IPA Convention, 2010, RA-PO 26. HPTLC of risedronate sodium on silica gel with water - methanol - 25 % ammonia 20:3:3. Densitometric evaluation at 262 nm. The method was linear in the range of 3-6 ng/band, recovery was 98.6 %. Results were comparable with HPLC results. The advantage of the HPTLC method is the simultaneous analysis of several samples.

J. Liq. Chromatogr. Relat. Technol. 31, 2429-2441 (2008). TLC of citalopram, sertraline, fluoxetine, and fluvoxamine on silica gel with acetone - benzene - 25 % ammonia 10:9:1 in a twin-trough chamber saturated for 15 min. Detection under UV 254 nm. The hRf value was 28 for fluoxetine, 44 for citalopram, 56 for fluvoxamine, and 68 for sertraline (with a standard deviation less than 0.02 % in all cases). Quantitative determination by absorbance measurement at 240 nm. The calibration curve was linear in the range of 500-5000 ng/spot for all analyzed compounds; correlation coefficients were found to be more than 0.998 for all drugs (except the 0.991 for fluvoxamine). LOD was 40 ng/spot for citalopram and 50 ng/spot for fluoxetine, fluvoxamine, and sertraline, respectively. LOQ was found to be 130 and 160 ng/spot for citalopram and fluoxetine, respectively. Intra-assay precision (% RSD) was within the range of 0.52-0.87 % and 1.34-1.84 % for citalopram and fluoxetine, respectively, inter-day precision for the analyses conducted on three consecutive days was below 1.8 % and 2.5 % for citalopram and fluoxetine, respectively. The recoveries (RSD) of citalopram ad fluoxetine were found to be in the range of 99.3-100.3% (0.8 %) and 99.4-100.4 % (1.9%), respectively.

Abstract No. C-97, 61st IPC (2009). Chromatographic methods are reported for identification (TLC) and quantification (HPTLC) of withaferin-A in methanolic and chloroform extract of dried fruits of Withania coagulans. Chromatographic separation on silica gel with toluene - ethyl acetate - formic acid 5:5:1. The identification of withaferin-A in both chloroform and methanolic extracts was performed by comparison of hRf values and UV absorbance maxima (209 nm). Quantification was performed by absorbance measurement at 540 nm after spraying the developed plate with Liebermann-Burchard reagent. Methanolic extracts and chloroform extracts contained 3.67 mg/g and 2.10 mg/g of withaferin-A, respectively. No withaferin-A was found in hydroalcoholic extracts.

Encyclopedia of Chromatography Third Edition 1, 514-517 (2009). This review describes the three most important TLC-based drug screening methods for the analysis of the most counterfeited drugs, which are intended to treat tuberculosis infections, macrolides antibiotics, and drugs from the World Health Organization. The methods correspond to the Speedy TLC kit, Fast Chemical Identification System and MiniLab TLC system. The author also describes the advantages of drug-screening TLC methods and its application in the laboratory or in the field.

as a leading compound for quantification in raw material and finished product. J. Liq. Chromatogr. Relat. Technol. 33, 996-1004 (2010). TLC of xylose with acetonitrile - water 9:1 with chamber saturation at ambient temperature. Detection by dipping into 4-aminobenzoic acid reagent for 1 to 2 s followed by heating at 110 °C for 10 min. The hRf of xylose was 56. Quantitative determination by densitometric evaluation at 366 nm. The linearity determination coefficient was r2 = 0.9999. The recovery of xylose was between 102 and 106 %. The precision of a six-time preparation was 1.6 %.

Asian J. Chemistry 22(1), 27-30 (2010). HPTLC of simvastatin (in bulk and tablet dosage form) on silica gel with toluene - ethyl acetate - formic acid 10:3:1. The hRf value of simvastatin was 26. Densitometric evaluation at 242 nm. The method was linear in the range of 200-1000 ng/band.

Analytical Chemistry - An Indian Journal 8(4), 608-612 (2009). An HPTLC method is reported for estimation of lupeol and beta-sitosterol from the whole plant of Vernonia cinerea (Acanthaceae). Methanolic extracts of the plant were subjected to chromatographic analysis on silica gel with toluene - methanol 220:3. Derivatization with Liebermann-Burchard reagent. Densitometric evaluation at 366 nm. The plant was found to contain 0.49 mg/g and 1.4 mg/g of lupeol and beta-sitosterol respectively. The method was suitable for quality control of herbal raw material.

Journal of Pharmacy Research 2(12), 1893-1899 (2009). HPTLC of vasicine and vasicinone in methanolic aqueous extracts of the dried herb and in cough syrup on silica gel with chloroform - methanol 9:1. The hRf value of vasicine was 11 and of vasicinone 45. Densitometric quantification at 280 nm. The method was linear in the range of 2-100 ng/band for vasicine and 25-1000 ng/band for vasicinone. The recovery was 95-102 %. The limit of detection of vasicine was 1 ng and of vasicinone 25 ng. Vasicine and vasicinone were well separated from other constituents of Adhatoda vasica.