J. Chinese Trad. Patent Med. (Zhongchengyao) 17 (7), 18-19 (1995). TLC of gardenoside, scutellarin, berberine on silica with 1) ethyl acetate - butanone - acetic acid - water 10:7:5:3, 2) petrol ether - ethyl acetate - formic acid 15:5:1, 3) benzene - ethyl acetate - methanol - isopropanol - NH3 12:6:3:3:1. Detection by a) spraying with 2 % FeCl3 in ethanol, b) by exposing to NH3 vapor, c) under UV 365 nm. Quantification of berberine and gardenoside by densitometry at 345 nm and 218 nm.

Pharm. Pharmacol. Lett. 5 (3), 96-97 (1995). TLC on silica with benzene - methanol - acetic acid 35:4:2. Detection by exposure to iodine vapor, and by densitometry at 460 nm. Identification by SIMCA method using the migration data and peak heights of the components. Use of the method for the quality control of the medicines.

GIT Fachz. Lab. 2, 96-98 (1998). HPTLC of hydrocortisone and cinchocaine (2-butoxy-4-quinolinecarboxylic acid(2-diethylamino)-ethylamide) after 15 min chamber saturation on silica gel with toluene - acetone - acetic acid - ethanol 40:30:5:2. Densitometry at 240 nm (absorbance). The intermediate precision (n = 3) was found to be at 0.53% (peak height) and 1.7% (peak area), respectively for hydrocortisone, and at 1.6% (peak height) and 3.3% (peak area), respectively for cinchocaine. Rapid and economic TLC procedure.

J. Chinese Trad. Patent Med. (Zhongchengyao) 25 (7), 538-540 (2004). TLC on silica gel with 1) petroleum ether - ethyl acetate - butanone - methanol - water 15:25:3:7:1; 2) petroleum ether - ethyl acetate - formic acid 15:5:1. Detection 1) under UV light, 2) by spraying with 5 % potassium iodobismuthate solution and heating at 105 ºC for 5 min. Identification by finger print technique. Quantification of betaine by densitometry at 514 nm.

CBS 86, 13-15 (2001). HPTLC on silica gel (prewashed with methanol) with THF - toluene - formic acid - water 16:8:2:1 for flavonoids and toluene - ethyl acetate 9:2 for diterpenes. Detection of flavonoids by dipping in natural products reagent followed by dipping in PEG 6000 solution. Detection of diterpenes by dipping in anisaldehyde reagent. Evaluation with video technology.

Indian Drugs 41 (1), 46-47, 2004. HPTLC on silica gel plates with n-propanol - formic acid - water 90:1:9. Rf of berberine was 0.39. Quantification by densitometry at 348 nm via linear regression in the range of 40-220 ng. Limit of quantification and limit of detection were found to be 30 ng and 10 ng, respectively. The herbal extract was found to contain 0.402 % w/w of berberine. The percentage of berberine in formulations I, II, III and IV were 0.0886 %, 0.0123 %, 0.0283 % and 0.0091 % w/w, respectively. The standardized method was found to be reproducible, accurate, precise and selective.

Chinese J. Pharm. Anal. (Yaowu Fenxi Zazhi) 24 (1), 30-34 (2004). TLC on silica gel with chloroform - acetone - diethylamine 5:4:1. Detection under UV 254 nm. Identification by standard comparison. Use of silver gel as the surface enhanced subtract placed on the separated spots for measurement of FT-SERS. Investigation of the minimum detection amount for the compound, and the difference between the SERS and solid spectra.

J. Chinese Trad. Patent Med. (Zhongchengyao) 25 (8), 680-682 (2004). TLC on silica gel with 1) ethyl acetate - formic acid - water - methanol 35:10:15:2; 2) benzene - ethyl acetate 19:1; 3) chloroform - ethyl acetate - methanol - water; 4) petroleum ether; 5) petroleum ether - ethyl acetate 17:3; 6) chloroform - methanol - water 13:7:2; 7) ethyl acetate - formic acid - water 10:2:3. Detection 1) under daylight, 2) by spraying with 5 % H2SO4 in ethanol and heating at 105 ºC, 3) by spraying with 5 % p-dimethylaminobenzaldehyde in 10 % H2SO4 in ethanol and heating at 105 ºC, 4) by spraying with 2 % 2,4-dinitrophenylhydrazine in ethanol, 5) by spraying with 1 % vanillin - H2SO4 solution, 6) by spraying with 5 % FeCI3 in ethanol, 7) by spraying with 2 % AICI3 in ethanol and under UV 365 nm, 8) under UV light. Identification by fingerprint techniques.