J. Planar Chromatogr. 24, 344-347 (2011). HPTLC of ivermectin (IVM) and albendazole (ALB) on silica gel with toluene - ethyl acetate - glacial acetic acid 12:8:1 in a twin-trough chamber saturated for 30 min. Quantitative determination by densitometry in absorbance mode at 247 nm. Linearity was between 0.12 and 0.54 µg/band for IVM and 8 and 36 µg/band for ALB. The recovery was between 98-101 % for IVM and ALB. The hRf value was 39 for IVM and 62 for ALB. LOD and LOQ were 0.02 and 0.09 µg/band for IVM and 0.08 and 0.1 µg/band for ALB. The intra-day and inter-day precision (n = 6) was 0.6 % and 1.1 % for IVM and 0.6 % and 1.2 % for ALB, respectively. Recovery (by standard addition) ranged from 98-101 % for both compounds.

J. Planar Chromatogr. 25, 320-325 (2012). HPTLC of ursolic acid in the leaves of Diospyros melanoxylon on silica gel with chloroform - methanol 19:1. Detection by dipping in 5 % methanolic sulphuric acid reagent and heating at 105 °C for 3 min. Quantitative determination by absorbance measurement at 540 nm. Linearity was in the range of 50-450 ng/zone for ursolic acid. Limits of detection and quantification were found to be 20 and 40 ng/zone. Recovery (by standard addition) was 97.5 %.

J. Liq. Chromatogr. Relat. Technol. 35, 1731-1749 (2012). HPTLC of atazanavir sulfate (1) and ritonavir (2) in fixed dosage on silica gel with toluene – methanol – glacial acetic acid – ethyl acetate 14:1:3:4. Quantitative determination by absorbance measurement at 254 nm. The hRf values of (1) and (2) were 50 and 63, respectively. Linearity was 30-300 ng/zone for (1) and 10-100 ng/zone for (2). The intermediate/inter-day/intra-day precision was 0.3 % for (1) and 0.7 % for (2) (n=6). The limit of detection and quantification was 16 and 49 ng/zone for (1) and 18 and 55 ng/zone for (2), respectively. Recovery (by standard addition) was 99.9 % for (1) and (2). The results were in accordance with those by a validated HPLC method.

J. Planar Chromatogr. 25, 426-432 (2012). HPTLC of shanzhiside methyl ester (1) and barlerin (2) on silica gel with chloroform - methanol 4:1. Quantitative determination by absorbance measurement at 240 nm. The hRf of compounds (1) and (2) were 30 and 48, respectively. Linearity was in the range of 200-1000 ng/zone. Limits of detection and quantification were found to be 13 and 22 ng/zone for (1) and 18 and 31 ng/zone for (2), respectively. Recovery was found to be 99.2-99.5 % for (1) and 98.9-99.2 % for (2), respectively. The method showed comparable results to a validated HPLC method.

J. of Jinan Univ. (Natural Sci.) 33 (3), 289-293 (2012). Compound Shuyu granule is a herbal TCM preparation prescribed clinically to treat insomnia, neurosis and melancholia. TLC of the extracts of the medicine on silica gel 1) for Caulis Polygoni Multiflori and the standard emodin, with the upper phase of petroleum ether (60-90 ºC) – formyl acetate – formic acid 15:5:1, detection under UV 366 nm and by exposiure to iodine vapors and viewing under UV 366 nm; 2) for Salvia miltiorrhiza and the standard salvianolic acid B, with cyclohexane – dichloromethane – acetone – formic acid – glacial acetic acid 2:4:2:2:1, detection by spraying with 5 % ferric chloride in ethanol and heating at 105 ° until the zones are visible; 3) for white Paeony root, with chloroform – ethyl acetate – methanol – formic acid 200:25:50:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:200 and heating mildly until the zones are seen.

J. of China Pharm. 25 (8), 772-775 (2011). Safflower, the dried flower of Carthamus tinctorius L. is a herbal TCM drug for invigorating the circulation of blood, stimulating the menstrual flow, dissipating blood stasis, relieving pain, and is prescribed clinically to cure amenorrhea, falling injuries and skin and external diseases. Due to the lack of the source some counterfeits have been found on the market in recent years. The methods were studied for differentiating the dyes used by the market for adulteration of safflower. For dyes, TLC of the extracts of the crude drugs on silica gel firstly with chloroform – methanol – glacial acetic acid 7:1:2, detection under daylight for identification of orange, then with ethyl acetate – n-butanol – ethanol – ammonia – water 1:3:3:1:1, detection under daylight for identification of acid red 73, lemon yellow and carminum respectively. Results obtained by HPLC were compatible with those obtained by TLC.

J. of China Pharm. 21 (3), 16-18 (2012). Dingxuan granules are a herbal TCM preparation with nourishing effect on liver and kidney and curative effect on Meniere's syndrome. For quality control, TLC of the extracts of the medicine 1) for Radix Polygoni multiflori preparata, on silica gel first over 3.5 cm with chloroform – methanol 7:3, then over 7 cm with chloroform – methanol 20:1, detection at UV 366 nm; 2) for Radix Paeoniae alba, on silica gel with chloroform – methanol 4:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 4:1 and heating at 105 °C, viewing under daylight; 3) for the fruit of Chinese wolfberry, on silica gel developed with chloroform – ethyl acetate – formic acid 2:3:1, detection at UV 366 nm; 4) for Angelica sinensis, on silica gel with n-hexane – ethyl acetate 17:3, detection at UV 366 nm.

Chromatogr. 26 (1), 61–68 (2012) Picroside-I and picroside-II are known bioactive metabolites in Picrorhiza species. Presentation of a simple, precise method for the simultaneous determination of picrosides (picroside-I and picroside-II) in two different Picrorhiza species, P. kurroa and P. scrophulariiflora. TLC of the extracts of the medicinal herbal drugs on silica gel with chloroform – methanol 22:3. Quantification of picrosides (picroside-I and picroside-II) by absorbance measurement at UV 254 nm. Comparative study revealed that the content of picroside-I and picroside-II is higher in P. scrophulariiflora than P. kurroa the content of picroside-I was found to be 1.3 and 1.6 % inP. kurroa and P. scrophulariiflora, and of picroside-II 0.5 and 0.6 %, respectively. Study of the antioxidant potential of the two Picrorhiza species using DPPH* radical reagent. The scavenging activities of P. kurroa and P. scrophulariiflora were 37.7 % and 34.3 %, respectively, at a concentration of 0.1 mg/mL.