J. Liq. Chromatogr. & Relat. Technol. 29, 2129-2139 (2006). TLC of fusidic acid on silica gel with n-hexane - ethyl acetate - glacial acetic acid 6:3:1. To detect the spots on chromatograms, densitometric measurements at three different wavelengths were carried out, i. e., 240 nm (FA), 260 nm (MHB, PHB), and 290 nm (BHA), leading to increased selectivity and decreased interferences of the peaks.

J. Planar Chromatogr. 20, 423-427 (2007). TLC of 8 essential amino acids (L-lysine, L-valine, L-isoleucine, DL-threonine, L-methionine, L-leucine, DL-phenylalanine, DL-tryptophan) on silica gel with 17 mobile phases, of which the mixed aqueous surfactant solution Triton X-100 (0.001 mM) - sodium dodecyl sulfate, 0.081 mM - acetone 1:1:5 was identified as the best mobile phase for specific separation of lysine. Visualization by spraying with 0.3 % ninhydrin in acetone. Limit of detection was 0.5 µg/zone.

59th Indian Pharmaceutical Congress F-57, 403, (2007). HPTLC of duloxetine hydrochloride on silica gel with chloroform - methanol 4:1. Densitometric evaluation at 217nm. The hRf value of duloxetine was 45. The limit of detection and quantification was 120 and 240 ng/zone, respectively. Degradation products (acid, alkali, oxidative and thermal) were well separated from the main component. The proposed HPTLC method was routinely applied for identification and quantification of the drug in the formulation.

59th Indian Pharmaceutical congress F-173, 431, (2007). HPTLC of famotidine and domperidone on silica gel with ethyl acetate - methanol - toluene - ammonia 40:15:20:2. Densitometry at 285 nm. Linearity was between 100 and 600 ng/µL for both compounds. Limit of detection and quantification was 33 and 100 ng/zone, respectively. Recovery was 99.5 %.

J. AOAC Int. 90, 920-924 (2007) TLC of commercial products containing Aesculus hippocastanum, Turnera diffusa, Matricaria recutita, Passiflora incarnata, and Tilia occidentalis against standardized extracts on silica gel with ethyl acetate - formic acid - acetic acid - water 100:11:11:27 with chamber saturation. Detection by spraying with 1 % methanolic diphenylborinic acid 2-amino ethyl ester, followed by 5 % ethanolic polyethylenglycol 400 (PEG) and visualization under UV light at 365 nm or with anisaldehyde - sulfuric acid reagent followed by heating at 100 - 110°C and visualization under visible and UV light at 365 nm. The standards contained aescin, apigenin-7-glucoside, other glycoside flavonoids, flavonoid aglycones, quercetin, myricetin, kaempferol, and rutin.

J. Planar Chromatogr. 20, 145-147 (2007). HPTLC of clotrimazole and tinidazole on silica gel in a twin-trough chamber with toluene - ethyl acetate - methanol - glacial acetic acid 60:30:10:3 with chamber saturation for 10 min. Quantitation by scanning at 254 nm.

J. Food Comp. Anal. 21, 496-500 (2008). HPTLC of bergenin (1), catechin (2), and gallic acid (3) from Bergenia ciliata and Bergenia ligulata on silica gel with toluene - ethyl acetate - formic acid 4:6:1. Quantitative determination by absorbance measurement at 280 nm. The hRf values of (1), (2), and (3) were 29, 54, and 60, respectively. Selectivity regarding matrix was given. Linearity was between 160 and 800 ng/spot for (1), 160 and 480 ng/spot for (2), and 160 and 560 ng/spot for (3). The limits of detection and quantification were 120 and 160 ng for (1), 80 and 120 ng for (2), and 40 and 80 ng for (3), respectively. Recovery was 99.3 % for (1), 98.6 % for (2), and 99.2 % for (3). The intermediate/interday/intra-day precision (n=6) was 0.04 % and 0.07 % for (1), 0.07 % and 0.06 % for (2), and 0.02 % and 0.11 % for (3).

Ind. J. Pharm. Sci. 69 (6), 834-836 (2007). HPTLC of olmesartan medoxomil and hydrochlorothiazide simultaneously in combined dosage forms on silica gel with acetonitrile - chloroform - acetic acid 14:4:1. Detection at 254 nm. Linearity was between 500 and 750 ng/spot for olmesartan medoxomil and 100 and 600 ng/spot for hydrochlorothiazide. The limit of detection and the limit of quantification for olmesartan medoxomil was 170 and 500 ng/spot respectively, and for hydrochlorothiazide 30 and 100 ng/spot, respectively. The proposed method can be used to determine the drug content of marketed tablet formulation.