J. Chinese Trad. Patent Med. (Zhongchengyao) 25 (8), 680-682 (2004). TLC on silica gel with 1) ethyl acetate - formic acid - water - methanol 35:10:15:2; 2) benzene - ethyl acetate 19:1; 3) chloroform - ethyl acetate - methanol - water; 4) petroleum ether; 5) petroleum ether - ethyl acetate 17:3; 6) chloroform - methanol - water 13:7:2; 7) ethyl acetate - formic acid - water 10:2:3. Detection 1) under daylight, 2) by spraying with 5 % H2SO4 in ethanol and heating at 105 ºC, 3) by spraying with 5 % p-dimethylaminobenzaldehyde in 10 % H2SO4 in ethanol and heating at 105 ºC, 4) by spraying with 2 % 2,4-dinitrophenylhydrazine in ethanol, 5) by spraying with 1 % vanillin - H2SO4 solution, 6) by spraying with 5 % FeCI3 in ethanol, 7) by spraying with 2 % AICI3 in ethanol and under UV 365 nm, 8) under UV light. Identification by fingerprint techniques.

J. AOAC Int. 86, 935-940 (2003). TLC of indapamide (4-chloro-N-(2-methylindolin-1-yl)-3-sulfamoylbenzamide), its degradation product, and related substance 2-methylnitrosoindoline on silica gel using toluene - ethyl acetate - glacial acetic acid 69:30:1 after pre-saturation for 1 h. Detection under UV light at 254 nm. Quantitative determination at 424 nm. Limit of detection 0.11 µg/spot. The method was validated according to the guidelines of the USP.

J. Planar Chromatogr. 17, 147-148 (2004). TLC of capsaicin on silica gel with concentration zone with toluene - acetone - chloroform 8:7:5 and 9:6:5. Detection by spraying with a 0.25 % ethanolic solution of 2,6-dichloroquinone-4-chlorimide or by exposure to iodine vapor.

Chinese J. Trad. Patent Med. (Zhongchengyao) 26 (7), 597-600 (2004). TLC of Qieban Zhiyang ointment extracts on silica gel with 1) benzene - ethyl acetate 10:1; 2) petroleum ether (60-90 ºC) - ethyl formate - formic acid 15:5:1; 3) chloroform - methanol - ammonia 40:3:1; 4) benzene - chloroform - acetone 5:4:1; 5) chloroform - methanol 19:1. Detection 1) under UV 365 nm; 2) by exposing to ammonia vapor; 3) by spraying with potassium iodobismuthate solution; 4) under daylight. Identification by fingerprint technique. Quantification of icariine by HPLC.

J. Chinese Pharm. Anal. 25 (1), 86-90 (2005). TLC of fructose, fructose-6-phosphate and related impurities on silica gel - carboxy methyl cellulose(CMC) -Na phase with n-butanol - acetone - glacial acetic acid - ammonia - water 35:15:20:3:27. Detection by spraying with 1 % sodium periodate solution followed by spraying with a solution of benzidine - ethanol - acetone - hydrochloric acid - water 0.8 g:80 mL:30 mL:1.5 mL:70 mL. Identification by fingerprint technique. Quantification by comparison with standards. The detection limits were investigated. In addition, the content of magnesium fructose diphosphate was determined by diphenylamine colorimetric method, and the related impurities are determined with the phospho-molybdic acid colorimetric method.

Chinese J. Trad. Pat. Med. (Zhongchengyao) 26 (9), app.17-19 (2004). HPTLC on silica gel with 1) toluene - acetone - ethanol - ammonia 20:25:3:2; 2) n-hexane - ethyl acetate - glacial acetic acid 15:5:1; 3) n-hexane - ethyl acetate - ammonia 20:20:1. Detection 1) by spraying with 5 % potassium iodobismuthate solution; 2) by spraying with 1 % potassium permanganate in diluted sulfuric acid followed by heating at 120 ºC, and under UV 360 nm. Identification by fingerprint technique. Determination of the content of aconitine by comparison with standard.

J. Planar Chromatogr. 17, 238-240 (2004). HPTLC of aceclofenac and mosapride citrate (as internal standard) on silica gel in a twin-trough chamber equilibrated with the mobile phase with toluene - methanol - ethyl acetate - glacial acetic acid 550:250:200:1. Quantitative determination by densitometry at 284 nm.

J. Planar Chromatogr. 18, 221-223 (2005). HPTLC of horminone on silica gel in a twin-trough chamber with hexane - dioxane 9:1. Absorbance measurement at 271 nm. For fluorescence analysis the plates were dipped in 1 % diphenylboryloxyethylamine in ethyl acetete for 2 s, followed by drying and dipping in a solution of 5 % PEG 8000 in dichloromethane for 2 s. After 15 min fluorescent zones of horminone were scanned at 366/>400 nm. Use of the fluorescence reagent successfully reduced the limits of detection and quantification to 0.75 ng/spot and 1.51 ng/spot, respectively. Linearity range was from 60-300 ng/spot.