J. Planar Chromatogr. 25, 251-258 (2012). TLC of trans-resveratrol (1), pterostilbene (2), and p-coumaric acid (3) in samples of recombinant bacterial cultures, food supplements, and wine on silica gel with n-hexane - ethyl acetate - formic acid 20:19:1. Quantitative determination by absorbance measurement at 286 nm (3) and 303 nm for (1) and (2).

J. of Yangtze Univ. (Nat. Sci., Med.) 9 (1), 64-65 (2012) . Angelica sinensis is a herbal TCM drug widely used in the preparations for gynaecological diseases, effective in promoting blood circulation, ease of pain and against inflammations. TLC of the extracts of the preparation on silica gel with n-hexane – ethyl acetate 9:1. Detection under UV 366 nm.

J. Liq. Chromatogr. Relat. Technol. 35, 1459-1480 (2012). HPTLC of itraconazole on silica gel with toluene - ethyl acetate 1:5 + 1 drop ammonia. Quantitative determination by absorbance measurement at 266 nm. The hRf value of itraconazole was 77 and selectivity regarding matrix was given. Linearity was in the range of 50-2000 ng/zone. The intra-day and inter-day accuracy were in the range of 81.9-97.8 and 89.2-97.1 %, respectively. The limits of detection and quantification were 14 and 43 ng/zone, respectively. Recovery (by standard addition) was between 99.6 and 100.2 %.

Chinese J. of Guide for Trad. Chinese Med. & Pharm. 18 (7), 78-79 (2012). Zhilou fumigating lotion is a herbal TCM preparation for treatment of external hemorrhoids and hemorrhoid postoperation hemorrhage. TLC of the extracts of the preparation 1) for Rheum officinale, on silica gel with the upper phase of petroleum ether (30-60 ºC) – ethyl formate – formic acid 15:5:1, detection at UV 366 nm; 2) for Rhizoma Polygoni Cuspidati, on silica gel with chloroform – acetone – formic acid 16:3:1, detection at UV 366 nm; 3) for Chinese gall, on silica gel with chloroform – formyl acetate – formic acid 5:5:1, detection at UV 254 nm; 4) for Herba Houttuyniae, on silica gel with toluene – ethyl acetate – formic acid – water 20:10:1:1, detection at UV 366 nm; 5) for Salvia plebeia R.Br., on polyamide phase with methanol – acetic acid – water 13:3:4, detection at UV 366 nm.

J. of Modern Trad. Chinese Med. 14 (7), 11-13 (2012). Olibanum is the resin from the bark of Boswellia carterii Birdw. and Boswellia bhaw. dajiana Birdw. It containis triperpenoids such as 11-keto-beta-boswellic acid (KBA), and acetyl-11-keto-beta-boswellic acid (AKBA) which show antiinflammatory and antibiotic activity. In order to discern the false from the genuine drug in market, a method for the quality control of Olibanum produced in Indonesia, Ethiopia, Somalia and India has been presented. TLC on silica gel with cyclohexane – ethyl acetate – glacial acetic acid 50:10:1, detection at UV 254 nm.

J. Liq. Chromatogr. Relat. Technol. 35, 1565-1584 (2012). HPTLC of negundoside (1), ursolic acid (2), eugenol (3), lupeol (4), and beta-sitosterol (5) in the leaves of Vitex Negundo on silica gel with toluene - methanol 9:1. Detection by dipping in anisaldehyde sulfuric acid reagent for approximately 1 min, followed by heating at 100 °C for 5 min. Quantitative determination by absorbance measurement at 525 nm. The hRf value of compounds (1) to (5) were 47, 61, 56, 54 and 38, and selectivity regarding matrix was given. Linearity was in the range of 200-800 ng/zone for (1), 72-576 ng/zone for (2), 200-1000 ng/zone for (3), 150-900 ng/zone for (4) and 80-480 ng/zone for (5). Limits of detection and quantification were 80 and 200 ng/zone for (1), 18 and 72 ng/zone for (2), 60 and 200 ng/zone for (3), 50 and 100 ng/zone for (4) and 20 and 60 ng/zone for (5), respectively. The method provides acceptable intra-day and inter-day precision for (1) to (5). The average recoveries for compounds (1) to (5) were found to be 99.9, 100.1, 99.7, 100.0, and 99.9 %, respectively.

J. Chromatogr. A 1266, 168-174 (2012). RP-TLC plates modified with C18, C8 or C2 are available on the market, however, RP-plates with tunable polarity have not been reported. Because of the limited variety of RP-plates mobile phase optimization is needed which is often time consuming. The presented polarity-adjustable RP-UTLC plates simplify the mobile phase screening process and expand the selection of reversed phase plates. The plates were prepared using the glancing angle deposition (GLAD) technique to deposit SiO2 nanopillars on glass plates and functionalized with octadecyltrichlorosilane for hydrophobicity. By O2 plasma treatment the silane carbon chain was shortened and COOH groups were introduced to the surface, producing plates with finely tunable polarities. Confirmation of the tuning of surface polarities by measurement of retention behavior changes in the separation of a model dye mixture of Sudan blue and Sudan IV, the elution order of which reversed as a result of the change in surface polarity. Testing on commercial RP-plates showed no change in the separation behavior which proves that plasma treatment of GLAD structures with highly accessible surfaces improves control over interfacial properties, producing better reverse phase separations. UTLC of Sudan blue and Sudan IV in toluene on the new RP-UTLC plates in horizontal developing chamber with acetonitrile, detection in visual light by recording of the development process by video camera).

J. Planar Chromatogr. 26, 86-92 (2013). HPTLC of carteolol on silica gel with chloroform - methanol 5:1. Quantitative determination by absorbance measurement at 254 nm. The hRf of carteolol was 31. Linearity was in the range of 200-1200 ng/zone. LOD and LOQ were 34 ng/zone and 104 ng/zone, respectively. Intermediate precision was below 2 %. The method could effectively separate the drug from its degradation products.