Abstract No. C-231, 61st IPC (2009). An HPTLC method is reported for determination of 3-OH-androstane-(16,17-C) (6-methyl-2-1-hydroxy-isopropene-1-yl)-4,5,6 H-pyran, a phyto constituent of Eugenia jambolana. The compound was isolated by ethanolic extraction, identified by melting point, IR, and NMR, and used as marker. HPTLC on silica gel with toluene - ethyl acetate 17:3. Densitometric evaluation at 366 nm. The method was linear in the range of 1000-5000 ng/band. It can be used for routine quality control of Eugenia jambolana seeds and herbal formulation.

Abstract No. C-453, 61st IPC (2009). Fingerprint analysis of budmunchiamines, the main constituents in Albizia amara. Dried powdered leaves were extracted with petroleum ether (60-80 °C), chloroform, ethyl acetate and 90 % methanol by maceration for 48 h. TLC on silica gel with chloroform - methanol 19:1. Zones 2, 4 and 8 corresponded to the marcocylic alkaloids budmunchiamine A, B, and C, which was confirmed by FTIR, NMR and MS.

Encyclopedia of Chromatography Third Edition 1, 2111-2115 (2009). The author describes the sample preparation methods prior qualitative and quantitative analysis by TLC and HPTLC. Classical extraction and clean-up methods include liquid-liquid extraction and soxhlet extraction. Modern methods such as solid phase extraction (SPE), pressurized liquid extraction (PLE), supercritical fluid extraction (SFE) and immunoaffinity (IA) extraction and clean-up are also described.

Indian J. Pharma. Sci. 70(4), 529-531 (2008). HPTLC on silica gel with ethyl acetate - acetone - acetic acid 13:6:1 with chamber saturation for 15 min. Densitometric evaluation at 208 nm. The hRf value was 51 for quinapril hydrochloride and 76 for hydrochlorothiazide. The method was linear in the concentration range of 400-2800 ng/zone for quinapril and 500-3500 ng/zone for hydrochlorothiazide. The recovery was in the range of 98.3-100.8 %.

Analytical Chemistry - An Indian Journal 9(1) (2009). An HPTLC method has been developed for simultaneous estimation of paracetamol and ibuprofen. HPTLC on silica gel with ethyl acetate - acetone - n-butanol - 25 % ammonia 3:4:3:1. Densitometric quantification at 254 nm. The hRf value of ibuprofen was 32 and of paracetamol 86. The method was linear in the range of 120-600 ng/band for paracetamol and 130-650 ng/band for ibuprofen.

obtained by different techniques. J. Planar Chromatogr. 23, 320-322 (2010). HPTLC of thymol in Satureja hortensis L. extracts on silica gel with toluene - ethyl acetate 93:7. Detection by spraying with anisaldehyde reagent. Quantitative determination by absorbance measurement at 513 nm. The quantity of thymol extracted depends on the composition of the extraction solvent and the extraction technique. The best results were obtained by maceration with ethanol.

E-Journal of Chemistry 7(2), 341-348 (2010). HPTLC of nebivolol hydrochloride and hydrochlorothiazide on silica gel with ethyl acetate - methanol - 25 % ammonia 17:2:1 with chamber saturation. The hRf value of hydrochlorothiazide was 21 and of nebivolol 41. Densitometric evaluation at 285 nm. The method was linear in the range of 200-1000 ng/band for nebivolol and 500-2500 ng/band for hydrochlorothiazide. The recovery was 98.9-102.4 %. Comparison of the HPTLC method with a RP-HPLC method and a UV spectroscopic method gave comparable results. The HPTLC method is suitable for routine quality control.

Journal of Natural Remedies 10(2), 170-174 (2010). The powdered plant material was defatted, extracted with methanol, concentrated and successively extracted with ethyl acetate and n-butanol. The resulting fractions were concentrated and subjected to chromatographic fingerprint analysis of the phytoconstituents, i.e. alkaloids, saponins, tannins, flavonoids, anthraquinones and sterols. TLC on silica gel with n-butanol - acetic acid - water 4:1:5 for the ethyl acetate fraction and with chloroform - methanol 9:1 for the n-butanol fraction. Densitometric evaluation at 254 nm and 366 nm for the n-butanol fraction and at 290 nm for the ethyl acetate fraction. Derivatization with vanillin-sulfuric acid reagent for the n-butanol fraction and with AlCl3 reagent for the ethyl acetate-fraction, evaluation at 600 nm. The n-butanol fraction contained sterols and saponins, the ethyl acetate fraction flavonoids.