Chinese J. Trad. Pat. Med. (Zhongchengyao) 26 (9), 716-719 (2004). TLC on silica gel with 1) ethyl acetate - chloroform - formic acid 3:2:1; 2) chloroform - methanol 7:2; 3) petroleum ether (60-90 ºC) - ethyl acetate 7:3. Detection 1) under UV 365 nm; 2) by spraying with 10 % H2SO4 in ethanol and heating at 105 ºC for 5 min; 3) by spraying with vanillin - H2SO4 solution. Identification by fingerprint technique. Quantification of icarrin by HPLC.

56th IPC 2004, Abstract No. GP-48. HPTLC of rabeprazole sodium in tablet dosage form on silica gel with ethyl acetate - methanol 9:1. Optimization of experimental parameters such as bandwidth, chamber saturation time, solvent front migration, and mobile phase composition. Quantitative determination by scanning at 260 nm. The Rf value was 0.59. The method was linear with a correlation coefficient of 0.99, recovery was 98.81 %.

Chinese J. Trad. Pat. Med. (Zhongchengyao) 26 (9), App.12-14 (2004). TLC on silica gel with 1) n-butanol - glacial - water 19:5:5; 2) chloroform - methanol - glacial acetic acid - 17:2:1; 3) two fold development with benzene - acetone 9:1. Detection 1) by spraying with ninhydrin solution and heating at 105 ºC; 2) by spraying with 5 % vanillin solution and heating; 3) by spraying with a solution of 8 % vanillin in ethanol - H2SO4, and heating at 105 ºC. Identification by fingerprint technique. Quantification of phyllyrin by densitometry at 280 nm. The quantitative procedure is validated by investigating its linearity (1 - 5 µg/spot, r = 0.9998); precision (RSD = 0.36 % n = 5); repeatability (RSD = 2.92 % n = 5) and standard addition recovery (99.6 %, RSD = 2.4 %, n = 5), etc. The determination results are given for a group of real life samples.

J. Planar Chromatogr. 18, 372-376 (2005). HPTLC of sildenafil in four commercial products and three aphrodisiac herbal preparations on silica gel after pre-saturation with chloroform - methanol - diethylamine 90:10:1. Quantitative determination by absorbance measurement at 305 nm. Recovery was 100.6 and 98.2 % for pure and spiked samples.

J. Chinese Trad. Patent Med. (Zhongchengyao) 27(5), 529-532 (2005). TLC of extracts on silica gel developed with 1) ethyl acetate - ethanol 4:1; 2) ethyl acetate - methyl ethyl ketone - formic acid - water 10:1:1:1; 3) cyclohexane - acetone 10:3. Detection 1) under UV 254 nm; 2) by spraying with 5 % AICl3 in ethanol followed by heating at 105 ºC for 5 min, and inspection under UV 365 nm; 3) by spraying with 5 % vanillin in H2SO4 followed by heating at 105 ºC until the spots are visualized. Identification by fingerprint technique. Quantification of ginsenoside Rg1 by HPLC. The results for three real life samples are given.

J. AOAC Int. 88, 70-79 (2005). HPTLC of haloperidol, amitriptyline, sulpiride, promazine, fluphenazine, doxepin, diazepam, trifluoperazine, clonazepam, and chlorpromazine in 25 selected psychotropic drugs on silica gel with 30 mobile phases, eight of them were selected based on spot location and developing time. Identification and quantification were carried out based on UV densitometric measurements. In addition to retention factors, the absorption spectra recorded directly from chromatograms were also used in qualitative analysis. Limit of detection ranged from 0.009 to 0.260 µg depending on the wavelength used. A satisfying recovery, ranging from 92.9 to 104.7 %, was achieved for individual constituents.

J. Planar Chromatogr. 18, 194-198 (2005). HPTLC of rosuvastatin calcium (with aceclofenac as internal standard) on silica gel with toluene - methanol - ethyl acetate - formic acid 60:10:30:1. Quantitative determination by absorbance measurement at 265 nm. A good determination coefficient (r2 = 0.9999) was obtained for the linearity in the range of 1.0 to 15.0 µg of sample. For formulation and bulk drug the mean percentage assay was 100.09 +/- 0.20 and 100.07 +/- 0.48, respectively. The accuracy of the method was found to be 100.62 % and precision was found to vary from 0.01 to 0.77 %.

Indian Drugs 42 (6), 340-344 (2005). HPTLC of captopril and hydrochlorothiazide in tablets on silica gel with methanol - toluene - ethyl acetate - glacial acetic acid 2:12:6:1. Quantitative determination by absorbance measurement at 219 nm. The Rf value of hydrochlorothiazide was 0.38 and of captopril 0.57. The calibration curve response was 4-14 µg for both drugs. Recovery was determined by standard addition method. The percent recovery by area was found to be 100.25 for captopril and 99.98 for hydrochlorothiazide. The method was suitable for routine quality control of such formulations.