J. Planar Chromatogr. 22, 109-113 (2009). HPTLC of purpurin on silica gel with toluene - ethyl acetate - formic acid 98:2:1 in a twin trough chamber saturated for 20 min at 25 +/- 2 °C. Quantitative determination by absorbance measurement at 255 nm. The limit of detection and quantification was 50 and 100 ng/band, respectively.

by HPTLC. Abstract No. 9191, IHCB (2009). HPTLC of (-)hydroxy citric acid (the main constituent of Garcinia gummigutta fruits) on silica gel with n-propanol - water - glacial acetic acid 50:50:1. Quantitative determination by absorbance measurement at 210 nm. The hRf value was 46. The method was linear in the range of 100-1000 ng/spot, recovery was 99.8-100.9 %.

Food Addit. Contam. 24, 351-359 (2007). Inter-laboratory validation of the analytical method (published in the SIMBAG-FEED report 4.6) based on TLC coupled to bio-autography for the detection of tylosin, spiramycin and virginiamycin in feeding-stuffs for poultry, pig, cattle and calf. The detection limit of spiramycin was 2 mg/kg and the method has a target concentration of 1 mg/kg for tylosin and virginiamycin. The method showed high specificity and offers the possibility for screening before LC/MS analysis.

Ind. J. Pharma. Sci. 8(4), 198-201(2009). HPTLC of nebivolol hydrochloride and valsartan on silica gel with ethyl acetate - methanol - acetic acid 12:2:1 in a twin trough chamber saturated for 15 min. Quantitative determination by absorbance measurement at 240 nm for valsartan and 280 nm for nebivolol hydrochloride. The method was found to be linear in the range of 600-1400 ng/band for valsartan 1200-2800 ng/band for nebivolol. The sample were subjected to different stress conditions (acid, alkali, oxidation, photolysis, thermal) and all degradation products were well separated from the main compounds.

60th Indian Pharmaceutical Congress PA-220 (2008). HPTLC of domperidone and esomeprazole on silica gel with chloroform - methanol 9:1 with chamber saturation for 30 min. The hRf value was 25 for domperidone and 46 for esomeprazole. Quantitative determination by absorbance measurement at 295 nm. The method was linear in the range of 60-300 ng/spot for domperidone and 80-400 ng/spot for esomeprazole.

Abstract No. F-269, 61st IPC (2009). HPTLC of hydrochlorothiazide and irbesartan on silica gel with acetonitrile - chloroform 5:6. The hRf value was 27 and 45 for irbesartan and hydrochlorothiazide, respectively. Quantitative determination by absorbance measurement at 270 nm. The sample was exposed to different stress conditions (acid, alkali, oxidative, photodegradation, thermal). Neither of the compounds showed degradation under thermal and photodegradation conditions, but both compounds showed significant degradation under acid, alkali and hydrolytic conditions. Degraded products were well resolved from the parent compounds.

J. Planar Chromatogr. 22, 439-443 (2009). HPTLC of jatrorrhizine in methanolic extracts of the stem of the ’heavenly elixir’ on silica gel with ethyl acetate - isopropanol - 10 % ammonia 3:5:3 in a twin trough chamber saturated for 5 min. Detection under UV 254 nm and after derivatization with Dragendorff’s reagent. Quantitative determination by absorbance measurement at 344 nm.

J. Planar Chromatogr. 22, 359-362 (2009). HPTLC of aloenin in aloe juice, tablets, and liquid extracts on silica gel with ethyl acetate - 95 % ethanol - water 20:3:1 at room temperature in a saturated chamber. Detection by immersion for 1 s in freshly prepared 5 % sodium hydroxide solution in 95 % ethanol, followed by heating at 100 °C for 5 min. Quantitative determination by absorbance measurement at 365 nm.