J. Chromatogr. A 1084 (1-2), 13-17 (2005). A variant of electroosmotic thin-layer chromatography is suggested with the use of low volatility compounds as mobile phases aimed at drastically decreasing the evaporation of the mobile phase and improving the reproducibility of the method. The linear movement velocity of zones of separated compounds is experimentally shown to increase 2- to 12-fold in electroosmotic chromatography (compared to similar values in traditional TLC). The separation efficiency is also considerably increased.

J. AOAC Int. 88, 1544-1548 (2005). TLC of antimycotics (bifonazole, clotrimazole, and miconazole) and preservatives (benzyl alcohol, benzoic acid) on silica gel with 1) ethyl acetate - n-heptane - methanol - diethylamine 30:45:10:2 for bifonazole and benzyl alcohol; 2) n-butyl acetate - n-heptane - methanol - diethylamine 30:45:10:2 for clotrimazole and benzyl alcohol; 3) n-butyl acetate - carbon tetrachloride - methanol - diethylamine 6:12:5:1 for miconazole and benzoic acid. Quantitative determination by reflectance/absorbance measurement at 230 nm (bifonazole, benzyl alcohol, miconazole, and benzoic caid) and at 210 nm (clotrimazole and benzyl alcohol). Recovery rates for all substances ranged from 98.7 to 100.7 %. The limits of detection and quantitation were 0.03 to 0.2 µg and 0.1 to 0.5 µg/spot, respectively.

J. AOAC Int. 88, 1517-1524 (2005). HPTLC of fenofibrate and gemfibrozil on diol phases in horizontal chambers using the sandwich technique with hexane - tetrahydrofuran 4:1. Quantitative determination by classical densitometry at 227 nm and videodensitometry at 254 nm. Recovery in the densitometric assay was 101.4 % for fenofibrate and 100.5 % for gemfibrozil. Videodensitometry resulted in recoveries of 102.7 % and 98.8 %, respectively.

J. Planar Chromatogr. 18, 460-464 (2005). HPTLC of ethamsylate and mefenamic acid on silica gel prewashed with methanol, in a pre-saturated twin-trough chamber with chloroform - methanol - acetic acid 50:40:1. After drying at 50 °C for 5 min quantitative determination by absorbance measurement at 300 nm. The validated calibration range was 500-2500 ng/spot (r = 0.998) and 500-2500 ng/spot (r = 0.997) for ethamsylate and mefenamic acid, respectively.

J. Liq. Chrom. & Rel. Technol. 26, 3453-3462 (2003). HPTLC of caffeine and acetaminophen on silica gel in a saturated twin-trough chamber with ethyl acetate - glacial acetic acid 19:1. Quantification at 254 nm. Diphenhydramine, pseudoephedrine, and acetaminophen were well separated from the caffeine zone. Precision (relative standard deviation) was 1.19 %; limit of detection was 0.2 µg for caffeine and 0.08 µg for acetaminophen; precision of duplicate samples (RSD) ranged from 0.95 to 7.56 %.

Indian J. Pharm Sciences 67 (6), 762-764 (2005). HPTLC of fenofibrate in methanolic capsule extracts on silica gel with toluene - chloroform 7:3 with chamber saturation for 10 min. Quantitative determination by absorbance measurement at 296 nm. Linearity range was 1.2-3.8 g with recovery of 101.43 %. The proposed validated method was stability indicating and useful for routine analysis.

Indian Drugs 42 (7), 465-468 (2005). HPTLC of tizanidine and diclofenac in tablet formulations on silica gel with chloroform - methanol 4:1. Quantitative determination by absorbance measurement at 230 nm. Cetrizine was used as an internal standard. The solvent system was found to give compact spots for diclofenac sodium (Rf value 0.86), tizanidine (0.26) and cetrizine (0.52). The method was validated for linearity, accuracy and precision. Linearity for tizanidine was 0.6-1.4 µg/mL, and for diclofenac sodium 7.5-17.5 µg/mL . The mean recoveries obtained for tizanidine and diclofenac sodium were 98.73 % and 99.70 %, respectively. The proposed method was accurate, precise, selective and rapid for simultaneous estimation of tizanidine and diclofenac sodium in tablets.

J. Planar Chromatogr. 19, 401-404 (2006). TLC of methionine, L-cystine, calcium pantothenate, vitamin B1, vitamin B7, and p-aminobenzoic acid on silica gel in a pre-saturated chamber with n-propanol - water - chloroform 5:2:1. Quantification of methionine was based on the oxidation and reaction with leuco xylene cyanol FF solution, which formed a blue dye. Quantitative determination by absorbance measurement at 613 nm.