J. Planar Chromatogr. 23, 79-83 (2010). HPTLC of anastrozole (2-[3-(1-cyano-1-methyl-ethyl)-5-(1H-1,2,4-triazole-1-yl-methyl)-phenyl]-2-methyl-propanenitrile) on silica gel (prewashed with methanol) with toluene - acetone - ammonia 60:40:3 in a twin-trough chamber previously saturated for 20 min. Quantitative determination by absorbance measurement at 200 nm. Recovery of anastrozole from 1mg tablet formulations ranged from 98.9 to 101.5 %. Intra-day and inter-day precision were 1.34 % and 1.59 %, respectively. LOD and LOQ were 71 and 214 ng/band. The correlation coefficient for the anastrozole calibration was 0.9983 over the range of 500-1500 ng/band (peak area).

Indian Drugs 47(2), 71-74 (2010). HPTLC of irbesartan and hydrochlorothiazide on silica gel with acetone - chloroform - ethyl acetate - methanol 6:6:6:1. The plates were preconditioned for 10 min in a saturated chamber prior to development. The hRf value of irbesartan was 27 and of hydrochlorothiazide 37. The linearity was 1500-9000 ng/band and 125-750 ng/band for irbesartan and hydrochlorothiazide respectively. The average recovery for both drugs was 99.4-99.5 %.

J. Planar Chromatogr. 23, 70-74 (2010). Descripton of a selective and simple HPTLC method for quantification of oenothein B on the basis of the free gallic acid and total gallic acid content after acid hydrolysis. HPTLC of gallic acid on silica gel with benzene - methanol - acetic acid 90:16:8 in a glass chamber previously saturated with the mobile phase vapor for 20 min. Quantitative determination by absorbance measurement at 570 nm after derivatization with 1 % ethanolic iron(III) chloride solution. Average recovery of the active ingredient was in the range 95.4-104.6 %. Linearity was in the range of 440-2200 ng/band. The correlation coefficient r was 0.9991, LOD/LOQ were120/360 ng/band; repeatability (RSD) was 3.0 % and intermediate precision 1.0 %; intraday precision (RSD, n = 6, 440-2200 ng/band) was 3.8 to 5.2 % and interday precision 4.3 to 5.7 %. Both, precision and accuracy, were within acceptable limits for routine drug analysis (</= 15 %).

czern and Brassica nigra L. Asian J. Chem. 22(7), 5349-5354 (2010). An HPTLC method is reported for estimation of sinigrin from seeds of Brassica juncea and Brassica nigra (Cruciferae). HPTLC of methanolic seed extracts on silica gel with ethyl acetate - methanol - water 6:2:1. Densitometric evaluation at 230 nm. The method was linear in the range of 400-1200 ng/band. Amounts of 0.43 and 1.55 % sinigrin were found in Brassica juncea and B. nigra, respectively. The method is suitable for routine quality control of herbal raw material.

Research J. Pharm. and Tech. 3(3), 825-827 (2010). TLC on silica gel (plates pre-washed with methanol) with chloroform - methanol 4:1. The hRf value of aceclofenac was 38 and of diacerein 66. Densitometric evaluation at 256 nm. The linearity range was 10-50 µg/band for aceclofenac and 5-25 µg/band for diacerein. The recovery was in the range of 99.3-101.8 % for both compounds. The method was suitable for routine quality control of combined dosage forms without any interference from the excipients.

J. Chromatogr. Sci. 48(10), 825-829 (2010). TLC of ibuprofen and its impurities in pharmaceutical preparations on silica gel with toluene - ethyl acetate - glacial acetic acid 17:13:1. Quantification by densitometry. The limit of detection and quantification ranges from 0.13-0.27 µg/zone. The recovery is 96.8-99.0 % for the individual constituents. The method is suitable for routine quality-control analysis of pharmaceuticals containing ibuprofen.

Scholars Research Library 2(3), 163-167 (20100. TLC on silica gel with n-butanol - water - methanol - ammonia 8:1:1:2 with chamber saturation for 10 min. The hRf value was 33 for gatifloxacin and 89 ambroxol HCl. The method was linear in the range of 100-600 ng/band for gatifloxacin and 12.5-62.5 ng/band for ambroxol HCI. The recovery was 99.3-100.3 %. Densitometric evaluation at 310 nm.

Indian J. Pharm. Sci. 72(2), 249-252 (2010). HPTLC of rosiglitazone on silica gel with methanol - toluene - chloroform - triethyl amine 2:161:1. Caffeine was used as internal standard. The hRf value of rosiglitazone was 31 and of caffeine 52. Densitometric evaluation at 264 nm. The method was linear in the range of 5-50 µg/band with average recovery of 99.8-100.2 %. The method was suitable for content uniformity testing according to USP and could be used for stability studies as well.