Synthesis and binding studies. J. of Medicinal Chemistry 31, 893-897, (1988). TLC of substituted 4-hydroxy-butyric acids on silica with ethyl acetate - hexane 1:2. Detection by exposing to iodine vapor.

Proc. 6th Int. Symp. Instrum. Planar Chromatogr., (Interlaken 1991), Inst. Chromatogr., Bad Dürkheim, FRG, 37-39 (1991). TLC of fluphenazine-4-chloro-phenoxy-i-butyric ester in sesame oil on silica with two consecutive developments; 1) benzene - acetone 4:5, 2) ethyl acetate- methanol - diethylamine 80:10:5. Visualization under UV 245 nm. Densitometry by absorbance at 260 nm. Standard deviation of the linear calibration curve (0.5 - 3.0 µg) ranged between 2-3%.

TLC in pharmacy: erythromycin. Deutsche Apotheker Zeitung 131, 2710-2711 (1991). TLC of erythromycin on silica with methanol – ethyl acetate 9:1. Visualization by spraying with ammonium-8-anilinonaphthaline-1-sulfonate, heating at 80 °C for some min and under UV 365 nm or by spraying with K2Cr2O7 – sulfuric acid reagent, heating at 90 °C for some min; observation under day light.

J. Planar Chromatogr. 5, 200-204 (1992). TLC of red bell pepper extract and ß-carotene on silica with ethyl acetate - dichloromethane - hexane 24:56:20 (single development); and a variety of solvent combinations (multiple development); scanning at 450 nm (absorbance). The results show a general instability of carotenoids under conditions typically employed in TLC. Rigorous exclusion of oxygen and light would prevent the alteration of carotenoids, however, not particularly easy to achieve in practice. The use of layers impregnated with antioxidant is one possible solution. Great caution should be exercised when reviewing data TLC for the identification and quantification of carotenoids from plant material.

J. Planar Chromatogr. 6, 181-186 (1993). TLC of 30 cephalosporins on silanized silica with 7 mobile phases; detection under UV 254 or by spraying with a solution containing 0.25% ninhydrin and 1% acetic acid in ethanol and subsequent heating at 105 °C for 15 min. Also by exposure to iodine vapor. Identification of all the substances by combining chromatography with two mobile phases and color reactions.

Biochemical Systematics and Ecology 22, 501-505 (1994). Two-dimensional TLC of flavonoids on polyamide with water - butanol - acetone - dioxane 70:15:10:5 for the first development and 1,2-dichloroethane - methanol - butanone - water 55:20:22:3 for the second. Visualization by spraying with 0.1% diphenylboric acid ethanolamine complex in methanol - water 1:1. Examination after 1 h under UV 366 nm.

Eastern Pharmacist 37, 155-156 (1994). HPTLC of paracetamol and metoclopramide on silica (prewashed with methanol) with ethyl acetate - acetone - NH3 60:40:1. Densitometry at 300 nm. Recovery 100-102%.

J. Planar Chromatogr. 7, 458-460 (1994). HPTLC on silica with chloroform - acetic acid - toluene - methanol 8:1:1:1, previously saturated for 1 h. Quantification by densitometry at 366 nm. Detection limit 0.2 ng. A simple, highly sensitive, and fast HPTLC method.