J. of Chinese Med. 27 (166), 344-345 (2012). Biqiang Bidou flushing fluid is a herbal TCM preparation for treatment of forehead headaches and sinusitis. For quality control, TLC on silica gel 1) for Honeysuckle and the standard chlorogenic acid, with ethyl acetate – formic acid – water 15:12:5, detection under UV 366 nm; 2) for Baical Skullcap, with ethyl acetate – acetone – acetic acid – water 10:4:5:3, detection by spaying with 5 % ferric chloride in ethanol and viewing in daylight.

Chinese J. of Inform. on TCM 17 (12), 48-50 (2010). Fuyanshuan suppository is a herbal TCM preparation for heat clearing, detoxifying, eliminating stagnation, regulating the flow of vital energy and invigorating the circulation of blood. For quality control TLC of the extracts of the preparation 1) for Ajuga becumbens Thunb., on silica gel with cyclohexane - chloroform – ethyl acetate – glacial acetic acid 40:10:16:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 100 °C, evaluation under UV 366 nm or daylight; 2) for Lonicera japonica, on silica gel with chloroform – acetone – formic acid 3:2:1, detection by exposure to daylight for 10 min and viewing under UV 366 nm.

J. Planar Chromatogr. 25, 559-565 (2012). HPTLC of zafirlukast on silica gel with toluene - methanol - acetone 7:2:1. Quantitative determination by absorbance measurement at 240 nm. Linearity was in the range of 50-400 ng/zone. Limits of detection and quantification were 16 and 50 ng/zone, respectively.

J. Planar Chromatogr. 25, 409-414 (2012). HPTLC of two anthocyanins, cyanidin-3-O-rutinoside (1) and cyanidin-3-O-glucoside (2), in the berries of Euterpe oleracea Mart. on silica gel with ethyl acetate - formic acid - acetic acid - water 100:11:11:26. Quantification by absorbance measurement at 520 nm. The hRf of compounds (1) and (2) were 29 and 37, respectively. Linearity was in the range of 100-500 ng/zone. The limit of detection was 30 ng/zone for (1) and 40 ng/zone for (2) and the limit of quantification was 100 ng/zone for both (1) and (2). The intermediate/inter-day/intra-day precision was below 2.8 % (n=3). The average recovery was between 99.8-101.8 %.

J. of Xinjiang Trad. Chinese Med. 30 (3), 70-72 (2012). Compound Xuelian tablet is a herbal TCM preparation prescribed clinically to cure rheumatism. TLC of the extracts of the medicine on silica gel 1) for Saussurea involucrata, with the upper phase of ethyl acetate – formic acid – water 10:1:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones are visible in daylight; 2) for Rhizoma Corydalis, with cyclohexane – chloroform – methanol 10:6:1, detection by exposure to iodine vapors until the zones are visible, evaluation in daylight and under UV 366 nm; 3) for Radix Angelicae Pubescentis, with petroleum ether (30-60 ºC) – formyl acetate – formic acid 15:5:1, detection under UV 366 nm.

Quim. Nova. 35, 541-545 (2012). HPTLC of isoorientin on silica gel with ethyl acetate - formic acid - water 9:1:1. Detection by dipping into a solution of diphenylboric acid-2-aminoethyl ester (100 mg) and PEG 400 (500 mg) in methanol (10 mL). Quantitative determination by fluorescence measurement at 366 nm. The HPTLC method (120 min) was almost seven times faster than the HPLC method (700 min). The amount of solvent consumed in the HPTLC method (12 mL) was almost eight-fold less than that used in the HPLC method (98 mL), indicating that HPTLC is an alternative technique for analyzing large numbers of samples.

J. Chromatogr. A 1272, 132-135 (2013). Honey is a saturated solution of sugars, used for a long time as a natural source of sugars and is an important ingredient in traditional medicine due to its antimicrobial, anti-inflammatory and antioxidant effects. For quality control and detection of adulteration, TLC of (1) glucose, (2) fructose and (3) sucrose on silica gel aluminum foil twice with ethyl acetate – pyridine – water – acetic acid 12:6:2:1 up to 80 mm. Detection in visible light after dipping in diphenylamine - aniline reagent (2 mg diphenylamine and 2 mg aniline HCl were dissolved in 80 mL acetone, 15 mL phosphoric acid were added and the solution was diluted to 100 mL with acetone) and drying at 110 °C for 15 min. The hRf value of (1) was 46, (2) 52 and (3) 39. Linearity was between 6-16 µg/zone for (1)-(3). Repeatability and intermediate precision (%RSD, n=6) for (1), (2), and (3) was 1.2, 1.3, and 1.1 % and 0.1, 1.3, and 1.1 %, respectively. Recovery (by standard addition) was between 100.7 and 102.1 % for all three substances.

Chinese J. of Lishizhen Trad. Med. & Pharm. 22 (4), 860-862 (2011). Fallopia multiflora (Thunb.) Harald, a medicinal herbal drug widely applied in traditional Chinese medicines, is available from different provinces of China. Presentation of fingerprint methods of Fallopia multiflora from different regions by micellar thin-layer chromatography (MTLC) and IR techniques. MTLC of methanol extracts of Fallopia multiflora and emodin standard solution on polyamide layer with 0.4 % cetylpyridinium chloride in acetone – methanol – 1.0 mol/L NaOH 12:4:5 after chamber saturation for 15 min. Detection of the purple zones in daylight. The ratio of the hRf values of the component zones and those of emodin was calculated. In addition, FTIR of the methanolic extracts and emodin standard solution mixed in KBr tablets. The results of the real life samples available from five different regions are given and proved that the method is suitable for the quality control of the drugs.