J. Liq. Chromatogr. Relat. Technol. 36, 2446-2462 (2013). HPTLC of amitriptyline in tablets on silica gel with acetone - ammonium hydroxide 99:1. Quantitative determination by absorbance measurement at 254 nm. The hRf value of amitriptyline was 46. Linearity was 0.70-1.30 µg/zone. The earlier model TLC to HPTLC-densitometry transfer procedure was expanded by adding sample peak identification confirmation and peak purity (specificity) tests.

collected from different phyto-geographical zones of India. J. Liq. Chromatogr. Relat. Technol. 36, 1810-1821 (2013). HPTLC of protodioscin (1) and prototribestin (2) in fruits of Tribulus terrestris on silica gel with n-butanol - glacial acetic acid - water 40:3:10. Quantitative determination by absorbance measurement at 366 nm. The hRf values of (1) and (2) were 41 and 39, respectively. Linearity was in the range of 100-1000 ng/zone for both (1) and (2). LOD and LOQ were 40 and 100 ng/zone for (1) and 35 and 100 ng/zone for (2). Intermediate precision was below 1.0 %. Recovery ranged 98.0-103.0 % for (1) and 99.1-101.9 % for (2).

J. of China Pharm. 21 (11), 22-24 (2012). Yijingling oral liquid is a herbal TCM preparation for mind and body tranquilization and nourishing the liver and kidney and is prescribed clinically to treat insomnia, amnesia, and the climacteric syndrome. For quality control, TLC on silica gel 1) for Radix Polygoni Multiflori with toluene – ethyl acetate – formic acid 20:2:1, detection by exposing to iodine vapor and viewing in daylight; 2) for Fructus Psoraleae with n-hexane – ethyl acetate 3:1, detection by spraying with 10 % KOH in ethanol and viewing under UV 366 nm.

Shanxi J. of Trad. Chinese Med. 33 (12), 1666-1667 (2012). It is highly debatable whether Radix Aconiti Lateralis Preparata is compatible with Trichosanthes kirilowii Maxim when both needed in processing single formulations. A method is presented to investigate the change of the chemical composition of the extracts obtained by separate and mixed decoction of two kinds of drugs. Preparation of the analytes by decocting, separately, of Radix Aconiti Lateralis Preparata (A), Trichosanthes kirilowii Maxim (B) and combined decoction of the two (C), then extracting, separately, with petroleum ether (30—60 °C) producing extracts A1, B1, C1 and with n-butanol producing extracts A2, B2, C2. TLC on silica gel with the upper phase of n-butanol – ethyl acetate – water 4:1:5, detection 1) under UV 366 nm; 2) by spraying with 10 % sulfuric acid in ethanol and viewing under UV 366 nm. By investigation of 5 batches of the drug sample, significant difference was found between the analytes from single drug decoction and those from mixed drug decoction. The unknown ingredients found in the mixed decoction will be further characterized and analyzed. This study provides an experimental base for exploration of the mechanism of the interaction among the ingredients released from the two drugs.

J. Planar Chromatogr. 26, 73-77 (2013). TLC of guaifenesin (1), pseudoephedrine hydrochloride (2) and guaiacol (3) on silica gel with hexane - ethyl acetate - acetone - water - triethylamine 30:30:40:3:1. Quantitative determination by absorbance measurement at 208 nm for (1) and (2) and 278 nm for (3). The hRf values for (1) to (3) were 34, 12 and 83, respectively. Linearity was in the range of 2-12 µg/zone for (1), 14-25 µg/zone for (2) and 0.1-1.1 µg/zone for (3). LOD and LOQ were established for (3) only: LOD was 40 ng/zone and LOQ 100 ng/zone. Recovery (by standard addition) was between 99.9 and 100.0 %. Intermediate/interday/intra-day precision was below 2 % (n=3).

J. Chil. Chem. Soc. 58, 1663-1666 (2013). HPTLC of rosiglitazone (1) and glimepiride (2) in tablet dosage form on silica gel with methanol - toluene - ethyl acetate 1:8:1. Quantitative determination by absorbance measurement at 228 nm. The hRf values for (1) and (2) were 39 and 20, respectively. Linearity was in the range of 100-1500 ng/zone for (1) and 100-1500 ng/zone for (2). LOD and LOQ were 30 and 35 ng/zone for (1) and 85 and 90 ng/zone for (2), respectively. Recovery was 96 % for (1) and (2). Intermediate intra- and inter-day precision was below 2 % (n=6).

J. of China Pharm. 21 (14), 41-43 (2013). Jinmaxing Zhikepian tablets are a herbal TCM preparation for the treatment of asthma, and acute and chronic bronchitis. For quality control, TLC on silica gel 1) for Peucedanum praeruptorum Dunn, Scutellaria baicalensis and Herbal Ephedrae, with chloroform – methanol – ammonia 40:8:1, detection a) under UV 366 nm and 254 nm, b) by spraying with 2 % ninhydrin in ethanol and heating at 100 °C until the zones are visible in daylight; 2) for Lonicera Japonica, with the upper phase of ethyl acetate – formic acid – water 3:1:1, detection by heating at 100 °C for 5 min and viewing under UV 254 nm; 3) Eriobotrya japonica Thunb., with cyclohexane – ethyl acetate – formic acid 150:40:3, detection by spraying with 10 % sulfuric acid in ethanol and heating at 100 °C until the zones are visible in daylight; 4) for Semen Armeniacae Amarum, with cyclohexane – ethyl acetate – formic acid 150:20:1, detection by spraying with 2 % phosphomolybdic acid in ethanol and heating at 100 °C until the zones are visible in daylight; 5) for Platycodon grandiflorus, with chloroform – ethyl acetate – methanol – ammonia 4:1:4:1, detection by spraying with 5 % ninhydrin in sulfuric acid – ethanol 1:6 and heating until the zones are visible in daylight. Quantification of pseudoephedrine hydrochloride, ephedrine hydrochloride and vitamine B17 by HPLC.

J. of Modern Trad. Chinese Med. 15 (4), 320-323 (2013). Leifengshi Jiaonang capsules are a herbal TCM preparation for the treatment of rheumatism, ankylosing spondylitis, and lupus erythematosus. For quality control, TLC on silica gel 1) for Astragalus membranaceus (Fisch.) Bunge and the standard astragaloside A, with chloroform – methanol – water 13:7:2, detection by spraying with 10 % sulfuric acid in ethanol, heating at 105 °C and viewing in daylight and under UV 366 nm; 2) for Angelica sinensis, with petroleum ether (60-90 °C ) – diethyl ether – acetic acid 20:20:1, detection under UV 366 nm 3) for Epimedium sagittatum, with ethyl acetate – formic acid – butanone – water 10:1:1:1, detection under UV 366 nm. Quantification of ferulic acid by HPLC.