Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

  • Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
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      119 109
      Separation of pigment formulations by high-performance thin-layer chromatography with automated multiple development
      Constanze STIEFEL, Sylvia DIETZEL, M. ENDRESS, Gertrud E. MORLOCK* (*Justus Liebig Univ. Giessen, Chair of Food Sci., Inst. of Nutritional Sci., and Interdisciplinary Res. Center (IFZ), Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany)

      J. Chromatogr. A 1462, 134-145 (2016). Development of simple method for the separation of different colored pigment formulations used in the printing materials on food packaging to control the quality and safety of the package. HPTLC on silica gel by automated multiple development with a 9-step gradient based on ethyl acetate, methanol and water, and ending with toluene. Good resolution of differently soluble constituents of the pigment formulation like additives and coating materials. The results obtained by multi-detection allowed a first assignment of the differently detectable bands to particular chemical substance classes, enabled the comparison of different commercially available pigment batches and revealed substantial variations in the composition of the batches. Characterization of single unknown pigment constituents by HPTLC-MS and HPTLC combined with ATR-FTIR. The new HPTLC method for routine quality control for incoming pigment batches and monitoring of internal pigment production processes secures a consistent pigment composition, resulting in consistent ink quality. Hyphenation of HPTLC with the Aliivibrio fischeri bioassay revealed information on the toxicological potential of different pigment compounds which helps guarantee consumer safety, especially in regard to readily permeable pigment components.

      Classification: 4e, 35d
      120 062
      Qualitative and quantitative characterization of two licorice root species (Glycyrrhiza glabra L
      Débora FROMMENWILER*, V. MAIRE-WIDMER, R. UPTON, J. NICHOLS, G. HEUBL, E. REICH (*CAMAG Laboratory, Sonnenmattstrasse 11, 4132 Muttenz, Switzerland, debora.frommenwiler@camag.com)

      and Glycyrrhiza uralensis Fisch.) by HPTLC, validated by HPLC and DNA sequencing. J. Planar Chromatogr. 30, 467-473 (2017). HPTLC of 18-β-glycyrrhizic acid in two licorice root species (Glycyrrhiza glabra L. and Glycyrrhiza uralensis Fisch) on silica gel with dichloromethane – methanol – water – formic acid 120:75:15:1 with chamber saturation for 20 min to a migration distance of 70 mm. Quantitative determination by absorbance measurement at 254 nm. Detection of species by immersion into sulfuric acid reagent, followed by heating at 100 °C for 10 min, evaluation under UV 366 nm and white light. The HPTLC results correlate with the data obtained by HPLC and by DNA sequencing.

      Classification: 14
      121 018
      Thin-layer chromatographic method of screening the anthocyanes containing alimentary products and precautions taken at the method development step
      E. ?ATA, A. FULCZYK, Teresa KOWALSKA*, M. SAJEWICZ (*Dep. of General Chem. & Chromatogr., Inst. of Chem., Univ. of Silesia, 9 Szkolna Street, 40-006 Katowice, Poland)

      J. Chromatogr. A 1530, 211-218 (2017). Development of a novel and cost-effective TLC method on cellulose (instead of silica gel) for authentication of selected fruit-based alimentary products. As authenticity markers the anthocyanins cyanin chloride, keracyanin chloride, pelargonidin chloride and delphinidin chloride were used. With TLC, the LOD and LOQ for cyanin were of 25 and 75 ng/zone, for keracyanin 55 and 166 ng/zone, for pelargonidin 47 and 140 ng/zone, and for delphinidin 171 and 513 ng/zone. With HPTLC the LOD and LOQ for cyanidin were 107 and 321 ng/zone, for keracyanin 189 and 566 ng/zone, and for pelargonidin 161 and 484 ng/zone (delphinidin was not detectable). Consequently, quantification of anthocyanes in the alimentary products by TLC allowed identification of more target compounds and in a higher number of alimentary products than by HPTLC. (Note that original HPTLC method in J Chromatogr A 1299 (2013) 105-118 was reported to be more sensitive (mainly 3-50 ng/zone) and with higher correlation coefficients of calibration curves (0.9993-0.9999) for 11 anthocyanins/-cyanidins than the HPTLC method that was reproduced in this paper.)

      Classification: 3d, 8
      121 056
      Chromatographic determination of ?-sitosterol, lupeol, and oleanolic acid in Leptadenia pyrotechnica (Forsk
      R. PREET*, R. GUPTA, S. PRADHAN (*Department of Botany, Punjabi University
      Patiala, 147002 Punjab, India, ramanbrar247@gmail.com)

      – a botanical source of the Ayurvedic drug Jivanti. J. Planar Chromatogr. 31, 150-154 (2018). HPTLC of β-sitosterol (1), lupeol (2), and oleanolic acid (3) in Leptadenia pyrotechnica with toluene – ethyl acetate 9:4 for (1); toluene – ethyl acetate – formic acid 70:30:3 for (2), and toluene – methanol – formic acid 45:20:1 for (3). Detection by spraying with freshly prepared p-anisaldehyde sulfuric acid reagent, followed by heating at 110 °C for 3 min. Quantitative determination by absorbance measurement at 530 and 540 nm. The hRf values for (1) to (3) were 64, 84 and 47, respectively. Linearity was in the range of 2-10 μg/zone for (1) to (3). The intermediate precision was below 0.6 %. The LOD and LOQ were 410 and 1270 ng/zone for (1), 550 and 1680 ng/zone for (2) and 300 and 920 ng/zone for (3), respectively. Average recovery for (1) to (3) was 99 %.

