Indian Drugs 34 (8), 433-436 (1997). HPTLC on silica gel with dichloromethane - acetone - methanol - triethylamine 70:40:50:2. Densitometry at 257 nm.

J. Chinese Trad. Patent Med. (Zhongchengyao) 25 (7), 526-529 (2004). TLC on silica gel with benzene - ethyl acetate - methanol - isopropanol - conc. ammonia 20:10:5:5:1. Detection of berberine chloride under UV 365 nm. Identification by standard comparison. Quantification by densitometry at 342 nm. GC of volatile oil and HPLC of cinnamyl aldehyde. Discussion of the optimization of the extraction methods of combined ingredients of the medicine.

J. Chinese Trad. Patent Med. (Zhongchengyao) 25 (9), 715-718 (2004). TLC on silica gel with 1) ethyl acetate - butanone - formic acid - water 5:3:1:1, 2) benzene - ethyl acetate - methanol - isopropanol - conc. ammonia 12:6:3:3:1, 3) chloroform - acetone 19:1, 4) n-butanol - glacial acetic acid - water 7:1:2. Detection 1) by spraying with 5 % FeCI3 in ethanol, 2) spraying with 5 % vanillin - H2SO4 solution and heating at 100 ºC for 10 min, 3) under UV 365 nm. Identification by fingerprint techniques. Quantification of berberine hydrochloride by densitometry at 340 nm.

INDIAN DRUGS 41(1), 32-35, 2004. HPTLC on silica gel 60 F254 aluminium foil with toluene - methanol - chloroform 4:5:11 with chamber saturation for 30 min at room temperature. Rf of amlodipine besylate and losartan potassium was found to be 0.14 and 0.32, respectively. Quantification by densitometry at 245 nm via linear regression for amlodipine besylate between 0.05 to 0.10 µg and for losartan potassium between 0.5 to 1 µg. Validation regarding to accuracy and precision was performed. The limit of detection and limit of quantification for amlodipine besylate was found to be 0.01 and 0.03 ng/µL - for losartan potassium it was 0.004 and 0.013 ng/µL. The average recovery was determined to be 101.15% for amlodipine besylate and 100.74% for losartan potassium.

J. Chinese Trad. Patent Med. (Zhongchengyao) 11, 882-884 (2004). TLC on silica gel with 1) chloroform - methanol - conc. ammonia 80:4:1, 2) benzene - ethyl acetate 20:1, 3) chloroform - methanol - water 13:6:2, 4) ethyl acetate - formic acid - water 10:1:1. Detection 1) by spraying with 5 % solution of potassium iodobismuthate, 2) by spraying with 10 % H2SO4 in ethanol and heating at 105 ºC, 3) by spraying with 10 % H2SO4 in ethanol, heating at 105 ºC and under UV 365 nm, or under 254 nm. Identification by fingerprint techniques. Quantitative determination of matrine by densitometry at 520 nm. Validation of the quantitative method by investigation of its linearity range, reproducibility, repeatability, recovery.

CBS 93, 10-12 (2004). HPTLC of progesterone on silica gel prewashed by development in AMD2 first with chloroform - methanol 1:1 and then with the mobile phase, followed by drying at 80 °C for 15 min. Development in AMD2 with toluene - 2-propanol 10:1 without preconditioning over 60 mm. Quantitative determination by absorbance measurement at 252 nm followed by spectra recording from 200 to 360 nm. The linear working range is 25.7-154.5 ng/zone. Repeatability (standard deviation calculated from the amounts of seven simulated progesterone samples determined on the same plate at three concentration levels in the lower, middle and upper range) is 0.26-1.29 %. Recovery is 99.88-100.97 %. Reproducibility was performed with recycled HPTLC plates.

Indian Drugs 41 (3), 160-164 (2004). Combinations of ofloxacin and tinidazole (1:3) are available as tablet dosage forms. Stability indicating HPTLC of ofloxacin and tinidazole on silica gel with dioxan - ethyl acetate - toluene - acetic acid - water 5.5:5:3.2:2:2 . Quantification by absorbance measurement at 307 nm. The Rf value of ofloxacin and tinidazole was found to be 0.12 and 0.85 respectively. Recovery was 99.85 % and 99.45 % for ofloxacin and tinidazole respectively. The proposed method was quantitatively evaluated in terms of calibration of concentration range, stability, and accuracy. It can be adopted for routine analysis of the formulation.

Indian J. Pharm. Sci. 66 (3), 278-282 (2004). HPTLC on silica gel with chloroform - acetonitrile - toluene - acetate buffer (pH 6.0) 50:40:10:3. Quantitative determination by absorbance measurement at 266 nm. The method was validated in terms of linearity, accuracy, precision, and specificity. The limit of detection and the limit of quantification were found to be 50 ng/spot and 100 ng/spot respectively. A simple, precise, accurate and rapid HPTLC method has been developed and validated for the simultaneous determination of cefuroxime axetil and probenecid in combined dosage form.