J. Pharm. Res. 6(4), 205-207 (2007). HPTLC of itraconazole on silica gel with toluene - acetone - triethylamine 30:30:1. Quantitative determination by absorbance measurement at 270 nm. The hRf value of itraconazole was 62. Linearity was between 200 and 600 ng. The percent drug estimated from the market formulation was found to be 99.6 and 100.2 by peak height and peak area respectively. The percent recovery of the drug (by standard addition method) was between 99.4 and 100.3.

Asian J. Chem. 19(6), 4183-4187 (2007). HPTLC of atenolol and indapamide in tablet formulation on silica gel with toluene - ethanol - acetone - acetic acid 70:25:30:3. Quantitative determination by absorbance measurement at 266 nm. The hRf value of atenolol and indapamide was 21 and 74, respectively. The linearity range was 3.8-10.9 ng/spot and 0.2-0.6 ng/spot for atenolol and indapamide respectively. The recovery was in the range of 98.7-100.1 % for both compounds.

60th Indian Pharmaceutical Congress PA-207, (2008). HPTLC of glibenclamide, rosiglitazone and metformin in combined dosage form on silica gel with methanol - tetrahydrofuran - water - acetate acid 40:9:10:1 with chamber saturation for 10 min. ThehRF values were 56, 60, and 80 for glibenclamide, rosiglitazone, and metformin respectively. Quantitative determination by absorbance measurement at 245 nm. The method was linear in the range of 200-1000 ng/zone (glibenclamide, rosiglitazone) and 120-600 ng/zone (metformin). Recovery was 0.97-99.3 % for all the three compounds. The method was suitable for simultaneous estimation of all three compounds in dosage form.

60th Indian Pharmaceutical Congress PA-87, (2008). HPTLC of carvedilol on silica gel with toluene - chloroform - methanol - acetic acid 20:20:10:1. Densitometric evaluation at 240 nm. The method was linear in the range of 50-1000 ng/spot. The method could effectively separate the drug from its degradation products (acid, base, oxidaline, photodegradation). The kinetic studies confirmed that the drug is more degraded in alkaline medium than in acidic medium.

J. AOAC Int. 91, 1237-1243 (2008). HPTLC of zinc bis(O,O’-diisobutyl dithiophosphate), zinc bis(O,O’-didodecyl dithiophosphate), and Aglamol 99 on RP-2 by automated multiple development with methanol - water - acetic acid 6:3:2 for 25 mm, then acetonitrile - water 11:9 for 60 mm, and again acetonitrile - water for 80 mm, or on silica gel with a 14-step gradient based on toluene. For derivatization, the plate was dipped in a solution of 0.05 % primuline in acetone - water 4:1 for 1 s and immediately dried in warm air. Quantitative determination by fluorescence measurement at 366/>400 nm and by absorbance measurement at 220 nm. HPTLC-ATR-IR and HPTLC-FTIR, as well as HPTLC/DART-MS and HPTLC/ESI-MS were applied for identification.

Anal. Chim. Acta 599 (2), 302-309 (2007). HPTLC of minocycline on silica gel (impregnated with a 10 % (w/v) solution of disodium ethylene diaminetetraacetic acid (EDTA) with a pH of 9.0) with methanol - acetonitrile - isopropyl alcohol - water 10:8:1:1. Quantitative determination by absorbance measurement at 345 nm. The limit of detection was 3.7 ng/spot, recovery was 99.2 - 100.2 %, and precision was good with a %RSD of 0.4 %. The method was able to separate all degradation products (produced by acidic and basic degradation, oxidation and photodegradation) from the pure drug.

Abstract No. 9148, IHCB (2009). HPTLC of gallic acid in Emblica officinalis (an important constituent of Triphala, a popular ayurvedic formulation) on silica gel with toluene - ethyl acetate - formic acid 10:10:3. The hRf value of gallic acid was 45. Quantitative determination by absorbance measurement at 254 nm. The method was linear in the range of 150-530 ng/spot. The extract was found to contain 7.5 mg/g of gallic acid.

J. Chinese Trad. Patent Med., 30 (11), 1638-1642 (2008). TLC of Shujin Huoxue capsule extracts on silica gel with 1) petroleum ether (60-90 ºC) - ethyl formate - formic acid 15:5:1; 2) cyclohexane - ethyl acetate 9:1; 3) toluene - acetone - ethanol - ammonia 16:12:1:4. Detection 1) by exposure to iodine vapor; 2) under UV 365 nm; 3) under UV 254 nm. Identification by comparison with the standards of the component drugs. Quantification of paeoniflorin by HPLC.