Chinese J. Mod. Drug Appl. 7 (17), 228-229 (2013). Zhuangyao Zhitong Heji compound oral liquid is a herbal TCM preparation to treat deficiency of the kidney and liver, chills and pain of the waist and knee, wind-cold-dampness arthralgia and low back injury. For quality control, TLC on silica gel with n-hexane – ethyl acetate 9:1, detection under UV 366 nm. Identification of Angelica sinensis, Ligusticum chuanxiong and Saposhnikovia divaricata roots by fingerprint comparison with reference drugs.

Chinese J. of Heilongjiang Med. 26 (3), 382-385 (2013). Gongxueting Keli granules are a herbal TCM preparation for treating women’s asthenia of both the spleen and kidney, and blood stasis due to menstruation and metrorrhagia. For quality control, TLC on silica gel 1) for Eclipta and the standard ecliptasaponin A, with dichloromethane – ethyl acetate – methanol – water 30:40:15:3, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones are visible in daylight; 2) for Leonurus Artemisia (Laur.) S. Y. Hu F and the standard stachydrine hydrochloride, with acetone – ethanol – hydrochloric acid 10:6:1, detection by heating at 105 °C for 15 min followed by spraying with 10 % sulfuric acid in ethanol and then heating, followed by spraying with 5 % potassium indobismuthate – 1 % ferric chloride in ethanol 10:1, viewing in daylight.

Beijing J. of Trad. Chinese Med. 31 (6), 458-460 (2012). Qieshisan medical powder is a herbal TCM preparation for the treatment of eczema and skin exudate erosion. For quality control 1) microscopy of Scutellaria baicalensis and Rheum palmatum L. (mixing the powder with chloral hydrate on a slide and heating until the slide looks transparent; 2) TLC of natural Indigo and the standards indirubin and indigo, on silica gel with toluene – chloroform – acetone 5:4:1, detection in daylight; 3) Quantification of baicalin by HPLC.

J. Planar Chromatogr. 27, 11-18 (2014). HPTLC of fructose (1), glucuronic acid (1), galacturonic acid (3), rhamnose (4), xylose (5), arabinose (6), and galactose (7) in the seeds of Ocimum basilicum on silica gel with isopropyl acetate - ethyl acetate - ethanol - water 50:40:10:1. Detection by dipping into aniline diphenylamine o-phosphoric acid reagent (20 % of o-phosphoric acid [85%] added to 1:1 mixture of 2 % solutions of diphenylamine and aniline in acetone), followed by heating at 110 ºC for 5 min. Quantitative determination by absorbance measurement at 630 nm except for (4) which was determined at 370 nm. The hRF values for (1) to (7) were 80, 58, 51, 40, 25, 18 and 9, respectively.

J. Planar Chromatogr. 27, 113-119 (2014). HPTLC of sulphadiazine sodium (1) and trimethoprim (2) in medicated fish feed, fish tissues, and in their veterinary pharmaceutical formulation on silica gel with chloroform - toluene - ethanol - glacial acetic acid 9:9:2:2. Quantitative determination by absorbance measurement at 270 nm. The hRF values for (1) and (2) were 48 and 16, respectively. Linearity was in the range of 100-2000 ng/zone for (1) and 100-1000 ng/zone for (2). The intermediate/interday/intra-day precisions were below 3 % (n=3). Mean recoveries for (1) and (2) were 99.6 and 97.9 %, respectively.

Chinese J. of Inform. on TCM 21 (1), 88-90 (2014). Ziyin Mingmu pian tablet is herbal TCM for promoting blood circulation which is used for the treatment of primary pigmentary degeneration of retina and other eye diseases. For quality control, TLC on silica gel 1) for Paeonia suffruticosa Andr., with cyclohexane – ethyl acetate – glacial acetic acid 40:10:1, detection by spraying with 2 % vanillin in sulfuric acid – ethanol 1:10 and heating at 105 °C until the zones are visible in daylight and under UV 366 nm; 2) for Cornus officinalis Sieb. et Zucc., with toluene – ethyl acetate – formic acid 40:8:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones are visible in daylight; 3) for Notopterygium incisum, with cyclohexane – benzene - ethyl acetate 2:1:1, detection by spraying with 1 % vanillin in sulfuric acid – ethanol 1:4 and under UV 366 nm; 4) Acorus tatarinowii, with petroleum ether (60-90 °C) – ethyl acetate 4:1, detection under UV 366 nm. Quantification of salvianolic acid B by HPLC.

Chinese J. of Northwest Pharm. 28 (3), 231-235 (2013). Berberis heteropoda Schrank, a Xinjiang special medicinal herb, is a perennial shrub, produced in northwest and southwest China, and is widely used as a herbal TCM drug. It is used for the treatment of dysentery, diarrhea, swollen eyes, eczema, etc. For quality control, identification of three alkaloids by TLC of the sample extracts and the standards berberine hydrochloride, palmatine chloride and jatrorrhizine hydrochloride on silica gel with toluene – ethyl acetate – methanol – isopropanol – ammonia 12:6:4:3:1 (determined by investigating three mobile phase systems), detection under UV 366 nm. In addition to optimization of the stationary phase, the developing system and the detection technique, the method was validated by investigating the effects of the sample application volume (2-3 μL), the developing temperature (room temperature) and the relative humidity (42-65 %).

J. of China Pharm. 22 (3), 13-15 (2013). Gushangling Jiaonang capsule is a herbal TCM for post traumatic functional recovery, prescribed to treat muscle atrophy, bone hyperplasia, neck and lumbar diseases, nerve injuries etc. For quality control, HPTLC on silica gel 1) for Paeonia lactiflora Pall., with chloroform – ethyl acetate – methanol – formic acid 200:25:50:1, detection by spraying with 3 % vanillin in sulfuric acid – water 1:200 and heating mildly until the zones are visible in daylight; 2) for Angelica sinensis, with n-hexane – ethyl acetate – 9:1, detection under UV 366 nm; 3) Astragalus membranaceus (Fisch.) Bunge., with chloroform – methanol – water 13:7:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones are visible in daylight.