhea, urinary tract infection, etc. For quality control TLC of the herb extracts an the standard emodin on silica gel with chloroform – methanol – formic acid 300:10:1, detection 1) under UV 366 nm; 2) by exposing to ammonia vapors and viewing under UV 366 nm.

bispinosa Roxb. (Singhara). J. Planar Chromatogr. 26, 316-321 (2013). HPTLC of gallic acid (1), caffeic acid (2), quercetin (3) and kaempferol (4) in the green, red, and black fruits of Trapa natans var. bispinosa Roxb. (Singhara) on silica gel with toluene - ethyl acetate - formic acid 13:11:2. Quantification by absorbance measurement at 282 nm. The hRf values for compounds (1) to (4) were 23, 34, 38 and 46, respectively. Linearity was in the range of 1000-1500 ng/zone for (1) to (4). LOD and LOQ were 116 and 351 ng/zone for (1), 135 and 409 ng/zone for (29, 92 and 278 ng/zone for (3) and 87 and 263 ng/zone for (4), respectively. Average recoveries were 98.5 % for (1), 98.2 % for (2), 96.4 % for (3) and 97.3 % for (4). Intermediate/interday/intra-day precision was below 3 % (n=6).

J. Planar Chromatogr. 26, 370-374 (2013). HPTLC of ciprofloxacin in tablets on silica gel with acetone - water - ammonia 30:3:5. Quantification by absorbance measurement at 280 nm. The hRf of ciprofloxacin was 27. Linearity was in the range of 250-600 ng/zone. Recovery was in the range of 96-101 %. Intermediate/interday/intra-day precision was below 2 %.

Chinese J. of Med. Guide 11 (1), 470-472 (2013). Shenshi Keli granule is a herbal TCM preparation for the treatment of urinary calculus. For quality control TLC on silica gel 1) for Dichondra repens Forst., with chloroform – acetone 19:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C for 5 min and viewing under UV 366 nm; 2) for Chicken gizzard’s stomach lining, with n-butanol – glacial acetic acid – water 7:1:1, detection by spraying with 0.3 % ninhydrin in n-butanol and heating at 105 °C until the zones are visible in daylight.

and Pterocephalus hookeri (Clarke) Hoeck by thin-layer chromatography) (Chinese). Chinese J. of Ethnopharm. 7 (7), 19-21 (2013). The roots of the grasses Nardostachys chinensis Batal. and Pterocephalus hookeri (Clarke) Hoeck are herbal Tibetan drug for treating epidemic disease, long fever and dysentery. During investigation of the medicinal plants growing in the Qinghai and Tibetan Plateau the following identification method for the two drugs was developed. TLC on silica gel 1) for Nardostachys chinensis Batal., a) with cyclohexane – ethyl acetate 5:1 and b) with petroleum ether (60-90 °C) – acetone 4:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones are visible in daylight. With mobile phase a) 3 zones were separated and with b) 6 zones; 2) for Pterocephalus hookeri (Clarke) Hoeck, a) with benzene – ethyl acetate 16:7 and b) with petroleum ether (60-90 °C) – acetone 3:2, all detection under UV 366 nm. With both mobile phases three blue fluorescent zones were detected.

Chinese J. of Henan Univ. (Med Sci.) 32 (2), 117-121 (2013). Shiming Granule is a herbal TCM preparation for invigorating blood circulation, improving liver and pulmonary functions and the eyesight. For quality control, TLC on silica gel 1) for Rhizoma et Radix Notopterygii, with n-hexane – ethyl acetate 7:3, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating until the zones are visible in daylight and under UV 366 nm; 2) for Bupleurum chinense, with ethyl acetate – methanol – water 8:2:1, detection by spraying with 2 % p-dimethylaminobenzaldehyde in 40 % sulfuric acid and heating at 60 °C, viewing under UV 366 nm; 3) for Rhizoma Atractylodis, with petroleum ether (60-90 °C) - ethyl acetate 20:1, detection by spraying with 5 % p-dimethylaminobenzaldehyde in 10 % sulfuric acid and heating until the zones are visible in daylight; 4) for Exocarpium Citri Rubrum, with ethyl acetate – methanol - water 100:17:10, detection by spraying with 3 % aluminiumchloride in ethanol, viewing under UV 366 nm; 5) for Radix Saposhnikoviae, with chloroform – methanol 4:1, detection under UV 254 nm; 6) for Salvia miltiorrhiza, with benzene – ethyl acetate 19:1, detection under UV 366 nm; 7) for Radix Paeoniae Rubra, with chloroform – methanol – formic acid 200:25:50:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating until the zones are visible in daylight; 8) for Herba Equiseti Hiemalis, with cyclohexane – ethyl acetate – formic acid 20:10:1, detection by spraying with 5 % aluminium chloride in ethanol and viewing under UV 366 nm; 9) for Cuscuta chinensis Lam., with ethyl acetate – butanone – formic acid – water 11:1:1:1, detection by spraying with 5 % aluminium chloride in ethanol and heating at 105 °C until the zones are visible, and viewing under UV 254 nm; 10) for Rehmannia glutinosa Libosch, with chloroform – methanol – ammonia 40:10:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C until the zones are visible in daylight; 11) for Rhizoma Atractylodis Macrocephalae, with cyclohexane – ethyl acetate 7:3, detection by spraying with 5 % p-dimethylaminobenzaldehyde in sulfuric acid – ethanol 1:10 and heating at 105 °C until the zones arevisible in daylight; 12) for Radix Glycyrrhizae, with ethyl acetate – formic acid – water 15:3:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing under UV 366 nm; 13) for Radix Achyranthis Bidentatae, with chloroform – methanol 30:1, detection by spraying with 5 % phosphomolybdic acid in n-propanol and heating at 110 °C until the zones are visible in daylight.