Abstract No. 9162, IHCB (2009). HPTLC of glycyrrhizin in methanolic (70 %) extracts of Glycyrrhiza glabra on silica gel with chloroform - methanol - water 130:72:15. Quantitative determination by absorbance measurement at 254 nm and at 420 nm after spraying with anisaldehyde reagent. The method was linear in the range of 0.8-3.8 µg/spot. The limit of detection and quantification was 0.16 and 0.52 µg/spot, respectively. Glycyrrhizin was used as bioactive marker for quality control.

60th Indian Pharmaceutical Congress PA-218 (2008). TLC of aceclofenac and paracetamol on silica gel with toluene - isopropylalcohol - ammonia 8:7:1. The hRf value of aceclofenac was 24 and of paracetamol 68. Quantitative determination by absorbance measurement at 254 nm. Linearity was in the range of 25-2000 ng/band with correlation coefficients of 0.9998 for aceclofenac and 0.9996 for paracetamol. The limits of detection and quantification were 25 and 150 ng/zone for aceclofenac and 50 and 200 ng/zone for paracetamol. Both drugs were subjected to acid and alkali hydrolysis, oxidative degradation, and photodegradation. The degradation products were well resolved from the pure drug.

Abstract No. 9966, IHCB (2009). HPTLC of root tuber extracts of Asparagus gonoclados Baker on silica gel with ethyl acetate - methanol - water 15:3:2. Detection by spraying with anisaldehyde reagent. Quantitative determination by absorbance measurement at 425 nm. Shatavarin IV was used as marker.

Ind. J. Pharma. Sci. 8(4), 211-212 (2009). TLC of clopidogrel bisulphate on silica gel with carbon tetrachloride - ethyl acetate - ammonia 50:3:2. Quantitative determination by absorbance measurement at 230 nm. Linearity was between 300 and 1500 ng. In comparison with the labeled claim the amount of clopidogrel in tablets was 99.2 %. The recovery was 99.2 % (via peak area).

60th Indian Pharmaceutical Congress PA-210 (2008). HPTLC of amitriptyline HCl, trifluoperazine HCl, risperidone and alprazolam on silica gel with carbon tetrachloride - acetone - triethylamine 80:20:3. Quantitative determination by absorbance measurement at 250 nm in the range of 50-1200 ng/spot for amitriptyline HCl, 50-1200 ng/spot for trifluoperazine HCl, 100-2400 ng/spot for risperidone, and 25-600 ng/spot for alprazolam. The limit of quantification for amitriptyline HCl and trifluoperazin HCl was 50 ng/spot, for risperidone 100 ng/spot, and for alprazolam 25 ng/spot.

Abstract No. F-252, 61st IPC (2009). HPTLC of tenofovir on silica gel with n-butanol - acetic acid - water 4:1:1. The hRf value was 58. Quantitative determination by absorbance measurement at 260 nm. Linearity was in the range of 120-600 ng/band. The compound was subjected to different stress conditions (acid, alkali, oxidation, photodegradation and thermal) and degradations products were well resolved from the main component.

J. Planar Chromatogr. 22, 287-291 (2009). HPTLC of the biomarkers gallic acid, lupeol, oleanolic acid, and stigmasterol and plant extracts on silica gel with toluene - ethyl acetate - formic acid 5:5:1 for gallic acid and with toluene - ethyl acetate 4:1 for lupeol, oleanolic acid, and stigmasterol in a saturated twin trough chamber. Quantitative determination by absorbance measurement at 272 nm. Detection of oleanolic acid, lupeol, and stigmasterol by dipping in anisaldehyde reagent followed by heating at 110 °C for 5 min. Densitometric evaluation at 652 nm.

Abstract No. 9452, IHCB (2009). HPTLC of capsaicin on silica gel with toluene - ethyl acetate 3:2. The hRf value of capsaicin was 38. Quantitative determination by absorbance measurement at 280 nm. The method was linear in the range of 100-1000 ng/spot. Capsaicin was subjected to different stress conditions (alkali, acid, oxidation, thermal). The method is suitable for separation of capsaicin from its degradation products and can be used to indicate stability.