Abstract No. F-311, 61st IPC (2009). HPTLC of levocetirizine dihydrochloride and montelukast sodium on silica gel with chloroform - methanol 93:7. The hRf value was 21 and 65 for levocetrizine and montelukast, respectively. Quantitative determination by absorbance measurement at 345 nm. The method was linear in the range of 100-350 ng/band for levocetrizine and 600-1100 ng/band for montelukast.

Abstract No. 9324, IHCB (2009). HPTLC of hesperidin in orange peel extract and formulation on silica gel with ethyl acetate - methanol - water 100:17:13. Quantitative determination by absorbance measurement at 287 nm. The method was linear in the range of 10-1000 ng/spot. Hesperidin was subjected to degradation studies (acid, alkali, hydrolysis, oxidation, and thermal stress) and was found susceptible to different stress condition. The method was suitable for determination of hesperidin and its degradation products in bulk drug as well as formulations.

Ind. J. Pharma. Sci. 71(1), 72-74 (2009). HPTLC of valsartan and hydrochlorothiazide on silica gel with chloroform - methanol - toluene - acetic acid 60:20:10:1. Quantitative determination by absorbance measurement at 260 nm. The calibration curve was linear between 300 to 800 ng/spot for valsartan and 100 to 600 ng/spot for hydrochlorothiazide. The limit of detection and the limit of quantification for valsartan were 100 and 300 ng/spot, respectively, and for hydrochlorothiazide 30 and 100 ng/spot, respectively.

J. Planar Chromatogr. 22, 273-276 (2009). HPTLC of bicalutamide and leflunomide (as internal standard) on silica gel with toluene - ethyl acetate 4:5 in a twin trough chamber saturated for 30 min at room temperature. Quantitative determination by absorbance measurement at 273 nm. The limit of detection and quantification was 50 and 200 ng/band, respectively.

Talanta 78 (3), 874-884 (2009). Presentation of a sensitive, selective and precise stability-indicating method for the determination of clopidogrel bisulfate in presence of its alkaline degradate and in pharmaceutical formulations. TLC on silica gel with hexane – methanol - ethyl acetate 87:10:3. Quantification by densitometry at 248 nm in the range of 0.6–3 µg/band. Recovery was 99.9 %. Clopidogrel could be determined in the presence of up to 90 % of its alkaline degradate. Method selectivity was evaluated using laboratory prepared mixtures. The analysis of clopidogrel in pharmaceutical dosage forms is possible without interference from additives.

J. Planar Chromatogr. 23, 79-83 (2010). HPTLC of anastrozole (2-[3-(1-cyano-1-methyl-ethyl)-5-(1H-1,2,4-triazole-1-yl-methyl)-phenyl]-2-methyl-propanenitrile) on silica gel (prewashed with methanol) with toluene - acetone - ammonia 60:40:3 in a twin-trough chamber previously saturated for 20 min. Quantitative determination by absorbance measurement at 200 nm. Recovery of anastrozole from 1mg tablet formulations ranged from 98.9 to 101.5 %. Intra-day and inter-day precision were 1.34 % and 1.59 %, respectively. LOD and LOQ were 71 and 214 ng/band. The correlation coefficient for the anastrozole calibration was 0.9983 over the range of 500-1500 ng/band (peak area).

Indian Drugs 47(2), 71-74 (2010). HPTLC of irbesartan and hydrochlorothiazide on silica gel with acetone - chloroform - ethyl acetate - methanol 6:6:6:1. The plates were preconditioned for 10 min in a saturated chamber prior to development. The hRf value of irbesartan was 27 and of hydrochlorothiazide 37. The linearity was 1500-9000 ng/band and 125-750 ng/band for irbesartan and hydrochlorothiazide respectively. The average recovery for both drugs was 99.4-99.5 %.

J. Planar Chromatogr. 23, 70-74 (2010). Descripton of a selective and simple HPTLC method for quantification of oenothein B on the basis of the free gallic acid and total gallic acid content after acid hydrolysis. HPTLC of gallic acid on silica gel with benzene - methanol - acetic acid 90:16:8 in a glass chamber previously saturated with the mobile phase vapor for 20 min. Quantitative determination by absorbance measurement at 570 nm after derivatization with 1 % ethanolic iron(III) chloride solution. Average recovery of the active ingredient was in the range 95.4-104.6 %. Linearity was in the range of 440-2200 ng/band. The correlation coefficient r was 0.9991, LOD/LOQ were120/360 ng/band; repeatability (RSD) was 3.0 % and intermediate precision 1.0 %; intraday precision (RSD, n = 6, 440-2200 ng/band) was 3.8 to 5.2 % and interday precision 4.3 to 5.7 %. Both, precision and accuracy, were within acceptable limits for routine drug analysis (</= 15 %).