J. Planar Chromatogr. 27, 33-37 (2014). HPTLC of alpha-phellandrene (1) and beta-pinene (2) in Anethum graveolens L. on silica gel with ether - dichloromethane 3:7. Detection by spraying with vanillin reagent (15 g vanillin in 250 mL ethanol and 2.5 mL concentrated sulfuric acid), followed by heating at 120 °C. Quantitative determination by absorbance measurement at 600 nm. Linearity was in the range of 40-270 µg/zone for (1) and 100-360 µg/zone for (2). LOD and LOQ were 6.6 and 65.8 µg/zone for (1) and 12.9 and 88.7 µg/zone for (2), respectively. Recoveries were in the range of 95.2-98.8 % for (1) and 97.1-101.4 % for (2). Intermediate/interday/intra-day precision was below 4 % (n=6).

Chinese J. of Henan Univ. (Med Sci.) 32 (3), 184-186 (2013). Mingmu Keli granule is a traditional Chinese compound herbal medicine prescribed clinically to treat impaired vision, red eye and eye dryness, macular degeneration, etc. For quality control, TLC 1) for Rhizoma Atractylodis, on silica gel with petroleum ether (60-90 °C) – ethyl acetate 20:1, detection by spraying with 5 % p-dimethylaminobenzaldehyde in sulfuric acid – water 1:10 and heating mildly until the zones are visible in daylight; 2) for Citrus maxima (Burm.) Merr. cv. Tomentosa, on silica gel first over 3 cm with ethyl acetate – methanol – water 100:17:13, then over 8 cm with toluene – ethyl acetate – glacial acetic acid – water 40:20:3:2, detection under UV 366 nm after spraying with 3 % aluminum trichloride in ethanol; 3) for Saposhnikovia divaricata (Trucz.) Schischk., on silica gel (with fluorescence indicator F254 nm) with chloroform – methanol 8:3, detection under UV 254 nm. Quantification of paeoniflorin by HPLC.

J. Ethnopharmacol. 158, 454-457 (2014). The authors described how the European Pharmacopoeia provides a legal and scientific reference for the quality control of medicines. The document highlighted HPTLC as a tool for the screening of herbal drugs containing certain contaminants, such as aristolochic acids._x000D_

J. Planar Chromatogr. 27, 466-471 (2014). HPTLC of (1) flumethasone pivalate, (2) flumethasone, and (3) clioquinolon on silica gel with benzene – hexane – acetone – formic acid 500:400:200:13. Quantitative determination by absorbance measurement at 235 nm. The hRF values of (1) to (3) were 31, 7 and 64, respectively. Linearity was in the range of 30-400 ng/zone for (1), 50-300 ng/zone for (2) and 150-500 ng/zone (3). The intermediate precision was below 2 % (n=3) for (1) to (3). The LOD and LOQ were 9 and 27 ng/zone for (1), 13 and 39 ng/zone for (2) and 41 and 123 ng/zone for (3). Recoveries for (1) to (3) were in the range of 90-110 %. The method was statistically compared to a validated HPLC method.

J. Ethnopharmacol. 152, 292-301 (2014). HPTLC of extracts of Curcuma longa, Curcuma aromatica and Curcuma zanthorrhiza on silica gel with 1) toluene - acetic acid 4:1 (for curcuminoid determination), 2) acetonitrile - acetone - water 2:2:1 (sugars), and 3) dichloromethane (essential oils). Detection by dipping in anisaldehyde reagent (curcuminoid and essential oil systems) and aniline-diphenylamine-phosphoric acid (sugars) followed by heating at 100 °C, evaluation under white light and UV at 366 nm. Bis-demethoxycurcumin (hRF 18) was only present in Curcuma longa. The HPTLC results were compared with 1H-NMR spectroscopic analyses. The NMR analyses provided a sharper picture than HPTLC but only the combination of both methods enabled the authors of the study to understand both the general variability and the specific differences between the analyzed products.

Chinese J. Mod. Drug Appl. 7 (13), 4-6 (2013). Sangao Tiaohe Wan pill is TCM compound for the treatment of high blood pressure, high blood lipids, diabetes mellitus, vasculitis, etc. For quality control, TLC on silica gel (1) for Salvia miltiorrhiza Bunge and tanshinone ll A with benzene – ethyl acetate 19:1, detection under white light; (2) for Panax pseudoginseng, notoginsenoside R1, ginsenoside Rb1 and ginsenoside Rg1 with chloroform - ethyl acetate – methanol – water 15:40:22:10, detection by spraying with 10 % sufuric acid in ethanol and heating at 105 °C until the spots were visible. Quantitation of tanshinone ll A by HPLC.

J. Liq. Chromatogr. Relat. Tech. 38, 381-389 (2015). Review of the materials, techniques and instruments for the detection and quantification of radiolabeled compounds by planar radiochromatography. Special reference is made to sample preparation, stationary phases (including instant TLCD sheets), initial zone application, mobile phases, stationary phase development, and zone detection and quantification.

J. Planar Chromatogr. 28, 268-273 (2015). HPTLC of astragaloside IV (1) and formononetin (2) in dried roots of Radix astragali (Astragalus membranaceus) on silica gel with petroleum ether - n-butanol saturated with water - glacial acetic acid 7:4:8. Detection by spraying with 10 % sulfuric acid ethanol solution, followed by heating at 105 °C. Quantitative determination by absorbance measurement at UV 254 nm. The hRF values for (1) and (2) were 43 and 75, respectively. Linearity was in the range of 1-10 μg/mL. The intermediate precision was below 0.4 % (n=6). Recovery ranged between 98 and 99 %. Compared to HPLC, HPTLC offered a rapid, economic, and sensitive method for detection of compounds.