Abstract No. F-315, 61st IPC (2009). HPTLC for pravastatin on silica gel with ethyl acetate - toluene - acetonitrile - formic acid 60:35:5:2. Quantitative determination by absorbance measurement at 237 nm. The method was linear in the range of 318-3816 ng/band. Recovery was 99.9-101.2 %. Stability tests showed that degradation products resulting under acid stress conditions were well resolved from the main component.

60th Indian Pharmaceutical Congress PG-246 (2008). HPTLC of biomarkers such as curcumin, charantin, and swertiamarin in some polyherbal formulations on silica gel with benzene - methanol 4:1 (for charantin), chloroform - methanol - formic acid 74:4:1 (for curcumin), and ethyl acetate - methanol - water 77:15:5 (for swertiamarin). Quantitative determination by absorbance measurement at 536 for charantin (hRf value 33), 425 nm for curcumin (hRf value 89), and 238 nm for swertiamarin (hRf value 54).

Chinese J. Pharm. Anal. 28 (1), 20-23 (2008). TLC of TCM drug extracts on silica gel with toluene – ethyl acetate – methanol – isopropanol – ammonia 12:6:3:3:1. Detection under UV light. The method is suitable for quality control of Baozhi pills.

60th Indian Pharmaceutical Congress PA-223 (2008). HPTLC of curcumin, demethoxycurcumin and bis-demethoxycurcumin on silica gel with chloroform - methanol 19:1. The hRf values were 25, 38, and 61 for bis-demethoxycurcumin, demethoxycurcumin, and curcumin respectively. Quantitative determination by absorbance measurement at 420 nm. The method was linear in the range of 50-400 ng/spot (curcumin), 10-150 ng/spot (demethoxycurcumin), and 5-40 ng/spot (bis-demethoxycurcumin). Recovery was in the range of 99.2-100.5 % for all three compounds.

60th Indian Pharmaceutical Congress PA-229 (2008). HPTLC of voriconazole on silica gel with toluene - methanol - glacial acetic acid 78:20:1. Quantitative determination by absorbance measurement at 254 nm. The method was linear in the range of 50-400 ng/spot, recovery was 99.9-100.7 %. The method was suitable for routine quality control of the dosage form.

IPA Convention, 2010, RA-PO 26. HPTLC of risedronate sodium on silica gel with water - methanol - 25 % ammonia 20:3:3. Densitometric evaluation at 262 nm. The method was linear in the range of 3-6 ng/band, recovery was 98.6 %. Results were comparable with HPLC results. The advantage of the HPTLC method is the simultaneous analysis of several samples.

J. Liq. Chromatogr. Relat. Technol. 31, 2429-2441 (2008). TLC of citalopram, sertraline, fluoxetine, and fluvoxamine on silica gel with acetone - benzene - 25 % ammonia 10:9:1 in a twin-trough chamber saturated for 15 min. Detection under UV 254 nm. The hRf value was 28 for fluoxetine, 44 for citalopram, 56 for fluvoxamine, and 68 for sertraline (with a standard deviation less than 0.02 % in all cases). Quantitative determination by absorbance measurement at 240 nm. The calibration curve was linear in the range of 500-5000 ng/spot for all analyzed compounds; correlation coefficients were found to be more than 0.998 for all drugs (except the 0.991 for fluvoxamine). LOD was 40 ng/spot for citalopram and 50 ng/spot for fluoxetine, fluvoxamine, and sertraline, respectively. LOQ was found to be 130 and 160 ng/spot for citalopram and fluoxetine, respectively. Intra-assay precision (% RSD) was within the range of 0.52-0.87 % and 1.34-1.84 % for citalopram and fluoxetine, respectively, inter-day precision for the analyses conducted on three consecutive days was below 1.8 % and 2.5 % for citalopram and fluoxetine, respectively. The recoveries (RSD) of citalopram ad fluoxetine were found to be in the range of 99.3-100.3% (0.8 %) and 99.4-100.4 % (1.9%), respectively.

Abstract No. C-97, 61st IPC (2009). Chromatographic methods are reported for identification (TLC) and quantification (HPTLC) of withaferin-A in methanolic and chloroform extract of dried fruits of Withania coagulans. Chromatographic separation on silica gel with toluene - ethyl acetate - formic acid 5:5:1. The identification of withaferin-A in both chloroform and methanolic extracts was performed by comparison of hRf values and UV absorbance maxima (209 nm). Quantification was performed by absorbance measurement at 540 nm after spraying the developed plate with Liebermann-Burchard reagent. Methanolic extracts and chloroform extracts contained 3.67 mg/g and 2.10 mg/g of withaferin-A, respectively. No withaferin-A was found in hydroalcoholic extracts.