      Classification: 13c
      121 082
      Simultaneous determination of acetylsalicylic acid,
      hydrochlorothiazide, enalapril, and atorvastatin in a polypill-based quaternary mixture by TLC
      A. MASLANKA, M. STORLARCZYK, A. APOLA, A. KWIECIEN, U. HUBICKA, W. OPOKA* (*Jagiellonian University Medical College, Faculty of Pharmacy, Department of Inorganic and Analytical Chemistry, 9 Medyczna St, 30-688 Cracow, Poland, anna.maslanka@uj.edu.pl)

      J. AOAC Int. 3, 708-713 (2018). HPTLC of acetylsalicylic acid (1), hydrochlorothiazide (2), enalapril (3), and atorvastatin (4) in a formulation on silica gel with n-hexane – ethyl acetate – methanol – water – acetic acid 42:40:15:2:1. Quantitative determination by absorbance measurement at 210 nm for (3) and 265 nm for (1), (2) and (4). The hRf values for (1) to (4) were 68, 44, 31 and 54, respectively. Linearity was in the range of 0.600-6.000 μg/zone for (1), 0.058-1.102 μg/zone for (2), 0.505-6.560 μg/zone for (3) and 0.100-1.000 μg/zone for (4). The intermediate precision was below 3 % (n=5). The LOD and LOQ were 0.311 and 0.942 μg/zone for (1), 0.043 and 0.130 μg/zone for (2), 0.331 and 1.003 μg/zone for (3) and 0.044 and 0.135 μg/zone for (4). (Note by the editor: Calibration should start at LOQ, not below.) Recovery was between 97.0 and 101.3 % for (1) to (4).

      Classification: 32a
      122 034
      Anticancer activity of saponin isolated from Albizia lebbeck using various in
      vitro models
      T. DESAI*, S. JOSHI (*Department of Pharmacology, Maliba Pharmacy College, Maliba Campus, Bardoli-Mahuva Road Tarsadi, 394350 Gujarat, India, tanvidesai89@yahoo.in)

      J. Ethnopharmacol. 231, 494-502 (2019). HPTLC fingerprint of Albizia lebbeck on silica gel with toluene – ethyl acetate – formic acid 5:5:1. Qualitative identification under UV light at 276 nm. The hRF value for standard catechin was 48. Saponin rich fraction was also analyzed by HPTLC on silica gel with petroleum ether – ethyl acetate 1:1. Detection by spraying with anisaldehyde – sulfuric acid reagent, followed by heating at 110–120 °C for 10–12 min.

      Classification: 8a, 14
      122 055
      Validated high-performance thin-layer chromatographic analysis of ursolic acid and ?-sitosterol in the methanolic fraction of Paederia foetida L
      J. DWIVEDI, A. GUPTA, S. VERMA, M. DWIVEDI, S. PALLWAL, A. RAWAT* (*Pharmacognosy & Ethnopharmacology Division, CSIR?National Botanical Research Institute, Lucknow, India, pharmacognosy1@rediffmail.com)

      leaves. J. Planar Chromatogr. 31, 377-381 (2018). HPTLC of ursolic acid and β-sitosterol in the leaves of Paederia foetida on silica gel with toluene ‒ ethyl acetate ‒ formic acid 80:20:1. Detection by spraying with anisaldehyde–sulfuric acid reagent, followed by heating at 110 °C for 1 min. Quantitative determination by absorbance measurement at 550 nm. The hRF values for (1) and (2) were 22 and 38, respectively. Linearity was between 100 and 500 ng for (1) and (2). LOD and LOQ were 40 and 121 ng for (1) and 50 and 152 ng for (2). The intermediate precision was <2 % (n=3). Recovery ranged from 97.2 % to 99.2 % for (1) and 98.0 % to 99.2 % for (2).

      Classification: 13c
      122 075
      Reversed-phase high-performance liquid chromatography and high-performance thin-layer liquid chromatography methods for simultaneous determination of theophylline, guaifenesin and guaifenesin impurity (guaiacol) in their bulk powders and in dosage form
      E.A. ABDELALEEM, I.A. NAGUIB, S.A. FARAG*, H.E. ZAAZAA (*Anal. Chem. Dep., Faculty of Pharm., Nahda Univ., Beni-Suef, Egypt, shosho_5005eg@yahoo.com)

      J. Chromatogr. Sci., 56 (9), 846–852 (2018). Simultaneous determination of theophylline (THP), guaifenesin (GUI) and guaiacol (GUA, a guaifenesin impurity) by HPTLC on silica gel with ethyl acetate – hexane – methanol - ammonia 65:35:10:2. Detection and quantification by densitometry at 275 nm. The hRf values were 13, 35 and 80 for THP, GUI and GUA, respectively. The linearity range was 0.4–2 μg/band for both THP and GUI, and 0.4–1.2 μg/band for GUA. Validation of the proposed method according to ICH guidelines. Statistical comparison of the results with RP-HPLC showed no difference regarding accuracy and precision.

      Classification: 32c
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